(Q36901557)

17 August 2017

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title

Beyond toothpicks: new methods for isolating mutant bacteria (English)

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17 August 2017

A James Link

1

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17 August 2017

Ki Jun Jeong

series ordinal

2

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17 August 2017

George Georgiou

series ordinal

3

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17 August 2017

language of work or name

English

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publication date

1 September 2007

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Nature Reviews Microbiology

17 August 2017

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17 August 2017

5

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17 August 2017

680-688

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17 August 2017

9

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https://scigraph.springernature.com/pub.10.1038/nrmicro1715

17 August 2017

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cites work

Just toothpicks and logic: how some labs succeed at solving complex problems

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https://api.crossref.org/works/10.1038%2FNRMICRO1715

The art and design of genetic screens: Escherichia coli

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Toothpicks, serendipity and the emergence of the Escherichia coli DnaK (Hsp70) and GroEL (Hsp60) chaperone machines

31 March 2020

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Chemical tools for biomolecular imaging

31 March 2020

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FACS-optimized mutants of the green fluorescent protein (GFP)

31 March 2020

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Analysis of large libraries of protein mutants using flow cytometry

31 March 2020

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Evolution of highly active enzymes by homology-independent recombination

31 March 2020

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https://api.crossref.org/works/10.1038%2FNRMICRO1715

The many faces of glutathione in bacteria

31 March 2020

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https://api.crossref.org/works/10.1038%2FNRMICRO1715

Genetically encoded fluorescent indicator for intracellular hydrogen peroxide

31 March 2020

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https://api.crossref.org/works/10.1038%2FNRMICRO1715

Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins

31 March 2020

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https://api.crossref.org/works/10.1038%2FNRMICRO1715

Noninvasive measurement of bacterial intracellular pH on a single-cell level with green fluorescent protein and fluorescence ratio imaging microscopy

31 March 2020

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pHluorin-based in vivo assay for hydrolase screening.

31 March 2020

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https://api.crossref.org/works/10.1038%2FNRMICRO1715

Direct visualization of red fluorescent lipoproteins indicates conservation of the membrane sorting rules in the family Enterobacteriaceae

31 March 2020

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https://api.crossref.org/works/10.1038%2FNRMICRO1715

Flow-cytometric detection of changes in the physiological state of E. coli expressing a heterologous membrane protein during carbon-limited fedbatch cultivation

31 March 2020

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An industrial application of multiparameter flow cytometry: assessment of cell physiological state and its application to the study of microbial fermentations.

31 March 2020

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A microfabricated fluorescence-activated cell sorter

31 March 2020

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An integrated microfabricated cell sorter

31 March 2020

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Integrating advanced functionality in a microfabricated high-throughput fluorescent-activated cell sorter.

31 March 2020

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https://api.crossref.org/works/10.1038%2FNRMICRO1715

Marker-specific sorting of rare cells using dielectrophoresis

31 March 2020

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Microfluidic library screening for mapping antibody epitopes

31 March 2020

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https://api.crossref.org/works/10.1038%2FNRMICRO1715

Microfluidic digital PCR enables multigene analysis of individual environmental bacteria

31 March 2020

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https://api.crossref.org/works/10.1038%2FNRMICRO1715

Green fluorescent protein as a marker for gene expression

31 March 2020

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A guide to choosing fluorescent proteins

31 March 2020

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A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications

31 March 2020

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Reducing the environmental sensitivity of yellow fluorescent protein. Mechanism and applications

31 March 2020

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Blue fluorescent proteins with enhanced brightness and photostability from a structurally targeted library

31 March 2020

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Fluorescent proteins from nonbioluminescent Anthozoa species

31 March 2020

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A monomeric red fluorescent protein

31 March 2020

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Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein

31 March 2020

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A comprehensive library of fluorescent transcriptional reporters for Escherichia coli

31 March 2020

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Just-in-time transcription program in metabolic pathways

31 March 2020

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https://api.crossref.org/works/10.1038%2FNRMICRO1715

Flow cytometry and bacterial pathogenesis

31 March 2020

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https://api.crossref.org/works/10.1038%2FNRMICRO1715

Fluorescence-based isolation of bacterial genes expressed within host cells

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNRMICRO1715

Genome-wide identification of plant-upregulated genes of Erwinia chrysanthemi 3937 using a GFP-based IVET leaf array

31 March 2020

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https://api.crossref.org/works/10.1038%2FNRMICRO1715

The obligate intracellular pathogen Chlamydia trachomatis targets host lipid droplets.

31 March 2020

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https://api.crossref.org/works/10.1038%2FNRMICRO1715

Cloning vectors and fluorescent proteins can significantly inhibit Salmonella enterica virulence in both epithelial cells and macrophages: implications for bacterial pathogenesis studies.

