(Q38981249)

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:27742731%20AND%20SRC:MED&resulttype=core&format=json

25 March 2020

review article

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title

Cell-Free Synthetic Biology: Engineering Beyond the Cell (English)

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:27742731%20AND%20SRC:MED&resulttype=core&format=json

25 March 2020

1 reference

2

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:27742731%20AND%20SRC:MED&resulttype=core&format=json

25 March 2020

author name string

Jessica G Perez

series ordinal

1

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:27742731%20AND%20SRC:MED&resulttype=core&format=json

25 March 2020

Michael C Jewett

series ordinal

3

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:27742731%20AND%20SRC:MED&resulttype=core&format=json

25 March 2020

publication date

1 December 2016

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:27742731%20AND%20SRC:MED&resulttype=core&format=json

Cold Spring Harbor Perspectives in Biology

25 March 2020

published in

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:27742731%20AND%20SRC:MED&resulttype=core&format=json

25 March 2020

volume

8

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:27742731%20AND%20SRC:MED&resulttype=core&format=json

25 March 2020

issue

12

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:27742731%20AND%20SRC:MED&resulttype=core&format=json

Production of secretory and extracellular N-linked glycoproteins in Escherichia coli.

25 March 2020

cites work

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12 December 2020

Optimization of a miniaturized fluid array device for cell-free protein synthesis.

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12 December 2020

Global RNA half-life analysis in Escherichia coli reveals positional patterns of transcript degradation

1 reference

12 December 2020

Simultaneous expression and maturation of the iron-sulfur protein ferredoxin in a cell-free system

1 reference

12 December 2020

Flexizyme-mediated genetic reprogramming as a tool for noncanonical peptide synthesis and drug discovery

1 reference

12 December 2020

Linear DNA for rapid prototyping of synthetic biological circuits in an Escherichia coli based TX-TL cell-free system

1 reference

12 December 2020

RF1 knockout allows ribosomal incorporation of unnatural amino acids at multiple sites

1 reference

12 December 2020

Species-independent translational leaders facilitate cell-free expression

1 reference

12 December 2020

Synthesis of 2.3 mg/ml of protein with an all Escherichia coli cell-free transcription-translation system.

1 reference

12 December 2020

Using E. coli-based cell-free protein synthesis to evaluate the kinetic performance of an orthogonal tRNA and aminoacyl-tRNA synthetase pair

1 reference

12 December 2020

In vitro Fab display: a cell-free system for IgG discovery

1 reference

12 December 2020

Validation of an entirely in vitro approach for rapid prototyping of DNA regulatory elements for synthetic biology

1 reference

12 December 2020

Laccases and their applications: a patent review

1 reference

12 December 2020

Expanding the genetic code of Escherichia coli with phosphoserine

1 reference

12 December 2020

Cell-free protein synthesis enables high yielding synthesis of an active multicopper oxidase.

1 reference

12 December 2020

Leishmania cell-free protein expression system.

1 reference

12 December 2020

Production of bispecific antibodies in "knobs-into-holes" using a cell-free expression system

1 reference

12 December 2020

Genome replication, synthesis, and assembly of the bacteriophage T7 in a single cell-free reaction.

1 reference

12 December 2020

The past, present and future of cell-free protein synthesis

1 reference

12 December 2020

Engineering toward a bacterial "endoplasmic reticulum" for the rapid expression of immunoglobulin proteins

1 reference

12 December 2020

Directed Evolution of Proteins through In Vitro Protein Synthesis in Liposomes

1 reference

12 December 2020

Tobacco BY-2 cell-free lysate: an alternative and highly-productive plant-based in vitro translation system

1 reference

12 December 2020

Energizing eukaryotic cell-free protein synthesis with glucose metabolism.

1 reference

12 December 2020

Cell-free expression systems for eukaryotic protein production

1 reference

12 December 2020

Coping with complexity: machine learning optimization of cell-free protein synthesis.

1 reference

12 December 2020

An engineered Escherichia coli tyrosyl-tRNA synthetase for site-specific incorporation of an unnatural amino acid into proteins in eukaryotic translation and its application in a wheat germ cell-free system

1 reference

12 December 2020

Rapid cell-free forward engineering of novel genetic ring oscillators

1 reference

12 December 2020

Integration of biological parts toward the synthesis of a minimal cell

1 reference

12 December 2020

Construction of an in vitro bistable circuit from synthetic transcriptional switches

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12 December 2020

GlycoDelete engineering of mammalian cells simplifies N-glycosylation of recombinant proteins.

