(Q37832436)

24 August 2017

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title

Self-cleaving fusion tags for recombinant protein production (English)

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24 August 2017

Yifeng Li

1

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24 August 2017

26 January 2011

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24 August 2017

Biotechnology Letters

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24 August 2017

33

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24 August 2017

5

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24 August 2017

869-881

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https://scigraph.springernature.com/pub.10.1007/s10529-011-0533-8

24 August 2017

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Simple bioseparations using self-cleaving elastin-like polypeptide tags.

21 January 2018

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Novel and economical purification of recombinant proteins: intein-mediated protein purification using in vivo polyhydroxybutyrate (PHB) matrix association

21 January 2018

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Production of recombinant Conkunitzin-S1 in Escherichia coli.

21 January 2018

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Production of recombinant proteins in Escherichia coli using an N-terminal tag derived from sortase

21 January 2018

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Soluble expression and one-step purification of a neurotoxin Huwentoxin-I in Escherichia coli.

21 January 2018

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Expression of a cytotoxic cationic antibacterial peptide in Escherichia coli using two fusion partners

21 January 2018

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Expression and purification of antimicrobial peptide CM4 by Npro fusion technology in E. coli

21 January 2018

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Efficient system of artificial oil bodies for functional expression and purification of recombinant nattokinase in Escherichia coli

21 January 2018

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One-step purification of insoluble hydantoinase overproduced in Escherichia coli.

21 January 2018

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Protein splicing involving the Saccharomyces cerevisiae VMA intein. The steps in the splicing pathway, side reactions leading to protein cleavage, and establishment of an in vitro splicing system

21 January 2018

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Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element

21 January 2018

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Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step

21 January 2018

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Elimination of in vivo cleavage between target protein and intein in the intein-mediated protein purification systems.

21 January 2018

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Intein-mediated expression is an effective approach in the study of beta-defensins.

21 January 2018

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A novel, cheap and effective fusion expression system for the production of recombinant proteins.

21 January 2018

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Production and purification of recombinant human glucagon overexpressed as intein fusion protein in Escherichia coli.

21 January 2018

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Production of recombinant human epidermal growth factor using Ssp dnaB mini-intein system

21 January 2018

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Optimization of ELP-intein mediated protein purification by salt substitution.

21 January 2018

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21 January 2018

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Expression and characterization of human salivary statherin from Escherichia coli using two different fusion constructs

21 January 2018

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21 January 2018

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Yersinia pestis outer membrane type III secretion protein YscC: expression, purification, characterization, and induction of specific antiserum

21 January 2018

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Intein-mediated fusion expression, high efficient refolding, and one-step purification of gelonin toxin

21 January 2018

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Expression of N-terminal Cys-protein fragments using an intein refolding strategy.

21 January 2018

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Construction and use of low-copy number T7 expression vectors for purification of problem proteins: purification of mycobacterium tuberculosis RmlD and pseudomonas aeruginosa LasI and RhlI proteins, and functional analysis of purified RhlI

21 January 2018

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High yield expression and single step purification of human thionein/metallothionein

21 January 2018

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Simple protein purification through affinity adsorption on regenerated amorphous cellulose followed by intein self-cleavage

21 January 2018

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Expression of the class 1 outer-membrane protein of Neisseria meningitidis in Escherichia coli and purification using a self-cleavable affinity tag.

21 January 2018

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Structure of sortase, the transpeptidase that anchors proteins to the cell wall of Staphylococcus aureus

21 January 2018

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https://api.crossref.org/works/10.1007%2FS10529-011-0533-8

New and easy strategy for cloning, expression, purification, and characterization of the 5S subunit of transcarboxylase from Propionibacterium f. shermanii.

21 January 2018

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https://api.crossref.org/works/10.1007%2FS10529-011-0533-8

Cloning of a rumen fungal xylanase gene and purification of the recombinant enzyme via artificial oil bodies.