31 March 2020

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https://api.crossref.org/works/10.1038%2FNRMICRO1715

Systematic profiling of cellular phenotypes with spotted cell microarrays reveals mating-pheromone response genes

31 March 2020

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https://api.crossref.org/works/10.1038%2FNRMICRO1715

Global analysis of protein localization in budding yeast

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNRMICRO1715

Single-cell proteomic analysis of S. cerevisiae reveals the architecture of biological noise

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNRMICRO1715

Phage shock protein PspA of Escherichia coli relieves saturation of protein export via the Tat pathway

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNRMICRO1715

Genetic analysis of the twin arginine translocator secretion pathway in bacteria

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNRMICRO1715

Export pathway selectivity of Escherichia coli twin arginine translocation signal peptides

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNRMICRO1715

YpdC determines site-1 degradation in regulated intramembrane proteolysis of the RsiW anti-sigma factor of Bacillus subtilis.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNRMICRO1715

Evolutionary optimization of fluorescent proteins for intracellular FRET

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNRMICRO1715

Receptor sensitivity in bacterial chemotaxis

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNRMICRO1715

Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNRMICRO1715

Combinatorial marking of cells and organelles with reconstituted fluorescent proteins

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNRMICRO1715

Protein tagging and detection with engineered self-assembling fragments of green fluorescent protein

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNRMICRO1715

An improved mRFP1 adds red to bimolecular fluorescence complementation.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNRMICRO1715

Monitoring of conformational change in maltose binding protein using split green fluorescent protein

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNRMICRO1715

APEx 2-hybrid, a quantitative protein-protein interaction assay for antibody discovery and engineering

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNRMICRO1715

Function-based isolation of novel enzymes from a large library.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNRMICRO1715

Engineering of protease variants exhibiting high catalytic activity and exquisite substrate selectivity.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNRMICRO1715

An efficient system for the evolution of aminoacyl-tRNA synthetase specificity

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNRMICRO1715

Discovery of aminoacyl-tRNA synthetase activity through cell-surface display of noncanonical amino acids

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNRMICRO1715

Directed evolution of the site specificity of Cre recombinase

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNRMICRO1715

Screening for recombinant glutathione transferases active with monochlorobimane

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNRMICRO1715

High-throughput screening methodology for the directed evolution of glycosyltransferases

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNRMICRO1715

Ultra-high-throughput screening based on cell-surface display and fluorescence-activated cell sorting for the identification of novel biocatalysts

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNRMICRO1715

Man-made cell-like compartments for molecular evolution.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNRMICRO1715

High-throughput screening of enzyme libraries: thiolactonases evolved by fluorescence-activated sorting of single cells in emulsion compartments.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNRMICRO1715

Optimizing the affinity and specificity of proteins with molecular display

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNRMICRO1715

Display of heterologous proteins on the surface of microorganisms: from the screening of combinatorial libraries to live recombinant vaccines

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNRMICRO1715

Isolation of high-affinity ligand-binding proteins by periplasmic expression with cytometric screening (PECS).

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNRMICRO1715

Anchored periplasmic expression, a versatile technology for the isolation of high-affinity antibodies from Escherichia coli-expressed libraries

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNRMICRO1715

Directed evolution of antibody fragments with monovalent femtomolar antigen-binding affinity

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNRMICRO1715

Flow cytometric screening of cell-based libraries

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNRMICRO1715

Selecting and screening recombinant antibody libraries

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNRMICRO1715

Yeast display of antibody fragments: a discovery and characterization platform.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNRMICRO1715

Molecular evolution of antibody cross-reactivity for two subtypes of type A botulinum neurotoxin

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNRMICRO1715

Selection of functional T cell receptor mutants from a yeast surface-display library

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNRMICRO1715

Fine epitope mapping of anti-epidermal growth factor receptor antibodies through random mutagenesis and yeast surface display

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNRMICRO1715

Directed evolution of a single-chain class II MHC product by yeast display

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNRMICRO1715

Directed evolution of soluble single-chain human class II MHC molecules

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNRMICRO1715

Selection of peptide entry motifs by bacterial surface display

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNRMICRO1715

Isolation of cell specific peptide ligands using fluorescent bacterial display libraries

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNRMICRO1715

Mining a yeast library for brain endothelial cell-binding antibodies

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNRMICRO1715

Rapid isolation of high-affinity protein binding peptides using bacterial display

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNRMICRO1715

Bacterial display using circularly permuted outer membrane protein OmpX yields high affinity peptide ligands

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNRMICRO1715

Protease specificity determination by using cellular libraries of peptide substrates (CLiPS).

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNRMICRO1715

31 March 2020

Identifiers

DOI

10.1038/NRMICRO1715

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Dimensions Publication ID

17 August 2017

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