1 reference

12 December 2020

An in vitro microfluidic approach to generating protein-interaction networks

1 reference

12 December 2020

Expanding the genetic code

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12 December 2020

Cooperative working of bacterial chromosome replication proteins generated by a reconstituted protein expression system

1 reference

12 December 2020

A tightly regulated inducible expression system utilizing the fim inversion recombination switch

1 reference

12 December 2020

A highly flexible tRNA acylation method for non-natural polypeptide synthesis.

1 reference

12 December 2020

Regulated in vitro synthesis of Escherichia coli tryptophan operon messenger ribonucleic acid and enzymes

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12 December 2020

Gene position more strongly influences cell-free protein expression from operons than T7 transcriptional promoter strength

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12 December 2020

Cell-free protein synthesis in microfluidic 96-well plates

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12 December 2020

Overview of cell-free protein synthesis: historic landmarks, commercial systems, and expanding applications

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12 December 2020

High-throughput preparation methods of crude extract for robust cell-free protein synthesis

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12 December 2020

Genetically encoding N(epsilon)-methyl-L-lysine in recombinant histones.

1 reference

12 December 2020

Thrombin-mediated transcriptional regulation using DNA aptamers in DNA-based cell-free protein synthesis

1 reference

12 December 2020

Flexizymes for genetic code reprogramming

1 reference

12 December 2020

Measuring riboswitch activity in vitro and in artificial cells with purified transcription-translation machinery

1 reference

12 December 2020

High-throughput synthesis of stable isotope-labeled transmembrane proteins for targeted transmembrane proteomics using a wheat germ cell-free protein synthesis system

1 reference

12 December 2020

Functional analysis of membranous Fo-a subunit of F1Fo-ATP synthase by in vitro protein synthesis.

1 reference

12 December 2020

Cell-Free Protein Synthesis: Pros and Cons of Prokaryotic and Eukaryotic Systems

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12 December 2020

Energizing cell-free protein synthesis with glucose metabolism

1 reference

12 December 2020

Characterizing IGR IRES-mediated translation initiation for use in yeast cell-free protein synthesis

1 reference

12 December 2020

An E. coli cell-free expression toolbox: application to synthetic gene circuits and artificial cells.

1 reference

12 December 2020

Site-specific incorporation of a redox-active amino acid into proteins

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12 December 2020

Sealable femtoliter chamber arrays for cell-free biology

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12 December 2020

Cell-free expression--making a mark.

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12 December 2020

Semi-synthetic minimal cells: origin and recent developments

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12 December 2020

Cell-free protein synthesis systems derived from cultured mammalian cells

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12 December 2020

Construction of a genetic toggle switch in Escherichia coli

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12 December 2020

Cell-free translation reconstituted with purified components

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12 December 2020

Cap-dependent and cap-independent translation by internal initiation of mRNAs in cell extracts prepared from Saccharomyces cerevisiae

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12 December 2020

An economical method for cell-free protein synthesis using glucose and nucleoside monophosphates

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12 December 2020

High-level cell-free synthesis yields of proteins containing site-specific non-natural amino acids

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12 December 2020

Programming peptidomimetic syntheses by translating genetic codes designed de novo

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12 December 2020

In vitro genetic reconstruction of bacterial transcription initiation by coupled synthesis and detection of RNA polymerase holoenzyme

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12 December 2020

Efficient decoding of the UAG triplet as a full-fledged sense codon enhances the growth of a prfA-deficient strain of Escherichia coli

1 reference

12 December 2020

Engineering Transcriptional Regulator Effector Specificity Using Computational Design and In Vitro Rapid Prototyping: Developing a Vanillin Sensor.

1 reference

12 December 2020

Efficient cell-free production of olfactory receptors: detergent optimization, structure, and ligand binding analyses

1 reference

12 December 2020

Preparation of N-acylated proteins modified with fatty acids having a specific chain length using an insect cell-free protein synthesis system

1 reference

12 December 2020

Stochasticity in gene expression in a cell-sized compartment

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12 December 2020

Wheat germ cell-free expression: Two detergents with a low critical micelle concentration allow for production of soluble HCV membrane proteins

1 reference

12 December 2020

Assessing sequence plasticity of a virus-like nanoparticle by evolution toward a versatile scaffold for vaccines and drug delivery

1 reference

12 December 2020

Cellular logic with orthogonal ribosomes.