21 January 2018

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https://api.crossref.org/works/10.1007%2FS10529-011-0533-8

Expression and purification of glycine N-methyltransferases in Escherichia coli

21 January 2018

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https://api.crossref.org/works/10.1007%2FS10529-011-0533-8

Tagging for protein expression.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS10529-011-0533-8

Expression, purification, phosphorylation and characterization of recombinant human statherin.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS10529-011-0533-8

A self-cleavable sortase fusion for one-step purification of free recombinant proteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS10529-011-0533-8

Biotinylated-sortase self-cleavage purification (BISOP) method for cell-free produced proteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS10529-011-0533-8

Staphylococcus aureus sortase, an enzyme that anchors surface proteins to the cell wall

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS10529-011-0533-8

Protein purification via temperature-dependent, intein-mediated cleavage from an immobilized metal affinity resin

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS10529-011-0533-8

A novel "clip-and-link" activity of repeat in toxin (RTX) proteins from gram-negative pathogens. Covalent protein cross-linking by an Asp-Lys isopeptide bond upon calcium-dependent processing at an Asp-Pro bond

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS10529-011-0533-8

Expression and characterization of N-terminal domain of Epstein-Barr virus latent membrane protein 2A in Escherichia coli

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS10529-011-0533-8

Overexpression and purification of Helicobacter pylori flavodoxin and induction of a specific antiserum in rabbits.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS10529-011-0533-8

Protein splicing elements: inteins and exteins--a definition of terms and recommended nomenclature

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS10529-011-0533-8

Structure-function analysis of inositol hexakisphosphate-induced autoprocessing of the Vibrio cholerae multifunctional autoprocessing RTX toxin

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS10529-011-0533-8

Single-step affinity purification of recombinant proteins using a self-excising module from Neisseria meningitidis FrpC.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS10529-011-0533-8

MARTX, multifunctional autoprocessing repeats-in-toxin toxins

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS10529-011-0533-8

Matrix-assisted refolding of autoprotease fusion proteins on an ion exchange column: a kinetic investigation.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS10529-011-0533-8

Autoprocessing of the Vibrio cholerae RTX toxin by the cysteine protease domain

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS10529-011-0533-8

Mechanistic and structural insights into the proteolytic activation of Vibrio cholerae MARTX toxin

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS10529-011-0533-8

Simplified, enhanced protein purification using an inducible, autoprocessing enzyme tag

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS10529-011-0533-8

Expression and purification of moricin CM4 and human β-defensins 4 in Escherichia coli using a new technology.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS10529-011-0533-8

Single step intein-mediated purification of hGMCSF expressed in salt-inducible E. coli

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS10529-011-0533-8

Use of Ssp dnaB derived mini-intein as a fusion partner for production of recombinant human brain natriuretic peptide in Escherichia coli.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS10529-011-0533-8

Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS10529-011-0533-8

Cloning and nucleotide sequence of frpC, a second gene from Neisseria meningitidis encoding a protein similar to RTX cytotoxins

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS10529-011-0533-8

A method for expression and purification of soluble, active Hsp47, a collagen-specific molecular chaperone

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS10529-011-0533-8

Anchoring of surface proteins to the cell wall of Staphylococcus aureus. Cysteine 184 and histidine 120 of sortase form a thiolate-imidazolium ion pair for catalysis

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS10529-011-0533-8

Tagging recombinant proteins to enhance solubility and aid purification.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS10529-011-0533-8

Cloning, nucleotide sequence and expression of a hydantoinase and carbamoylase gene from Arthrobacter aurescens DSM 3745 in Escherichia coli and comparison with the corresponding genes from Arthrobacter aurescens DSM 3747.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS10529-011-0533-8

LTCI, a novel chymotrypsin inhibitor of the potato I family from the earthworm Lumbricus terrestris. Purification, cDNA cloning, and expression

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS10529-011-0533-8

High-level expression and rapid purification of rare-codon genes from hyperthermophilic archaea by the GST gene fusion system

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS10529-011-0533-8

Single-column purification and bio-characterization of recombinant human parathyroid hormone-related protein (1-139).

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS10529-011-0533-8

Recombinant protein purification by self-cleaving aggregation tag.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS10529-011-0533-8

Effect of location of the His-tag on the production of soluble and functional Buthus martensii Karsch insect toxin

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS10529-011-0533-8

Intein-mediated rapid purification of recombinant human pituitary adenylate cyclase activating polypeptide

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS10529-011-0533-8

Intein-mediated rapid purification and characterization of a novel recombinant agonist for VPAC2.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS10529-011-0533-8

A novel recombinant, VPAC2-selective agonist enhancing insulin release and glucose disposal.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS10529-011-0533-8

Intein-mediated rapid purification of recombinant maxadilan and M65 and their acute effects on plasma glucose

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS10529-011-0533-8

Microbial polyhydroxyalkanote synthesis repression protein PhaR as an affinity tag for recombinant protein purification

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS10529-011-0533-8

10.1007/S10529-011-0533-8

21 January 2018

Identifiers

DOI

1 reference

24 August 2017

1 reference

PubMed ID

21267760