(Q36943800)

17 August 2017

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title

Production of active eukaryotic proteins through bacterial expression systems: a review of the existing biotechnology strategies (English)

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17 August 2017

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Sudhir Sahdev

1

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17 August 2017

Sunil K Khattar

series ordinal

2

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17 August 2017

Kulvinder Singh Saini

series ordinal

3

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17 August 2017

publication date

12 September 2007

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Molecular and Cellular Biochemistry

17 August 2017

published in

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17 August 2017

volume

307

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17 August 2017

issue

1-2

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17 August 2017

page(s)

249-264

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https://scigraph.springernature.com/pub.10.1007/s11010-007-9603-6

17 August 2017

exact match

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cites work

Efficient production of the C-terminal domain of secretory leukoprotease inhibitor as a thrombin-cleavable fusion protein in Escherichia coli.

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12 December 2020

Significance of codon usage and irregularities of rare codon distribution in genes for expression of BspLU11III methyltransferases.

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12 December 2020

Refolding of a recombinant full-length non-structural (NS3) protein from hepatitis C virus by chromatographic procedures

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12 December 2020

A novel sequential procedure to enhance the renaturation of recombinant protein from Escherichia coli inclusion bodies

1 reference

12 December 2020

Efficient folding of proteins with multiple disulfide bonds in the Escherichia coli cytoplasm

1 reference

12 December 2020

ClpB cooperates with DnaK, DnaJ, and GrpE in suppressing protein aggregation. A novel multi-chaperone system from Escherichia coli

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12 December 2020

Solubilization of active green fluorescent protein from insoluble particles by guanidine and arginine

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12 December 2020

Influence of protein disulfide isomerase (PDI) on antibody folding in vitro

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12 December 2020

Statistical experimental design for bioprocess modeling and optimization analysis: repeated-measures method for dynamic biotechnology process.

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12 December 2020

Codon usage tabulated from international DNA sequence databases: status for the year 2000

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12 December 2020

Role of Hsp70 (DnaK-DnaJ-GrpE) and Hsp100 (ClpA and ClpB) chaperones in refolding and increased thermal stability of bacterial luciferases in Escherichia coli cells

1 reference

12 December 2020

Conserved role of the linker alpha-helix of the bacterial disulfide isomerase DsbC in the avoidance of misoxidation by DsbB.

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12 December 2020

Analysis for residual host cell proteins and DNA in process streams of a recombinant protein product expressed in Escherichia coli cells

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12 December 2020

Expression and purification of active PKB kinase from Escherichia coli

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12 December 2020

Binding of non-native protein to Hsp25 during heat shock creates a reservoir of folding intermediates for reactivation.

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12 December 2020

ClpB and HtpG facilitate de novo protein folding in stressed Escherichia coli cells

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12 December 2020

The Escherichia coli small heat-shock proteins IbpA and IbpB prevent the aggregation of endogenous proteins denatured in vivo during extreme heat shock

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12 December 2020

The likelihood of aggregation during protein renaturation can be assessed using the second virial coefficient

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12 December 2020

Co-variation of tRNA abundance and codon usage in Escherichia coli at different growth rates.

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12 December 2020

A periplasmic protein (Skp) of Escherichia coli selectively binds a class of outer membrane proteins.

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12 December 2020

Two-step metal affinity purification of double-tagged (NusA-His6) fusion proteins.

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12 December 2020

Production of correctly folded Fab antibody fragment in the cytoplasm of Escherichia coli trxB gor mutants via the coexpression of molecular chaperones

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12 December 2020

Escherichia coli small heat shock proteins, IbpA and IbpB, protect enzymes from inactivation by heat and oxidants

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12 December 2020

Evidence for non-enzymatic glycosylation of Escherichia coli chromosomal DNA.

