(Q37660070)

21 August 2017

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title

Incorporation of unnatural amino acids for synthetic biology (English)

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21 August 2017

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Jin Kim Montclare

2

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author name string

Natalya Voloshchuk

series ordinal

1

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21 August 2017

publication date

4 September 2009

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21 August 2017

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21 August 2017

volume

6

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21 August 2017

issue

1

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21 August 2017

page(s)

65-80

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Synthetic biology: new engineering rules for an emerging discipline

21 August 2017

cites work

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Engineering life: building a fab for biology

21 January 2018

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Designing biological systems

21 January 2018

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Synthetic gene circuits: design with directed evolution

21 January 2018

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Modular approaches to expanding the functions of living matter

21 January 2018

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Accurate multiplex gene synthesis from programmable DNA microchips

21 January 2018

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Protein-mediated error correction for de novo DNA synthesis

21 January 2018

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A synthetic multicellular system for programmed pattern formation

21 January 2018

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Production of the antimalarial drug precursor artemisinic acid in engineered yeast

21 January 2018

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Dispersing biofilms with engineered enzymatic bacteriophage

21 January 2018

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Engineering the Genetic Code

21 January 2018

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Programming and engineering biological networks

21 January 2018

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Adding amino acids to the genetic repertoire

21 January 2018

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A chemical toolkit for proteins--an expanded genetic code

21 January 2018

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The incorporation of a photoisomerizable amino acid into proteins in E. coli

21 January 2018

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A genetically encoded infrared probe.

21 January 2018

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Site-specific incorporation of a redox-active amino acid into proteins

21 January 2018

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Biosynthesis of a highly stable coiled-coil protein containing hexafluoroleucine in an engineered bacterial host

21 January 2018

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Antimicrobial activity and protease stability of peptides containing fluorinated amino acids.

21 January 2018

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A synthetic oscillatory network of transcriptional regulators

21 January 2018

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Construction of a genetic toggle switch in Escherichia coli

21 January 2018

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Development of genetic circuitry exhibiting toggle switch or oscillatory behavior in Escherichia coli

21 January 2018

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Reprogramming control of an allosteric signaling switch through modular recombination

21 January 2018

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Engineering synthetic signaling proteins with ultrasensitive input/output control

21 January 2018

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Rewiring MAP kinase pathways using alternative scaffold assembly mechanisms

21 January 2018

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Integration of multiple signals through cooperative regulation of the N-WASP-Arp2/3 complex

21 January 2018

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Optimization of specificity in a cellular protein interaction network by negative selection

21 January 2018

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Rewiring cell signaling: the logic and plasticity of eukaryotic protein circuitry

21 January 2018

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Computational design of receptor and sensor proteins with novel functions

21 January 2018

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A synthetic gene-metabolic oscillator.

21 January 2018

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Artificial protein sensors

21 January 2018

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Computational systems biology

21 January 2018

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Gaining confidence in high-throughput protein interaction networks

21 January 2018

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Detection of functional modules from protein interaction networks.

21 January 2018

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Metabolic engineering of microorganisms for biofuels production: from bugs to synthetic biology to fuels

21 January 2018

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Negative autoregulation speeds the response times of transcription networks

21 January 2018

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Synthetic gene networks that count

21 January 2018

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Engineered bidirectional communication mediates a consensus in a microbial biofilm consortium

21 January 2018

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Using engineered scaffold interactions to reshape MAP kinase pathway signaling dynamics

21 January 2018

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Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels

21 January 2018

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Toward scalable parts families for predictable design of biological circuits

21 January 2018

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Selenocysteine incorporation machinery and the role of selenoproteins in development and health

21 January 2018

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The direct genetic encoding of pyrrolysine

21 January 2018

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Genetic code: introducing pyrrolysine

21 January 2018

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A general method for site-specific incorporation of unnatural amino acids into proteins.

21 January 2018

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Enzymatic aminoacylation of tRNA with unnatural amino acids

21 January 2018

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Addition of p-azido-L-phenylalanine to the genetic code of Escherichia coli

21 January 2018

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Improving nature's enzyme active site with genetically encoded unnatural amino acids

21 January 2018

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Site-specific incorporation of a (19)F-amino acid into proteins as an NMR probe for characterizing protein structure and reactivity.

21 January 2018

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Addition of the keto functional group to the genetic code of Escherichia coli.

21 January 2018

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The site-specific incorporation of p-iodo-L-phenylalanine into proteins for structure determination

21 January 2018

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A new strategy for the synthesis of glycoproteins.

21 January 2018

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Generation of a bacterium with a 21 amino acid genetic code

21 January 2018

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A general approach for the generation of orthogonal tRNAs.

21 January 2018

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An expanded eukaryotic genetic code

21 January 2018

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An efficient system for the evolution of aminoacyl-tRNA synthetase specificity

21 January 2018

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Addition of an alpha-hydroxy acid to the genetic code of bacteria.

