(Q35925628)

11 August 2017

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title

The selection of high-producing cell lines using flow cytometry and cell sorting (English)

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11 August 2017

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Silvia Carroll

1

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11 August 2017

Mohamed Al-Rubeai

series ordinal

2

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11 August 2017

language of work or name

English

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publication date

1 November 2004

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Expert Opinion on Biological Therapy

11 August 2017

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11 August 2017

4

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11 August 2017

11

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11 August 2017

1821-1829

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describes a project that uses

11 August 2017

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Continuous cultures of fused cells secreting antibody of predefined specificity

1 reference

https://api.crossref.org/works/10.1517%2F14712598.4.11.1821

Characterization of the stability of recombinant protein production in the GS-NS0 expression system

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F14712598.4.11.1821

Amplification and expression of sequences cotransfected with a modular dihydrofolate reductase complementary DNA gene

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F14712598.4.11.1821

Effect of cloned gene dosage on cell growth and hepatitis B surface antigen synthesis and secretion in recombinant CHO cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F14712598.4.11.1821

Efficient selection of high-producing subclones during gene amplification of recombinant Chinese hamster ovary cells by flow cytometry and cell sorting

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F14712598.4.11.1821

Helpful hints in flow cytometry and sorting

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F14712598.4.11.1821

Single cell sorting and cloning.

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F14712598.4.11.1821

Antigen-specific identification and cloning of hybridomas with a fluorescence-activated cell sorter

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F14712598.4.11.1821

Selection of hybridomas and hybridoma variants using the fluorescence activated cell sorter

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F14712598.4.11.1821

Hybrid hybridomas producing bispecific antibodies to CEA and peroxidase isolated by a combination of HAT medium selection and fluorescence activated cell sorting.

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F14712598.4.11.1821

Rapid spontaneous dihydrofolate reductase gene amplification shown by fluorescence-activated cell sorting

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F14712598.4.11.1821

Relationship between loss of heavy chains and the appearance of nonproducing hybridomas

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F14712598.4.11.1821

Comparison of the production of a human monoclonal antibody against HIV-1 by heterohybridoma cells and recombinant CHO cells: A flow cytometric study

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F14712598.4.11.1821

Changes during subclone development and ageing of human antibody-producing recombinant CHO cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F14712598.4.11.1821

Flow cytometric study of hybridoma cell culture: correlation between cell surface fluorescence and IgG production rate

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F14712598.4.11.1821

The gel microdrop secretion assay: Identification of a low productivity subpopulation arising during the production of human antibody in CHO cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F14712598.4.11.1821

Isolation of rare isotype switch variants in hybridoma cell lines using an agarose gel microdrop-based protein secretion assay

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F14712598.4.11.1821

Analysis and sorting of live cells according to secreted molecules, relocated to a cell-surface affinity matrix

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F14712598.4.11.1821

Quantitating secretion rates of individual cells: design of secretion assays.

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F14712598.4.11.1821

Improved cell line development by a high throughput affinity capture surface display technique to select for high secretors

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F14712598.4.11.1821

Production of monoclonal antibodies using a secretion capture report web.

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F14712598.4.11.1821

Production of biologically active recombinant avidin in baculovirus-infected insect cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F14712598.4.11.1821

Introduction to avidin-biotin technology

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F14712598.4.11.1821

The X-ray three-dimensional structure of avidin

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F14712598.4.11.1821

Determination of efficiency of attachment of biotinylated antibodies to avidin-linked, aqueous-based microcapsules by flow cytometry

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F14712598.4.11.1821

VIGR--a novel inducible adhesion family G-protein coupled receptor in endothelial cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F14712598.4.11.1821

High-sensitivity immunofluorescence for detection of the pro- and anti-inflammatory cytokines gamma interferon and interleukin-10 on the surface of cytokine-secreting cells.

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F14712598.4.11.1821

Streptavidin contains an RYD sequence which mimics the RGD receptor domain of fibronectin

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F14712598.4.11.1821

Green fluorescent protein as a marker for gene expression

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F14712598.4.11.1821

Optimization of sorting conditions for the selection of stable, high-producing mammalian cell lines

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F14712598.4.11.1821

VEGF-Trap: a VEGF blocker with potent antitumor effects

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F14712598.4.11.1821

Cytokine traps: multi-component, high-affinity blockers of cytokine action

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F14712598.4.11.1821

10.1517/14712598.4.11.1821

21 January 2018

Identifiers

DOI

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11 August 2017

1 reference