(Q36685587)

15 August 2017

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title

Large-scale transfection of mammalian cells for the fast production of recombinant protein (English)

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15 August 2017

Phuong Lan Pham

1

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15 August 2017

Amine Kamen

series ordinal

2

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15 August 2017

Yves Durocher

series ordinal

3

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15 August 2017

publication date

1 October 2006

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15 August 2017

published in

Molecular Biotechnology

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15 August 2017

volume

34

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15 August 2017

issue

2

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15 August 2017

page(s)

225-237

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https://scigraph.springernature.com/pub.10.1385/mb:34:2:225

15 August 2017

exact match

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cites work

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12 December 2020

Serum-free large-scale transient transfection of CHO cells

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Transfected insect cells in suspension culture rapidly yield moderate quantities of recombinant proteins in protein-free culture medium

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Protein expression in mammalian and insect cell systems

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Energy dependence of DNA-mediated gene transfer and expression

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Transient gene expression in HEK293 cells: peptone addition posttransfection improves recombinant protein synthesis

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Large scale transient expression with COS cells

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Adenovirus VA RNAI mediates a translational stimulation which is not restricted to the viral mRNAs

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100-liter transient transfection

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Large-scale transient expression in mammalian cells for recombinant protein production

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SEAP expression in transiently transfected mammalian cells grown in serum-free suspension culture

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On the mechanism of DNA transfection: efficient gene transfer without viruses

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Transient gene expression in mammalian cells grown in serum-free suspension culture

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12 December 2020

Simian virus 40 replication in adenovirus-transformed human cells antagonizes gene expression

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12 December 2020

Transfection of adherent and suspended cells by calcium phosphate

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12 December 2020

High efficiency transformation by direct microinjection of DNA into cultured mammalian cells

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12 December 2020

Calcium phosphate transfection optimization for serum-free suspension culture

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12 December 2020

An EBNA-1-dependent enhancer acts from a distance of 10 kilobase pairs to increase expression of the Epstein-Barr virus LMP gene

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12 December 2020

High levels of protein expression using different mammalian CMV promoters in several cell lines

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Precipitation of nucleic acids with polyethyleneimine and the chromatography of nucleic acids and proteins on immobilised polyethyleneimine

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12 December 2020

Transient transfection of CHO-K1-S using serum-free medium in suspension: a rapid mammalian protein expression system

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12 December 2020

Poly(ethylenimine) and its role in gene delivery

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Effects of pCIneo and pCEP4 expression vectors on transient and stable protein production in human and simian cell lines

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12 December 2020

Use of the human elongation factor 1 alpha promoter as a versatile and efficient expression system

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Functional analyses of the EBNA1 origin DNA binding protein of Epstein-Barr virus

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Nuclear proteins as gene-transfer vectors

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Powerful and versatile enhancer-promoter unit for mammalian expression vectors

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12 December 2020

Large-scale transient transfection of serum-free suspension-growing HEK293 EBNA1 cells: peptone additives improve cell growth and transfection efficiency

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Epstein-Barr virus-derived vectors for transient and stable expression of recombinant proteins.

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12 December 2020

Overcoming the nuclear barrier: cell cycle independent nonviral gene transfer with linear polyethylenimine or electroporation

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12 December 2020

Current issues in adeno-associated viral vector production

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12 December 2020

Intracellular route and transcriptional competence of polyethylenimine-DNA complexes

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12 December 2020

Epi-CHO, an episomal expression system for recombinant protein production in CHO cells

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12 December 2020

Highly efficient, large volume flow electroporation

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12 December 2020

Purification and characterization of a recombinant G-protein-coupled receptor, Saccharomyces cerevisiae Ste2p, transiently expressed in HEK293 EBNA1 cells

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Transient gene expression: recombinant protein production with suspension-adapted HEK293-EBNA cells

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12 December 2020

Helper activity for gene expression, a novel function of the SV40 enhancer.