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12 December 2020

Detection and characterization of a multicopper oxidase from Nitrosomonas europaea.

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12 December 2020

A synthetic oscillatory network of transcriptional regulators

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12 December 2020

Production of multi-subunit complexes on liposome through an E. coli cell-free expression system

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12 December 2020

Assembly and operation of bacterial tripartite multidrug efflux pumps.

1 reference

12 December 2020

Evolution of translation initiation sequences using in vitro yeast ribosome display.

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12 December 2020

Upgrading protein synthesis for synthetic biology.

1 reference

12 December 2020

Time-resolved analysis of biological reactions based on heterogeneous assays in liquid plugs of nanoliter volume

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12 December 2020

Cell-free protein synthesis system prepared from insect cells by freeze-thawing

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12 December 2020

Assembly of β-barrel proteins into bacterial outer membranes

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12 December 2020

A cell-free translocation system using extracts of cultured insect cells to yield functional membrane proteins.

1 reference

12 December 2020

Improvement of isopropanol production by metabolically engineered Escherichia coli using gas stripping

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12 December 2020

Site-specific incorporation of p-propargyloxyphenylalanine in a cell-free environment for direct protein-protein click conjugation

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12 December 2020

Characterizing and alleviating substrate limitations for improved in vitro ribosome construction.

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12 December 2020

Synthetic gene networks that count

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12 December 2020

High-yield cell-free protein synthesis for site-specific incorporation of unnatural amino acids at two sites.

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12 December 2020

Production of the antimalarial drug precursor artemisinic acid in engineered yeast

1 reference

12 December 2020

A constructed microbial consortium for biodegradation of the organophosphorus insecticide parathion

1 reference

12 December 2020

Peptide surfactants for cell-free production of functional G protein-coupled receptors

1 reference

12 December 2020

Comprehensive study of liposome-assisted synthesis of membrane proteins using a reconstituted cell-free translation system.

1 reference

12 December 2020

Real-time mRNA measurement during an in vitro transcription and translation reaction using binary probes.

1 reference

12 December 2020

Environmental signal integration by a modular AND gate

1 reference

12 December 2020

Robust production of recombinant phosphoproteins using cell-free protein synthesis

1 reference

12 December 2020

An efficient mammalian cell-free translation system supplemented with translation factors

1 reference

12 December 2020

Non-standard amino acid incorporation into proteins using Escherichia coli cell-free protein synthesis

1 reference

12 December 2020

A versatile coupled cell-free transcription-translation system based on tobacco BY-2 cell lysates.

1 reference

12 December 2020

Site-specific labeling of proteins for single-molecule FRET measurements using genetically encoded ketone functionalities

1 reference

12 December 2020

The PURE system for protein production.

1 reference

12 December 2020

Coarse-grained dynamics of protein synthesis in a cell-free system.

1 reference

12 December 2020

Addition of p-azido-L-phenylalanine to the genetic code of Escherichia coli

1 reference

12 December 2020

Expression optimization and synthetic gene networks in cell-free systems.

1 reference

12 December 2020

Cell-free expression and assembly of ATP synthase.

1 reference

12 December 2020

Iterative plug-and-play methodology for constructing and modifying synthetic gene networks

1 reference

12 December 2020

Multiple-site labeling of proteins with unnatural amino acids.

1 reference

12 December 2020

Mitochondrial ATP synthase disorders: molecular mechanisms and the quest for curative therapeutic approaches

1 reference

12 December 2020

Efficient generation of insect-based cell-free translation extracts active in glycosylation and signal sequence processing

1 reference

12 December 2020

Protein synthesis by pure translation systems.

1 reference

12 December 2020

A vaccine directed to B cells and produced by cell-free protein synthesis generates potent antilymphoma immunity

1 reference

12 December 2020

Production of site-specific antibody-drug conjugates using optimized non-natural amino acids in a cell-free expression system

1 reference

12 December 2020

Streamlined extract preparation for Escherichia coli-based cell-free protein synthesis by sonication or bead vortex mixing.

1 reference

12 December 2020

A simplified and robust protocol for immunoglobulin expression in Escherichia coli cell-free protein synthesis systems

1 reference

12 December 2020

Cell-free protein synthesis from a release factor 1 deficient Escherichia coli activates efficient and multiple site-specific nonstandard amino acid incorporation

1 reference

12 December 2020

Traceless and site-specific ubiquitination of recombinant proteins

1 reference

12 December 2020

Cell-free protein synthesis and assembly on a biochip.