1 reference

12 December 2020

Refolding of alpha 1-antitrypsin expressed as inclusion bodies in Escherichia coli: characterization of aggregation

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12 December 2020

Improvement of posttranslational bottlenecks in the production of penicillin amidase in recombinant Escherichia coli strains

1 reference

12 December 2020

Accelerated on-column lysine derivatization and cysteine methylation by imidazole reaction in a deuterated environment for enhanced product ion analysis

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12 December 2020

The small heat-shock protein IbpB from Escherichia coli stabilizes stress-denatured proteins for subsequent refolding by a multichaperone network

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12 December 2020

The SecA subunit of Escherichia coli preprotein translocase is exposed to the periplasm

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12 December 2020

Characterization of des-(741-1668)-factor VIII, a single-chain factor VIII variant with a fusion site susceptible to proteolysis by thrombin and factor Xa.

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12 December 2020

Reconstitution of lactic dehydrogenase. Noncovalent aggregation vs. reactivation. 2. Reactivation of irreversibly denatured aggregates

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12 December 2020

Optimizing the expression of a monoclonal antibody fragment under the transcriptional control of the Escherichia coli lac promoter

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12 December 2020

Refolding and Characterization of Rat Liver Methionine Adenosyltransferase from Escherichia coli Inclusion Bodies

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12 December 2020

Identification of factors impeding the production of a single-chain antibody fragment in Escherichia coliby comparing in vivo and in vitro expression

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12 December 2020

Use of bacteriophage T7 lysozyme to improve an inducible T7 expression system

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12 December 2020

Transcription of cspA, the gene for the major cold-shock protein of Escherichia coli, is negatively regulated at 37 degrees C by the 5'-untranslated region of its mRNA

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12 December 2020

New components of protein folding in extracytoplasmic compartments of Escherichia coli SurA, FkpA and Skp/OmpH.

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12 December 2020

Method for enhancing solubility of the expressed recombinant proteins in Escherichia coli

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12 December 2020

Extended -10 motif is critical for activity of the cspA promoter but does not contribute to low-temperature transcription

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12 December 2020

Characterization of fluorescent chimeras of cholera toxin and Escherichia coli heat-labile enterotoxins produced by use of the twin arginine translocation system

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12 December 2020

Nonenzymic reactivation of reduced bovine pancreatic ribonuclease by air oxidation and by glutathione oxidoreduction buffers

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12 December 2020

Inclusion body purification and protein refolding using microfiltration and size exclusion chromatography.

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12 December 2020

Highly-efficient purification of native polyhistidine-tagged proteins by multivalent NTA-modified magnetic nanoparticles

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12 December 2020

Refolding of protein inclusion bodies directly from E. coli homogenate using expanded bed adsorption chromatography

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12 December 2020

Novel protein purification system utilizing an N-terminal fusion protein and a caspase-3 cleavable linker

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12 December 2020

The relative protein disulphide isomerase (PDI) activities of gonadotrophins, thioredoxin and PDI

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12 December 2020

Secretion and export of IGF-1 in Escherichia coli strain JM101.

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12 December 2020

Increased solubility of integrin betaA domain using maltose-binding protein as a fusion tag

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12 December 2020

Expression of soluble, biologically active recombinant human endostatin in Escherichia coli

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12 December 2020

Different glycosyltransferases are differentially processed for secretion, dimerization, and autoglycosylation.

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12 December 2020

A temperature-dependent switch from chaperone to protease in a widely conserved heat shock protein.

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12 December 2020

Effects of aromatic thiols on thiol-disulfide interchange reactions that occur during protein folding

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12 December 2020

Osmotic shock: a mechanosensitive channel blocker can prevent release of cytoplasmic but not periplasmic proteins.

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12 December 2020

A method for increasing the yield of properly folded recombinant fusion proteins: single-chain immunotoxins from renaturation of bacterial inclusion bodies

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12 December 2020

Protein refolding at high concentration using size-exclusion chromatography

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12 December 2020

Effect of oxygen limitation and medium composition on Escherichia coli fermentation in shake-flask cultures

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12 December 2020

Formation of active inclusion bodies in the periplasm of Escherichia coli.

