(Q36076149)

12 August 2017

1 reference

title

Seamless cloning and gene fusion. (English)

1 reference

12 August 2017

Quinn Lu

1

1 reference

PubMed ID

15780712

12 August 2017

language of work or name

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1 April 2005

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12 August 2017

Trends in Biotechnology

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12 August 2017

23

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12 August 2017

4

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12 August 2017

199-207

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The N-end rule: functions, mysteries, uses

12 August 2017

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12 December 2020

NOMAD: a versatile strategy for in vitro DNA manipulation applied to promoter analysis and vector design

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12 December 2020

Genetic engineering using homologous recombination

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12 December 2020

Rapid gene cloning using terminator primers and modular vectors

1 reference

12 December 2020

Use of engineered ribozymes to catalyze chimeric gene assembly

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12 December 2020

High-throughput scanning mutagenesis by recombination polymerase chain reaction

1 reference

12 December 2020

Creating randomized amino acid libraries with the QuikChange Multi Site-Directed Mutagenesis Kit

1 reference

12 December 2020

Biotin-ubiquitin tagging of mammalian proteins in Escherichia coli

1 reference

12 December 2020

PCR fidelity of pfu DNA polymerase and other thermostable DNA polymerases

1 reference

12 December 2020

Increasing gene expression in yeast by fusion to ubiquitin.

1 reference

12 December 2020

Techniques: Recombinogenic engineering--new options for cloning and manipulating DNA.

1 reference

12 December 2020

A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions

1 reference

12 December 2020

Enzymatic inverse PCR: a restriction site independent, single-fragment method for high-efficiency, site-directed mutagenesis

1 reference

12 December 2020

N-terminal-methionylated interleukin-1 beta has reduced receptor-binding affinity.

1 reference

12 December 2020

Uracil DNA glycosylase-mediated cloning of polymerase chain reaction-amplified DNA: application to genomic and cDNA cloning

1 reference

12 December 2020

Three direct repeats and a TATA-like sequence are required for regulated expression of the human low density lipoprotein receptor gene

1 reference

12 December 2020

A critical review of the methods for cleavage of fusion proteins with thrombin and factor Xa.

1 reference

12 December 2020

In vivo half-life of a protein is a function of its amino-terminal residue

1 reference

12 December 2020

Minimum length of sequence homology required for in vivo cloning by homologous recombination in yeast

1 reference

12 December 2020

Production of first generation adenovirus vectors: a review

1 reference

12 December 2020

An Escherichia coli expression vector that allows recovery of proteins with native N-termini from purified calmodulin-binding peptide fusions

1 reference

12 December 2020

Integration of PCR fragments at any specific site within cloning vectors without the use of restriction enzymes and DNA ligase

1 reference

12 December 2020

Method of artificial DNA spling by directed ligation(SDL)

1 reference

12 December 2020

Systematic assembly of a full-length infectious cDNA of mouse hepatitis virus strain A59.

1 reference

12 December 2020

Semisynthesis of proteins by expressed protein ligation

1 reference

12 December 2020

Creating seamless junctions independent of restriction sites in PCR cloning

1 reference

12 December 2020

Modification of human beta-globin locus PAC clones by homologous recombination in Escherichia coli

1 reference

12 December 2020

Oligonucleotide-mediated, PCR-independent cloning by homologous recombination

1 reference

12 December 2020

Ligation-independent cloning of PCR products (LIC-PCR)

1 reference

12 December 2020

Influence of the amino acid residue downstream of (Asp)4Lys on enterokinase cleavage of a fusion protein

1 reference

12 December 2020

Ubiquitin fusion technique and its descendants

1 reference

12 December 2020

Using Schizosaccharomyces pombe as a host for expression and purification of eukaryotic proteins.

1 reference

12 December 2020

Site-directed mutagenesis of double-stranded DNA by the polymerase chain reaction

1 reference

12 December 2020

Universal restriction site-free cloning method using chimeric primers.

1 reference

12 December 2020

Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension

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12 December 2020

Design of highly specific cytotoxins by using trans-splicing ribozymes.

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12 December 2020

Thermodynamically balanced inside-out (TBIO) PCR-based gene synthesis: a novel method of primer design for high-fidelity assembly of longer gene sequences

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12 December 2020

Applications of DNA shuffling to pharmaceuticals and vaccines

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12 December 2020

The P1' specificity of tobacco etch virus protease

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12 December 2020

Production of authentic human proapolipoprotein A-I in Escherichia coli: strategies for the removal of the amino-terminal methionine

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12 December 2020

Transcriptional Control Signals of a Eukaryotic Protein-Coding Gene

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12 December 2020

A bicistronic expression system for bacterial production of authentic human interleukin-18.

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12 December 2020

Targeting, disruption, replacement, and allele rescue: integrative DNA transformation in yeast

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12 December 2020

Improved protocol for high-throughput cysteine scanning mutagenesis

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12 December 2020

Effect of site-specific modification on restriction endonucleases and DNA modification methyltransferases

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12 December 2020

Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli: application to open reading frame characterization

1 reference

12 December 2020

Rational design and engineering of therapeutic proteins

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12 December 2020

High-resolution epitope mapping of hGH-receptor interactions by alanine-scanning mutagenesis

1 reference

12 December 2020

Extension of recombinant human RANTES by the retention of the initiating methionine produces a potent antagonist.

1 reference

12 December 2020

A new protease required for cell-cycle progression in yeast

1 reference

12 December 2020

A new logic for DNA engineering using recombination in Escherichia coli

1 reference

12 December 2020

S. pombe expression vector with 6x(His) tag for protein purification and potential for ligation-independent cloning

1 reference

12 December 2020

Construction of long DNA molecules using long PCR-based fusion of several fragments simultaneously

1 reference

12 December 2020

Homologous recombination based modification in Escherichia coli and germline transmission in transgenic mice of a bacterial artificial chromosome.

1 reference

12 December 2020

PCR-based gene synthesis and protein NMR spectroscopy

1 reference

12 December 2020

Two-stage PCR protocol allowing introduction of multiple mutations, deletions and insertions using QuikChange Site-Directed Mutagenesis

1 reference

12 December 2020

Rapid cloning by homologous recombination in vivo

1 reference

12 December 2020

Construction of linker-scanning mutations using the polymerase chain reaction

1 reference

12 December 2020

DNA cloning using in vitro site-specific recombination

1 reference

12 December 2020

Class-IIS restriction enzymes--a review

1 reference

12 December 2020

Surface-accessible residues in the monomeric and assembled forms of a bacterial surface layer protein.

1 reference

12 December 2020

The degradation signal in a short-lived protein

1 reference

12 December 2020

10.1016/J.TIBTECH.2005.02.008

Identifiers

DOI

1 reference

12 August 2017

1 reference

PubMed ID

15780712