1 reference

12 December 2020

Production and stabilization of the trimeric influenza hemagglutinin stem domain for potentially broadly protective influenza vaccines

1 reference

12 December 2020

The dependence of cell-free protein synthesis in E. coli upon naturally occurring or synthetic polyribonucleotides

1 reference

12 December 2020

Programmable ligand-controlled riboregulators of eukaryotic gene expression.

1 reference

12 December 2020

Advances in cell-free protein synthesis for the functional and structural analysis of membrane proteins

1 reference

12 December 2020

Genetically expanded cell-free protein synthesis using endogenous pyrrolysyl orthogonal translation system

1 reference

12 December 2020

Study of messenger RNA inactivation and protein degradation in an Escherichia coli cell-free expression system.

1 reference

12 December 2020

Cell-free protein synthesis inside giant unilamellar vesicles analyzed by flow cytometry.

1 reference

12 December 2020

Developing cell-free biology for industrial applications

1 reference

12 December 2020

Multi-input regulation and logic with T7 promoters in cells and cell-free systems

1 reference

12 December 2020

Compartmentalization of an all-E. coli Cell-Free Expression System for the Construction of a Minimal Cell.

1 reference

12 December 2020

Aglycosylated antibodies and antibody fragments produced in a scalable in vitro transcription-translation system

1 reference

12 December 2020

Synthesis of milligram quantities of proteins using a reconstituted in vitro protein synthesis system.

1 reference

12 December 2020

Efficient synthesis of a disulfide‐containing protein through a batch cell‐free system from wheat germ

1 reference

12 December 2020

One-pot system for synthesis, assembly, and display of functional single-span membrane proteins on oil-water interfaces

1 reference

12 December 2020

Genomically recoded organisms expand biological functions

1 reference

12 December 2020

Wheat germ systems for cell-free protein expression

1 reference

12 December 2020

A cell-free protein synthesis system for high-throughput proteomics

1 reference

12 December 2020

Optimized extract preparation methods and reaction conditions for improved yeast cell-free protein synthesis

1 reference

12 December 2020

Design and construction of a double inversion recombination switch for heritable sequential genetic memory

1 reference

12 December 2020

A method for genetically installing site-specific acetylation in recombinant histones defines the effects of H3 K56 acetylation

1 reference

12 December 2020

Principles of cell-free genetic circuit assembly

1 reference

12 December 2020

Cell-free systems: functional modules for synthetic and chemical biology

1 reference

12 December 2020

Production of functional antibody fragments in a vesicle-based eukaryotic cell-free translation system.

1 reference

12 December 2020

Cell-free protein synthesis: applications come of age.

1 reference

12 December 2020

A cell-free expression and purification process for rapid production of protein biologics.

1 reference

12 December 2020

Gene circuit performance characterization and resource usage in a cell-free "breadboard".

1 reference

12 December 2020

Cell-free synthesis of the H-cluster: a model for the in vitro assembly of metalloprotein metal centers.

1 reference

12 December 2020

Membrane protein synthesis in cell-free systems: from bio-mimetic systems to bio-membranes

1 reference

12 December 2020

Wheat germ cell-free system-based production of malaria proteins for discovery of novel vaccine candidates

1 reference

12 December 2020

Towards the construction of expressed proteomes using a Leishmania tarentolae based cell-free expression system

1 reference

12 December 2020

Modifications of wheat germ cell-free system for functional proteomics of plant membrane proteins

1 reference

12 December 2020

Refactoring bacteriophage T7

1 reference

12 December 2020

Toward Microfluidic Reactors for Cell-Free Protein Synthesis at the Point-of-Care

1 reference

12 December 2020

Enhanced phosphoserine insertion during Escherichia coli protein synthesis via partial UAG codon reassignment and release factor 1 deletion

1 reference

12 December 2020

Environmental genome shotgun sequencing of the Sargasso Sea

1 reference

12 December 2020

Principles of genetic circuit design

1 reference

12 December 2020

Improving cell-free protein synthesis through genome engineering of Escherichia coli lacking release factor 1

1 reference

12 December 2020

An integrated cell-free metabolic platform for protein production and synthetic biology

1 reference

12 December 2020

Pharmacological significance of glycosylation in therapeutic proteins

1 reference

12 December 2020

Creating small transcription activating RNAs

1 reference

12 December 2020

Rapidly characterizing the fast dynamics of RNA genetic circuitry with cell-free transcription-translation (TX-TL) systems

1 reference

12 December 2020

Cell-free synthesis of membrane proteins: tailored cell models out of microsomes.