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12 December 2020

Correct disulfide pairing and efficient refolding of detergent-solubilized single-chain Fv proteins from bacterial inclusion bodies

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12 December 2020

An in vivo pathway for disulfide bond isomerization in Escherichia coli

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12 December 2020

A new Escherichia coli gene, dsbG, encodes a periplasmic protein involved in disulphide bond formation, required for recycling DsbA/DsbB and DsbC redox proteins

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12 December 2020

Expression of recombinant human phenylalanine hydroxylase as fusion protein in Escherichia coli circumvents proteolytic degradation by host cell proteases. Isolation and characterization of the wild-type enzyme

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12 December 2020

Disulfide-dependent folding and export of Escherichia coli DsbC.

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12 December 2020

A new heat-shock gene, ppiD, encodes a peptidyl-prolyl isomerase required for folding of outer membrane proteins in Escherichia coli

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12 December 2020

The conformational quality of insoluble recombinant proteins is enhanced at low growth temperatures.

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12 December 2020

Effective renaturation of reduced lysozyme by gentle removal of urea

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12 December 2020

Cytoplasmic overexpression, folding, and processing of penicillin acylase precursor in Escherichia coli.

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12 December 2020

Secretion-dependent proteolysis of heterologous protein by recombinant Escherichia coli is connected to an increased activity of the energy-generating dissimilatory pathway

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12 December 2020

Improved refolding of an immobilized fusion protein

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12 December 2020

In vivo and in vitro protein solubility assays using split GFP.

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12 December 2020

Gene expression analysis of Escherichia coli grown in miniaturized bioreactor platforms for high-throughput analysis of growth and genomic data

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12 December 2020

Overexpression of protein disulfide isomerase DsbC stabilizes multiple-disulfide-bonded recombinant protein produced and transported to the periplasm in Escherichia coli

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12 December 2020

Protein production in Escherichia coli for structural studies by X-ray crystallography

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12 December 2020

Oxidation of alkaline immobiline buffers for isoelectric focusing in immobilized pH gradients.

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12 December 2020

The periplasmic Escherichia coli peptidylprolyl cis,trans-isomerase FkpA. I. Increased functional expression of antibody fragments with and without cis-prolines.

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12 December 2020

Chaperonins govern growth of Escherichia coli at low temperatures.

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12 December 2020

Effects of codon usage versus putative 5'-mRNA structure on the expression of Fusarium solani cutinase in the Escherichia coli cytoplasm

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12 December 2020

From gene to protein: a review of new and enabling technologies for multi-parallel protein expression

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12 December 2020

Dual gradient ion-exchange chromatography improved refolding yield of lysozyme

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12 December 2020

Correlation between the abundance of Escherichia coli transfer RNAs and the occurrence of the respective codons in its protein genes

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12 December 2020

Synthesis of Macromolecules by Escherichia coli near the Minimal Temperature for Growth

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12 December 2020

Expression of porcine circovirus 2 ORF2 gene requires codon optimized E. coli cells

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12 December 2020

Trigger Factor and DnaK possess overlapping substrate pools and binding specificities

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12 December 2020

The nascent polypeptide-associated complex modulates interactions between the signal recognition particle and the ribosome

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12 December 2020

Genetic design for facilitated production and recovery of recombinant proteins in Escherichia coli.

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12 December 2020

A novel fusion protein system for the production of native human pepsinogen in the bacterial periplasm.

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12 December 2020

Optimized removal of soluble host cell proteins for the recovery of met-human growth hormone inclusion bodies from Escherichia coli cell lysate using crossflow microfiltration.

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12 December 2020

Catalytic activity and chaperone function of human protein-disulfide isomerase are required for the efficient refolding of proinsulin

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12 December 2020

Involvement of SecB, a chaperone, in the export of ribose-binding protein

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12 December 2020

A family of E. coli expression vectors for laboratory scale and high throughput soluble protein production

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12 December 2020

Chaperone function of FkpA, a heat shock prolyl isomerase, in the periplasm of Escherichia coli

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12 December 2020

Expression of aggregation-prone recombinant proteins at low temperatures: a comparative study of the Escherichia coli cspA and tac promoter systems

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12 December 2020

Skp, a molecular chaperone of gram-negative bacteria, is required for the formation of soluble periplasmic intermediates of outer membrane proteins

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12 December 2020

Presence of the cofactor speeds up folding of Desulfovibrio desulfuricans flavodoxin

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12 December 2020

A simplified procedure for the reduction and alkylation of cysteine residues in proteins prior to proteolytic digestion and mass spectral analysis

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12 December 2020

On-column refolding purification and characterization of recombinant human interferon-lambda1 produced in Escherichia coli.

