(Q37604933)

21 August 2017

Production of isotopically labeled heterologous proteins in non-E. coli prokaryotic and eukaryotic cells (English)

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21 August 2017

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Hideo Takahashi

1

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PubMed ID

19787297

21 August 2017

Ichio Shimada

series ordinal

2

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21 August 2017

29 September 2009

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Journal of Biomolecular NMR

21 August 2017

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21 August 2017

46

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21 August 2017

3-10

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21 August 2017

1

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https://scigraph.springernature.com/pub.10.1007/s10858-009-9377-0

21 August 2017

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cites work

Functional characterization of the two alcohol oxidase genes from the yeast Pichia pastoris

1 reference

Crossref

https://api.crossref.org/works/10.1007%2FS10858-009-9377-0

An economical method for (15)N/(13)C isotopic labeling of proteins expressed in Pichia pastoris

21 January 2018

1 reference

Crossref

https://api.crossref.org/works/10.1007%2FS10858-009-9377-0

Solution NMR spectroscopy of [alpha -15N]lysine-labeled rhodopsin: The single peak observed in both conventional and TROSY-type HSQC spectra is ascribed to Lys-339 in the carboxyl-terminal peptide sequence

21 January 2018

1 reference

12 December 2020

Enhancement of soluble protein expression through the use of fusion tags

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12 December 2020

Recombinant protein expression in Pichia pastoris

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12 December 2020

Large-scale expression and purification of the major HIV-1 coreceptor CCR5 and characterization of its interaction with RANTES.

1 reference

12 December 2020

Expression of human chorionic gonadotropin uniformly labeled with NMR isotopes in Chinese hamster ovary cells: an advance toward rapid determination of glycoprotein structures

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12 December 2020

How physiological and cultural conditions influence heterologous protein production in Kluyveromyces lactis.

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12 December 2020

Amino acid-selective isotope labeling of proteins for nuclear magnetic resonance study: proteins secreted by Brevibacillus choshinensis.

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12 December 2020

Structural basis of the collagen-binding mode of discoidin domain receptor 2

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12 December 2020

Isotope labeling of mammalian GPCRs in HEK293 cells and characterization of the C-terminus of bovine rhodopsin by high resolution liquid NMR spectroscopy

1 reference

12 December 2020

Efficient uniform isotope labeling of Abl kinase expressed in Baculovirus-infected insect cells

1 reference

12 December 2020

Nuclear magnetic resonance study of antibodies: a multinuclear approach

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12 December 2020

A novel NMR method for determining the interfaces of large protein-protein complexes

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12 December 2020

Structural genomics on membrane proteins: comparison of more than 100 GPCRs in 3 expression systems.

1 reference

12 December 2020

Differential dynamics in the G protein-coupled receptor rhodopsin revealed by solution NMR.

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12 December 2020

Stable isotope labeling of protein by Kluyveromyces lactis for NMR study

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12 December 2020

Production of large quantities of isotopically labeled protein in Pichia pastoris by fermentation

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12 December 2020

A novel medium for expression of proteins selectively labeled with 15N-amino acids in Spodoptera frugiperda (Sf9) insect cells

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12 December 2020

High expression with Corynebacterium glutamicum for nuclear magnetic resonance sample preparation

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12 December 2020

Solution conformations and dynamics of ABL kinase-inhibitor complexes determined by NMR substantiate the different binding modes of imatinib/nilotinib and dasatinib

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12 December 2020

Heterologous expression of a deuterated membrane-integrated receptor and partial deuteration in methylotrophic yeasts.

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12 December 2020

Expression of deuterium-isotope-labelled protein in the yeast pichia pastoris for NMR studies

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12 December 2020

Biosynthesis of isotopically labeled gramicidins and tyrocidins by Bacillus brevis.

1 reference

12 December 2020

High-level secretion of heterologous proteins by Bacillus brevis

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12 December 2020

Backbone 1H, 13C, and 15N resonance assignments of the anti-dansyl antibody Fv fragment

1 reference

12 December 2020

High-level secretion and very efficient isotopic labeling of tick anticoagulant peptide (TAP) expressed in the methylotrophic yeast, Pichia pastoris.

1 reference

12 December 2020

Cold-shock induced high-yield protein production in Escherichia coli.

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12 December 2020

Preparative expression of secreted proteins in bacteria: status report and future prospects

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12 December 2020

Kluyveromyces lactis LAC4 promoter variants that lack function in bacteria but retain full function in K. lactis

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12 December 2020

A practical method for uniform isotopic labeling of recombinant proteins in mammalian cells

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12 December 2020

Solid state 15N NMR evidence for a complex Schiff base counterion in the visual G-protein-coupled receptor rhodopsin

1 reference

12 December 2020

15N isotope labeling of a protein secreted by Brevibacillus choshinensis for NMR study

1 reference

12 December 2020

Development of a high-level expression system for deuterium-labeled human serum albumin

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12 December 2020

Stable-isotope labeling using an inducible viral infection system in suspension-cultured plant cells

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12 December 2020

A simple, quick, and high-yield preparation of the human thromboxane A2 receptor in full size for structural studies

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12 December 2020

Select what you need: a comparative evaluation of the advantages and limitations of frequently used expression systems for foreign genes

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12 December 2020

Protein production and purification

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12 December 2020

Solution NMR spectroscopy beyond 25 kDa.

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12 December 2020

Preparation of amino-acid-type selective isotope labeling of protein expressed in Pichia pastoris.

1 reference

12 December 2020

Structural genomics for membrane proteins

1 reference

12 December 2020

Amino-acid-type selective isotope labeling of proteins expressed in Baculovirus-infected insect cells useful for NMR studies

1 reference

12 December 2020

Determination of the interface of a large protein complex by transferred cross-saturation measurements

1 reference

12 December 2020

10.1007/S10858-009-9377-0

Identifiers

DOI

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21 August 2017

1 reference

PubMed ID

19787297