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The linking regions of EBNA1 are essential for its support of replication and transcription

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12 December 2020

The human cytomegalovirus major immediate early promoter can be trans-activated by adenovirus early proteins

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12 December 2020

Small-scale bioreactor system for process development and optimization

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Transient-expression technologies, their application and scale-up: 5-HT3 serotonin receptor case study

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Recent advances in rational gene transfer vector design based on poly(ethylene imine) and its derivatives

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12 December 2020

Efficient transfection of DNA by mixing cells in suspension with calcium phosphate

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Role of endocytosis in the transfection of L929 fibroblasts by polyethylenimine/DNA complexes

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12 December 2020

Transfer and expression of foreign genes in mammalian cells

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Long-term replication of Epstein-Barr virus-derived episomal vectors in the rodent cells

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12 December 2020

Tracking the intracellular path of poly(ethylenimine)/DNA complexes for gene delivery

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12 December 2020

Metabolic instability of plasmid DNA in the cytosol: a potential barrier to gene transfer

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12 December 2020

Establishment of a human somatic hybrid cell line for recombinant protein production

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12 December 2020

Translation by the adenovirus tripartite leader: elements which determine independence from cap-binding protein complex

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12 December 2020

State-of-the-art of the production of retroviral vectors

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12 December 2020

Transient production of retroviral- and lentiviral-based vectors for the transduction of Mammalian cells

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12 December 2020

Calcium-phosphate mediated DNA transfer into HEK-293 cells in suspension: control of physicochemical parameters allows transfection in stirred media. Transfection and protein expression in mammalian cells

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12 December 2020

Analysis of mutation in human cells by using an Epstein-Barr virus shuttle system

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12 December 2020

Large-scale transient transfection of mammalian cells: a newly emerging attractive option for recombinant protein production

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12 December 2020

Utilization of the beta and gamma chains of the IL-2 receptor by the novel cytokine IL-15

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12 December 2020

A novel IL-1 receptor, cloned from B cells by mammalian expression, is expressed in many cell types

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12 December 2020

Different behavior of branched and linear polyethylenimine for gene delivery in vitro and in vivo

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12 December 2020

Large-scale transient expression of therapeutic proteins in mammalian cells

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12 December 2020

Calfection: a novel gene transfer method for suspension cells.

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12 December 2020

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

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12 December 2020

trans activation of an Epstein-Barr viral transcriptional enhancer by the Epstein-Barr viral nuclear antigen 1.

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12 December 2020

Intervening sequences increase efficiency of RNA 3' processing and accumulation of cytoplasmic RNA

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12 December 2020

Intracellular distribution of DNA internalized through calcium phosphate precipitation

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12 December 2020

Plasmid DNA purification

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12 December 2020

InsectDirect System: rapid, high-level protein expression and purification from insect cells

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12 December 2020

A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine

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12 December 2020

Non-GMP plasmid production for transient transfection in bioreactors

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12 December 2020

Up to 100-fold increase of apparent gene expression in the presence of Epstein-Barr virus oriP sequences and EBNA1: implications of the nuclear import of plasmids

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12 December 2020

Repression of simian virus 40 early transcription by viral DNA replication in human 293 cells

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12 December 2020

Epstein-Barr virus vectors for gene expression and transfer

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12 December 2020

Transient production of recombinant proteins by Chinese hamster ovary cells using polyethyleneimine/DNA complexes in combination with microtubule disrupting anti-mitotic agents

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12 December 2020

Versatile expression system for rapid and stable production of recombinant proteins

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12 December 2020

Polyethylenimine but not cationic lipids promotes transgene delivery to the nucleus in mammalian cells

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12 December 2020

A very strong enhancer is located upstream of an immediate early gene of human cytomegalovirus.

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12 December 2020

High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells

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12 December 2020

Biology of cell surface heparan sulfate proteoglycans

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12 December 2020

Production methods for gene transfer vectors based on adeno-associated virus serotypes

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12 December 2020

Inducing single-cell suspension of BTI-TN5B1-4 insect cells: I. The use of sulfated polyanions to prevent cell aggregation and enhance recombinant protein production

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12 December 2020

Identification of the components necessary for adenovirus translational control and their utilization in cDNA expression vectors

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12 December 2020

Serum-free suspension large-scale transient transfection of CHO cells in WAVE bioreactors.