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12 December 2020

Optimized procedure for renaturation of recombinant human bone morphogenetic protein-2 at high protein concentration

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12 December 2020

Bacterially expressed recombinant p68 RNA helicase is phosphorylated on serine, threonine, and tyrosine residues

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12 December 2020

Reversible protection of disulfide bonds followed by oxidative folding render recombinant hCGbeta highly immunogenic

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12 December 2020

Oxidative renaturation of hen egg-white lysozyme. Folding vs aggregation

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12 December 2020

GTP binding and hydrolysis by the signal recognition particle during initiation of protein translocation

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12 December 2020

Regulation by the ribosome of the GTPase of the signal-recognition particle during protein targeting

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12 December 2020

Continuous matrix-assisted refolding of proteins

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12 December 2020

Urea gradient size-exclusion chromatography enhanced the yield of lysozyme refolding

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12 December 2020

Membrane topology of the multidrug transporter MdfA: complementary gene fusion studies reveal a nonessential C-terminal domain

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12 December 2020

Enhanced soluble production of biologically active recombinant human p38 mitogen-activated-protein kinase (MAPK) in Escherichia coli.

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12 December 2020

Consecutive low-usage leucine codons block translation only when near the 5' end of a message in Escherichia coli.

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12 December 2020

Inhibition of aggregation side reactions during in vitro protein folding

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12 December 2020

Fermentation process for tetrameric human collagen prolyl 4-hydroxylase in Escherichia coli: improvement by gene optimisation of the PDI/beta subunit and repeated addition of the inducer anhydrotetracycline.

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12 December 2020

Cyanate formation in solutions of urea. I. Calculation of cyanate concentrations at different temperature and pH.

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12 December 2020

Production of leptin in Escherichia coli: a comparison of methods

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12 December 2020

Eukaryotic protein disulfide isomerase complements Escherichia coli dsbA mutants and increases the yield of a heterologous secreted protein with disulfide bonds.

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12 December 2020

Bacterial single-stranded DNA-binding proteins are phosphorylated on tyrosine

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12 December 2020

Co-translational protein targeting catalyzed by the Escherichia coli signal recognition particle and its receptor

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12 December 2020

Export and secretion of overproduced OmpA-beta-lactamase in Escherichia coli.

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12 December 2020

Controlling basal expression in an inducible T7 expression system by blocking the target T7 promoter with lac repressor

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12 December 2020

Refolding, structural transition and spermatozoa-binding of recombinant bonnet monkey (Macaca radiata) zona pellucida glycoprotein-C expressed in Escherichia coli

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12 December 2020

Solubilization of growth hormone and other recombinant proteins from Escherichia coli inclusion bodies by using a cationic surfactant

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12 December 2020

RNA-mediated interaction between the peptide-binding and GTPase domains of the signal recognition particle.

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12 December 2020

Removing the regulatory N-terminal domain of cardiac troponin I diminishes incompatibility during bacterial expression

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12 December 2020

High-level expression of recombinant genes in Escherichia coli is dependent on the availability of the dnaY gene product

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12 December 2020

Renaturation of Escherichia coli-derived recombinant human macrophage colony-stimulating factor

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12 December 2020

Renaturation of heterodimeric platelet-derived growth factor from inclusion bodies of recombinant Escherichia coli using size-exclusion chromatography

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12 December 2020

Refolding and recovery of recombinant human matrix metalloproteinase 7 (matrilysin) from inclusion bodies expressed by Escherichia coli

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12 December 2020

A hybrid plasmid for expression of toxic malarial proteins in Escherichia coli

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12 December 2020

High activity of inclusion bodies formed in Escherichia coli overproducing Clostridium thermocellum endoglucanase D.

