(Q38354263)

2 September 2017

title

Expression, purification, and isotope labeling of the Fv of the human HIV-1 neutralizing antibody 447-52D for NMR studies (English)

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2 September 2017

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Michal Sharon

4

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Naama Kessler

1

object named as

Naama Kessler

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2 September 2017

9

Jacob Anglister

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2 September 2017

Anat Zvi

2

1 reference

2 September 2017

Min Ji

3

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2 September 2017

Osnat Rosen

5

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2 September 2017

Rina Levy

6

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2 September 2017

Miroslaw Gorny

7

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2 September 2017

Suzan Zolla-Pazner

8

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2 September 2017

publication date

1 June 2003

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Protein Expression and Purification

2 September 2017

published in

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2 September 2017

volume

29

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2 September 2017

issue

2

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2 September 2017

page(s)

291-303

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Continuous cultures of fused cells secreting antibody of predefined specificity

2 September 2017

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A comparison of strategies to stabilize immunoglobulin Fv-fragments

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Redox state of single chain Fv fragments targeted to the endoplasmic reticulum, cytosol and mitochondria

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Characterization of the Erwinia carotovora pelB gene and its product pectate lyase

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Export of hybrid proteins FhuA'-'LacZ and FhuA'-'PhoA to the cell envelope of Escherichia coli K-12.

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The Effect of Folding Catalysts on the In Vivo Folding Process of Different Antibody Fragments Expressed in Escherichia coli

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"Diabodies": small bivalent and bispecific antibody fragments

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Tumor localization of anti-CEA single-chain Fvs: improved targeting by non-covalent dimers

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High level Escherichia coli expression and production of a bivalent humanized antibody fragment

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Antibodies that inhibit fusion of human immunodeficiency virus-infected cells bind a 24-amino acid sequence of the viral envelope, gp120

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Type-specific neutralization of the human immunodeficiency virus with antibodies to env-encoded synthetic peptides

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Human immunodeficiency virus type 1 neutralization epitope with conserved architecture elicits early type-specific antibodies in experimentally infected chimpanzees

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Mapping of epitopes exposed on intact human immunodeficiency virus type 1 (HIV-1) virions: a new strategy for studying the immunologic relatedness of HIV-1.

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Human monoclonal antibodies specific for conformation-sensitive epitopes of V3 neutralize human immunodeficiency virus type 1 primary isolates from various clades.

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Neutralization of primary human immunodeficiency virus type 1 isolates by the broadly reactive anti-V3 monoclonal antibody, 447-52D

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Neutralization of HIV-1 primary isolates by polyclonal and monoclonal human antibodies

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Additive Effects Characterize the Interaction of Antibodies Involved in Neutralization of the Primary Dualtropic Human Immunodeficiency Virus Type 1 Isolate 89.6

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7 January 2021

Expression, purification, and isotope labeling of a gp120 V3 peptide and production of a Fab from a HIV-1 neutralizing antibody for NMR studies.

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Calculation of protein extinction coefficients from amino acid sequence data

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Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

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Attenuated T2 relaxation by mutual cancellation of dipole-dipole coupling and chemical shift anisotropy indicates an avenue to NMR structures of very large biological macromolecules in solution

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Affinity maturation of a high-affinity human monoclonal antibody against the third hypervariable loop of human immunodeficiency virus: use of phage display to improve affinity and broaden strain reactivity

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Use of a novel mutagenesis strategy, optimized residue substitution, to decrease the off-rate of an anti-gp120 antibody

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7 January 2021

Contribution of arginine residues in the RP135 peptide derived from the V3 loop of gp120 to its interaction with the Fv fragment of the 0.5beta HIV-1 neutralizing antibody

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7 January 2021

Site-directed mutagenesis by overlap extension using the polymerase chain reaction

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7 January 2021

Structure of an HIV gp120 envelope glycoprotein in complex with the CD4 receptor and a neutralizing human antibody

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7 January 2021

Uniform labeling of a recombinant antibody Fv-fragment with 15N and 13C for heteronuclear NMR spectroscopy.

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7 January 2021

Production and characterization of an antibody Fv fragment 15N-labeled in the VL domain only

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7 January 2021

Structural and Dynamic Properties of the Fv Fragment and the Single-Chain Fv Fragment of an Antibody in Solution Investigated by Heteronuclear 3D NMR Spectroscopy

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7 January 2021

Backbone resonance assignments for the Fv fragment of the catalytic antibody NPN43C9 with bound p-nitrophenol

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Reduction in the amide hydrogen exchange rates of an anti-lysozyme Fv fragment due to formation of the Fv-lysozyme complex

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7 January 2021

Backbone and C beta assignments of the anti-gp120 antibody Fv fragment complexed with an antigenic peptide

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7 January 2021

A cis proline turn linking two beta-hairpin strands in the solution structure of an antibody-bound HIV-1IIIB V3 peptide

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7 January 2021

NMR structure of an anti-gp120 antibody complex with a V3 peptide reveals a surface important for co-receptor binding

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7 January 2021

A model of a gp120 V3 peptide in complex with an HIV-neutralizing antibody based on NMR and mutant cycle-derived constraints

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7 January 2021

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7 January 2021

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7 January 2021

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Identifiers

DOI

1 reference

2 September 2017

1 reference