21 January 2018

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Efficient incorporation of unnatural amino acids into proteins in Escherichia coli.

21 January 2018

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Site-specific incorporation of tryptophan analogues into recombinant proteins in bacterial cells

21 January 2018

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Design of a bacterial host for site-specific incorporation of p-bromophenylalanine into recombinant proteins.

21 January 2018

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One plasmid selection system for the rapid evolution of aminoacyl-tRNA synthetases

21 January 2018

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An improved system for the generation and analysis of mutant proteins containing unnatural amino acids in Saccharomyces cerevisiae.

21 January 2018

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Site-specific incorporation of an unnatural amino acid into proteins in mammalian cells.

21 January 2018

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Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells

21 January 2018

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Genetically encoding unnatural amino acids for cellular and neuronal studies

21 January 2018

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Site-specific incorporation of keto amino acids into functional G protein-coupled receptors using unnatural amino acid mutagenesis.

21 January 2018

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Expanding the genetic code of Escherichia coli

21 January 2018

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Site-specific incorporation of methyl- and acetyl-lysine analogues into recombinant proteins

21 January 2018

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Biosynthesis of a site-specific DNA cleaving protein

21 January 2018

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A genetically encoded bidentate, metal-binding amino acid

21 January 2018

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Genetic Incorporation of a Metal-Ion Chelating Amino Acid into Proteins as a Biophysical Probe

21 January 2018

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A facile system for encoding unnatural amino acids in mammalian cells

21 January 2018

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Breaking the degeneracy of the genetic code

21 January 2018

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Genetically encoding N(epsilon)-acetyllysine in recombinant proteins.

21 January 2018

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A promiscuous aminoacyl-tRNA synthetase that incorporates cysteine, methionine, and alanine homologs into proteins

21 January 2018

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Expanding the genetic repertoire of the methylotrophic yeast Pichia pastoris.

21 January 2018

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Adding l-lysine derivatives to the genetic code of mammalian cells with engineered pyrrolysyl-tRNA synthetases

21 January 2018

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In vivo incorporation of multiple unnatural amino acids through nonsense and frameshift suppression.

21 January 2018

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Probing the structure and function of the tachykinin neurokinin-2 receptor through biosynthetic incorporation of fluorescent amino acids at specific sites

21 January 2018

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Tyrosine residues that control binding and gating in the 5-hydroxytryptamine3 receptor revealed by unnatural amino acid mutagenesis.

21 January 2018

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Unnatural amino acids as probes of protein structure and function.

21 January 2018

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Improved amber and opal suppressor tRNAs for incorporation of unnatural amino acids in vivo. Part 1: minimizing misacylation

21 January 2018

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Improved amber and opal suppressor tRNAs for incorporation of unnatural amino acids in vivo. Part 2: evaluating suppression efficiency.

21 January 2018

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Introduction of specialty functions by the position-specific incorporation of nonnatural amino acids into proteins through four-base codon/anticodon pairs

21 January 2018

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An expanded genetic code with a functional quadruplet codon

21 January 2018

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Four-base codon mediated mRNA display to construct peptide libraries that contain multiple nonnatural amino acids

21 January 2018

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In vitro selection of tRNAs for efficient four-base decoding to incorporate non-natural amino acids into proteins in an Escherichia coli cell-free translation system

21 January 2018

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Five-base codons for incorporation of nonnatural amino acids into proteins

21 January 2018

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Four-base codon-mediated incorporation of non-natural amino acids into proteins in a eukaryotic cell-free translation system

21 January 2018

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Design of carrier tRNAs and selection of four-base codons for efficient incorporation of various nonnatural amino acids into proteins in Spodoptera frugiperda 21 (Sf21) insect cell-free translation system

21 January 2018

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Functional epitopes at the ribosome subunit interface.

21 January 2018

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Evolved orthogonal ribosomes enhance the efficiency of synthetic genetic code expansion.

21 January 2018

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Cellular logic with orthogonal ribosomes.

21 January 2018

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Synthesizing cellular networks from evolved ribosome-mRNA pairs.

21 January 2018

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Discovery of aminoacyl-tRNA synthetase activity through cell-surface display of noncanonical amino acids

21 January 2018

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High-throughput screening for methionyl-tRNA synthetases that enable residue-specific incorporation of noncanonical amino acids into recombinant proteins in bacterial cells

21 January 2018

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Reassignment of sense codons in vivo.

21 January 2018

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Nonnatural amino acid incorporation into the methionine 214 position of the metzincin Pseudomonas aeruginosa alkaline protease.

21 January 2018

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Elucidation of solvent exposure, side-chain reactivity, and steric demands of the trifluoromethionine residue in a recombinant protein.

21 January 2018

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Design of anti- and pro-aggregation variants to assess the effects of methionine oxidation in human prion protein

21 January 2018

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Cotranslational incorporation of a structurally diverse series of proline analogues in an Escherichia coli expression system.