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12 December 2020

New fusion protein systems designed to give soluble expression in Escherichia coli

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12 December 2020

Expression, single-step purification, and matrix-assisted refolding of a maize cytokinin glucoside-specific beta-glucosidase

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12 December 2020

Procedure for refolding and purification of recombinant proteins from Escherichia coli inclusion bodies using a strong anion exchanger.

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12 December 2020

Optimization of Inclusion Body Solubilization and Renaturation of Recombinant Human Growth Hormone from Escherichia coli

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12 December 2020

Interactions between protein disulphide isomerase and peptides

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12 December 2020

Overexpression of trigger factor prevents aggregation of recombinant proteins in Escherichia coli

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12 December 2020

Evidence for non-enzymatic glycosylation in Escherichia coli

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12 December 2020

Strategies to maximize heterologous protein expression in Escherichia coli with minimal cost

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12 December 2020

Effect of N-terminal solubility enhancing fusion proteins on yield of purified target protein.

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12 December 2020

Refolding and purification of Zymomonas mobilis levansucrase produced as inclusion bodies in fed-batch culture of recombinant Escherichia coli

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12 December 2020

Matrix-Assisted Refolding of Single-Chain Fv– Cellulose Binding Domain Fusion Proteins

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12 December 2020

Recombinant human TIMP-3 from Escherichia coli: synthesis, refolding, physico-chemical and functional insights.

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12 December 2020

Refolding of denatured/reduced lysozyme at high concentration with diafiltration

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12 December 2020

Controlled intracellular processing of fusion proteins by TEV protease

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12 December 2020

Functional coexpression of serine protein kinase SRPK1 and its substrate ASF/SF2 in Escherichia coli

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12 December 2020

Solubilization of recombinant ovine growth hormone with retention of native-like secondary structure and its refolding from the inclusion bodies of Escherichia coli

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12 December 2020

A favorable solubility partner for the recombinant expression of streptavidin

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12 December 2020

Engineered DsbC chimeras catalyze both protein oxidation and disulfide-bond isomerization in Escherichia coli: Reconciling two competing pathways

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12 December 2020

Enhancement of recombinant soluble dengue virus 2 envelope domain III protein production in Escherichia coli trxB and gor double mutant

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12 December 2020

Improvement of productivity of active horseradish peroxidase in Escherichia coli by coexpression of Dsb proteins.

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12 December 2020

Expression of a recombinant Toxoplasma gondii ROP2 fragment as a fusion protein in bacteria circumvents insolubility and proteolytic degradation

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12 December 2020

Expression of Chinese hamster cAMP-dependent protein kinase in Escherichia coli results in growth inhibition of bacterial cells: a model system for the rapid screening of mutant type I regulatory subunits

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12 December 2020

Improved protein refolding using hollow-fibre membrane dialysis

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12 December 2020

Comparative expression study to increase the solubility of cold adapted Vibrio proteins in Escherichia coli.

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12 December 2020

Molecular chaperones facilitate the soluble expression of N-acyl-D-amino acid amidohydrolases in Escherichia coli.

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12 December 2020

One-step purification of chimeric green fluorescent protein providing metal-binding avidity and protease recognition sequence

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12 December 2020

Matrix-assisted in vitro refolding of Pseudomonas aeruginosa class II polyhydroxyalkanoate synthase from inclusion bodies produced in recombinant Escherichia coli

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12 December 2020

Changes in the periplasmic linker and in the expression level affect the activity of ToxR and lambda-ToxR fusion proteins in Escherichia coli

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12 December 2020

High-level expression, purification, and renaturation of recombinant murine interleukin-2 from Escherichia coli

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12 December 2020

P22 tailspike trimer assembly is governed by interchain redox associations.

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12 December 2020

Expression and purification of bioactive high-purity mouse monokine induced by IFN-gamma in Escherichia coli.