1 reference

12 December 2020

Randomized BioBrick assembly: a novel DNA assembly method for randomizing and optimizing genetic circuits and metabolic pathways

1 reference

12 December 2020

In vitro integration of ribosomal RNA synthesis, ribosome assembly, and translation

1 reference

12 December 2020

Cell-free protein synthesis of a cytotoxic cancer therapeutic: Onconase production and a just-add-water cell-free system

1 reference

12 December 2020

An optimized yeast cell-free system: sufficient for translation of human papillomavirus 58 L1 mRNA and assembly of virus-like particles

1 reference

12 December 2020

The cost-efficiency realization in the Escherichia coli-based cell-free protein synthesis systems

1 reference

12 December 2020

Paper-based synthetic gene networks

1 reference

12 December 2020

Site-specific incorporation of biophysical probes into proteins

1 reference

12 December 2020

Humanization of yeast to produce complex terminally sialylated glycoproteins

1 reference

12 December 2020

Higher-order cellular information processing with synthetic RNA devices

1 reference

12 December 2020

Cell-Free Synthesis of a Functional Membrane Transporter into a Tethered Bilayer Lipid Membrane.

1 reference

12 December 2020

The stimulatory effect of cyclic adenosine 3'5'-monophosphate on DNA-directed synthesis of beta-galactosidase in a cell-free system

1 reference

12 December 2020

Protein synthesis yield increased 72 times in the cell-free PURE system.

1 reference

12 December 2020

The impact of transcriptional tuning on in vitro integrated rRNA transcription and ribosome construction

1 reference

12 December 2020

A combined cell-free transcription-translation system from Saccharomyces cerevisiae for rapid and robust protein synthe

1 reference

12 December 2020

Human protein factory for converting the transcriptome into an in vitro-expressed proteome,.

1 reference

12 December 2020

Construction of a protein library of Arabidopsis transcription factors using a wheat cell-free protein production system and its application for DNA binding analysis.

1 reference

12 December 2020

Cell-free synthesis and characterization of a novel cytotoxic pierisin-like protein from the cabbage butterfly Pieris rapae

1 reference

12 December 2020

Production of eukaryotic cell-free lysate from Leishmania tarentolae

1 reference

12 December 2020

Microscale to manufacturing scale-up of cell-free cytokine production--a new approach for shortening protein production development timelines

1 reference

12 December 2020

In vitro synthesis of the E. coli Sec translocon from DNA

1 reference

12 December 2020

An engineered eukaryotic protein glycosylation pathway in Escherichia coli.

1 reference

12 December 2020

Real-time monitoring of cell-free protein synthesis on a surface plasmon resonance chip.

1 reference

12 December 2020

Cell-free co-production of an orthogonal transfer RNA activates efficient site-specific non-natural amino acid incorporation.

1 reference

12 December 2020

The All E. coli TX-TL Toolbox 2.0: A Platform for Cell-Free Synthetic Biology.

1 reference

12 December 2020

Substrate replenishment and byproduct removal improve yeast cell-free protein synthesis

1 reference

12 December 2020

A highly efficient and robust cell-free protein synthesis system prepared from wheat embryos: plants apparently contain a suicide system directed at ribosomes

1 reference

12 December 2020

A prokaryote-based cell-free translation system that efficiently synthesizes glycoproteins.

1 reference

12 December 2020

Selection of fluorescent aptamer beacons that light up in the presence of zinc

1 reference

12 December 2020

Purified cell-free systems as standard parts for synthetic biology

1 reference

12 December 2020

Escherichia coli-based cell-free synthesis of virus-like particles.

1 reference

12 December 2020

A continuous-exchange cell-free protein synthesis system based on extracts from cultured insect cells

1 reference

12 December 2020

10.1101/CSHPERSPECT.A023853

Identifiers

DOI

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:27742731%20AND%20SRC:MED&resulttype=core&format=json

25 March 2020

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:27742731%20AND%20SRC:MED&resulttype=core&format=json

25 March 2020

1 reference