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12 December 2020

Transfecting mammalian cells: optimization of critical parameters affecting calcium-phosphate precipitate formation

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12 December 2020

The human elongation factor 1 alpha (EF-1 alpha) first intron highly enhances expression of foreign genes from the murine cytomegalovirus promoter

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12 December 2020

Dioleoylmelittin as a novel serum-insensitive reagent for efficient transfection of mammalian cells

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12 December 2020

The plasmid replicon of EBV consists of multiple cis-acting elements that facilitate DNA synthesis by the cell and a viral maintenance element

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12 December 2020

Ca2+-sensitive cytosolic nucleases prevent efficient delivery to the nucleus of injected plasmids.

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12 December 2020

SV40 DNA transfection of cells in suspension: analysis of the efficiency of transcription and translation of T-antigen

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12 December 2020

In situ single cell observation by fluorescence resonance energy transfer reveals fast intra-cytoplasmic delivery and easy release of plasmid DNA complexed with linear polyethylenimine

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12 December 2020

Mechanisms of DNA uptake by mammalian cells: fate of exogenously added DNA monitored by the use of fluorescent dyes

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12 December 2020

The use of engineered E1A genes to transactivate the hCMV-MIE promoter in permanent CHO cell lines

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12 December 2020

Liposome-mediated transfection of mammalian cells.

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12 December 2020

Plasmid origin of replication of Epstein-Barr virus, oriP, does not limit replication in cis.

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12 December 2020

Hyperinducible gene expression from a metallothionein promoter containing additional metal-responsive elements

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12 December 2020

Size-dependent DNA mobility in cytoplasm and nucleus

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12 December 2020

Extraction of plasmid DNA using reactor scale alkaline lysis and selective precipitation for scalable transient transfection

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12 December 2020

Gene transfer in vitro and in vivo with Epstein-Barr virus-based episomal vector results in markedly high transient expression in rodent cells

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12 December 2020

Polyethylenimine-based non-viral gene delivery systems

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12 December 2020

Separation of the DNA replication, segregation, and transcriptional activation functions of Epstein-Barr nuclear antigen 1.

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12 December 2020

Regulated shedding of syndecan-1 and -4 ectodomains by thrombin and growth factor receptor activation.

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12 December 2020

Adenovirus tripartite leader sequence enhances translation of mRNAs late after infection

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12 December 2020

Evidence for the role of proteoglycans in cation-mediated gene transfer

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12 December 2020

Mechanisms of DNA entry into mammalian cells. II. Phagocytosis of calcium phosphate DNA co-precipitate visualized by electron microscopy

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12 December 2020

Production of lentiviral vectors.

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12 December 2020

Eukaryotic expression systems: a comparison

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12 December 2020

Transfer of high copy number plasmid into mammalian cells by calcium phosphate transfection

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12 December 2020

Characterization of commercially available and synthesized polyethylenimines for gene delivery

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12 December 2020

Characteristics of a human cell line transformed by DNA from human adenovirus type 5

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12 December 2020

Transient gene expression in suspension HEK-293 cells: application to large-scale protein production

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12 December 2020

The tripartite leader sequence of subgroup C adenovirus major late mRNAs can increase the efficiency of mRNA export.

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12 December 2020

A new technique for the assay of infectivity of human adenovirus 5 DNA

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12 December 2020

Prolonged and increased expression of soluble Fc receptors, IgG and a TCR-Ig fusion protein by transiently transfected adherent 293E cells

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12 December 2020

Exploring polyethylenimine-mediated DNA transfection and the proton sponge hypothesis

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12 December 2020

Polyoma and hamster papovavirus large T antigen-mediated replication of expression shuttle vectors in Chinese hamster ovary cells

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12 December 2020

Optimized galenics improve in vitro gene transfer with cationic molecules up to 1000-fold.

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12 December 2020

Identifiers

DOI

10.1385/MB:34:2:225

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Dimensions Publication ID

15 August 2017

1039028081

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