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12 December 2020

Effects of consecutive AGG codons on translation in Escherichia coli, demonstrated with a versatile codon test system

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12 December 2020

Expression and refolding of recombinant human alpha-tocopherol transfer protein capable of specific alpha-tocopherol binding

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12 December 2020

The twin-arginine translocation system and its capability for protein secretion in biotechnological protein production

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12 December 2020

Inclusion body anatomy and functioning of chaperone-mediated in vivo inclusion body disassembly during high-level recombinant protein production in Escherichia coli

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12 December 2020

Temperature effect on inclusion body formation and stress response in the periplasm of Escherichia coli

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12 December 2020

Effects of rare codon clusters on high-level expression of heterologous proteins in Escherichia coli

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12 December 2020

Membrane targeting of a folded and cofactor-containing protein

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12 December 2020

A new strategy for the synthesis of glycoproteins.

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12 December 2020

Optimisation of production of a domoic acid-binding scFv antibody fragment in Escherichia coli using molecular chaperones and functional immobilisation on a mesoporous silicate support.

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12 December 2020

High-level periplasmic expression in Escherichia coli using a eukaryotic signal peptide: importance of codon usage at the 5' end of the coding sequence

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12 December 2020

Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused

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12 December 2020

Preparation of hydrophobic interaction chromatographic packings based on monodisperse poly(glycidylmethacrylate-co-ethylenedimethacrylate) beads and their application

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12 December 2020

Solubilization and renaturation of overexpressed aggregates of mutant tryptophan synthase alpha-subunits

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12 December 2020

Refolding proteins by gel filtration chromatography.

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12 December 2020

Overproduction of bacterial protein disulfide isomerase (DsbC) and its modulator (DsbD) markedly enhances periplasmic production of human nerve growth factor in Escherichia coli

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12 December 2020

Purification and characterization of recombinant extracellular domain of human HER2 from Escherichia coli.

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12 December 2020

SRP-dependent co-translational targeting and SecA-dependent translocation analyzed as individual steps in the export of a bacterial protein

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12 December 2020

Thioredoxin fusions increase folding of single chain Fv antibodies in the cytoplasm of Escherichia coli: evidence that chaperone activity is the prime effect of thioredoxin

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12 December 2020

Production of recombinant thermostable proteins expressed in Escherichia coli: completion of protein synthesis is the bottleneck

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12 December 2020

Phosphorylation of Isocitrate Dehydrogenase of Escherichia coli

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12 December 2020

Considerations of sample application and elution during size-exclusion chromatography-based protein refolding.

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12 December 2020

Secretion of active truncated CD4 into Escherichia coli periplasm

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12 December 2020

FRET-based in vivo screening for protein folding and increased protein stability

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12 December 2020

The reductive enzyme thioredoxin 1 acts as an oxidant when it is exported to the Escherichia coli periplasm

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12 December 2020

Chromatographic methods for the isolation of, and refolding of proteins from, Escherichia coli inclusion bodies

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12 December 2020

Improved conditions for production of recombinant plant sesquiterpene synthases in Escherichia coli.

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12 December 2020

A kinetic study of the competition between renaturation and aggregation during the refolding of denatured-reduced egg white lysozyme.

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12 December 2020

Errors of heterologous protein expression

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12 December 2020

Theoretical analysis of amino acid-producing Escherichia coli using a stoichiometric model and multivariate linear regression.

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12 December 2020

Evaluation of refolding conditions for a recombinant human interleukin-3 variant (daniplestim)

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12 December 2020

Effects of solutes on solubilization and refolding of proteins from inclusion bodies with high hydrostatic pressure

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12 December 2020

The oxidative folding of proteins by disulfide plus thiol does not correlate with redox potential

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12 December 2020

Aromatic thiol pKa effects on the folding rate of a disulfide containing protein.

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12 December 2020

Refolding and activation of human prorenin expressed in Escherichia coli: application of recombinant human renin for inhibitor screening

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12 December 2020

Targeting and translocation of two lipoproteins in Escherichia coli via the SRP/Sec/YidC pathway.

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12 December 2020

Codon usage and gene expression level in Dictyostelium discoideum: highly expressed genes do 'prefer' optimal codons

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12 December 2020

Over-expression of Escherichia coli F1F(o)-ATPase subunit a is inhibited by instability of the uncB gene transcript.

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12 December 2020

10.1007/S11010-007-9603-6

Identifiers

DOI

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17 August 2017

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