21 January 2018

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Residue-specific bioincorporation of non-natural, biologically active amino acids into proteins as possible drug carriers: structure and stability of the per-thiaproline mutant of annexin V

21 January 2018

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Stabilization of bzip peptides through incorporation of fluorinated aliphatic residues.

21 January 2018

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Incorporation of trifluoroisoleucine into proteins in vivo

21 January 2018

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Stereoselective incorporation of an unsaturated isoleucine analogue into a protein expressed in E. coli.

21 January 2018

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Alternative translations of a single RNA message: an identity switch of (2S,3R)-4,4,4-trifluorovaline between valine and isoleucine codons

21 January 2018

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Cell surface labeling of Escherichia coli via copper(I)-catalyzed [3+2] cycloaddition

21 January 2018

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Processing of N-terminal unnatural amino acids in recombinant human interferon-beta in Escherichia coli

21 January 2018

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Aminotryptophan-containing barstar: structure--function tradeoff in protein design and engineering with an expanded genetic code.

21 January 2018

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Enzymes harboring unnatural amino acids: mechanistic and structural analysis of the enhanced catalytic activity of a glutathione transferase containing 5-fluorotryptophan

21 January 2018

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Expression of compstatin in Escherichia coli: incorporation of unnatural amino acids enhances its activity

21 January 2018

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Selective dye-labeling of newly synthesized proteins in bacterial cells.

21 January 2018

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Fluorinated Coiled-Coil Proteins Prepared In Vivo Display Enhanced Thermal and Chemical Stability This work was supported by a grant from the U.S. Army Research Office. Y. Tang is supported by a Whitaker Graduate Research Fellowship. We thank Dr. Ga

21 January 2018

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Selective identification of newly synthesized proteins in mammalian cells using bioorthogonal noncanonical amino acid tagging (BONCAT)

21 January 2018

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Labeling, detection and identification of newly synthesized proteomes with bioorthogonal non-canonical amino-acid tagging

21 January 2018

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Fluorescence visualization of newly synthesized proteins in mammalian cells

21 January 2018

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Photo-leucine and photo-methionine allow identification of protein-protein interactions in living cells

21 January 2018

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Identification of an expanded set of translationally active methionine analogues in Escherichia coli

21 January 2018

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Relaxing the substrate specificity of an aminoacyl-tRNA synthetase allows in vitro and in vivo synthesis of proteins containing unnatural amino acids

21 January 2018

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Substrate specificity is determined by amino acid binding pocket size in Escherichia coli phenylalanyl-tRNA synthetase

21 January 2018

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Amino acid substrate specificity of Escherichia coli phenylalanyl-tRNA synthetase altered by distinct mutations.

21 January 2018

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Biosynthesis of proteins incorporating a versatile set of phenylalanine analogues

21 January 2018

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Lithographic patterning of photoreactive cell-adhesive proteins

21 January 2018

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Biosynthesis and stability of coiled-coil peptides containing (2S,4R)-5,5,5-trifluoroleucine and (2S,4S)-5,5,5-trifluoroleucine

21 January 2018

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A designed phenylalanyl-tRNA synthetase variant allows efficient in vivo incorporation of aryl ketone functionality into proteins

21 January 2018

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Enlarging the amino acid set of Escherichia coli by infiltration of the valine coding pathway

21 January 2018

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Attenuation of the editing activity of the Escherichia coli leucyl-tRNA synthetase allows incorporation of novel amino acids into proteins in vivo

21 January 2018

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Selection and characterization of Escherichia coli variants capable of growth on an otherwise toxic tryptophan analogue

21 January 2018

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Evolution of a fluorinated green fluorescent protein

21 January 2018

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Synthesis and sequence optimization of GFP mutants containing aromatic non-natural amino acids at the Tyr66 position

21 January 2018

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Dissecting protein structure and function using directed evolution.

21 January 2018

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Directed evolution of novel protein functions.

21 January 2018

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How enzymes adapt: lessons from directed evolution

21 January 2018

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Protein evolution with an expanded genetic code

21 January 2018

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A helper phage to improve single-chain antibody presentation in phage display

21 January 2018

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Phage selection for site-specific incorporation of unnatural amino acids into proteins in vivo

21 January 2018

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Protein Engineering by In Vivo Incorporation of Non-Natural Amino Acids: Control of Incorporation of Methionine Analogues by Methionyl-tRNA Synthetase

21 January 2018

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Fluoroproline Flip-Flop: Regiochemical Reversal of a Stereoelectronic Effect on Peptide and Protein Structures

21 January 2018

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Evolving Proteins of Novel Composition

21 January 2018

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Influence of global fluorination on chloramphenicol acetyltransferase activity and stability

21 January 2018

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Fluorinated chloramphenicol acetyltransferase thermostability and activity profile: Improved thermostability by a single-isoleucine mutant

21 January 2018

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Translation: Duality in the genetic code

21 January 2018

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21 January 2018

Identifiers

DOI

10.1039/B909200P

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21 August 2017

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