(Q39125159)

English

Preparation of fully active ficin from Ficus glabrata by covalent chromatography and characterization of its active centre by using 2,2'-depyridyl disulphide as a reactivity probe

scientific article published on November 1, 1976

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scholarly article

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Europe PubMed Central

PMC publication ID

9 September 2017

Preparation of fully active ficin from Ficus glabrata by covalent chromatography and characterization of its active centre by using 2,2'-depyridyl disulphide as a reactivity probe (English)

1 reference

Europe PubMed Central

PubMed publication ID

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A11777

7 October 2022

object named as

Ficus glabrata

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based on heuristic

inferred from title

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Europe PubMed Central

PubMed publication ID

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A11777

7 October 2022

1 reference

Europe PubMed Central

PubMed publication ID

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A11777

7 October 2022

2,2′-bipyridine

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Europe PubMed Central

PubMed publication ID

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A11777

7 October 2022

1

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J. P. Malthouse

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A11777

7 October 2022

author name string

K. Brocklehurst

2

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A11777

7 October 2022

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publication date

1 November 1976

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9 September 2017

full work available at URL

https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/11777/pdf/?tool=EBI

Portable Document Format

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Europe PubMed Central

PubMed publication ID

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A11777

7 October 2022

https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/11777/?tool=EBI

online access status

1 reference

Europe PubMed Central

PubMed publication ID

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A11777

7 October 2022

https://europepmc.org/articles/PMC1164109

online access status

content deliverer

Europe PubMed Central

1 reference

Europe PubMed Central

PubMed publication ID

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A11777

7 October 2022

https://europepmc.org/articles/PMC1164109?pdf=render

Portable Document Format

online access status

content deliverer

Europe PubMed Central

1 reference

Europe PubMed Central

PubMed publication ID

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A11777

7 October 2022

Biochemical Journal

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PMC publication ID

9 September 2017

159

1 reference

Europe PubMed Central

PMC publication ID

9 September 2017

2

1 reference

Europe PubMed Central

PMC publication ID

9 September 2017

221-234

1 reference

Europe PubMed Central

PMC publication ID

9 September 2017

Specificity of induced conformational changes. Case of yeast glyceraldehyde-3-phosphate dehydrogenase

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1164109

9 September 2017

4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole as a reactivity probe for the investigation of the thiol proteinases. evidence that ficin and bromelain may lack carboxyl groups conformationally equivalent to that of aspartic acid-158 of papain

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1164109

9 September 2017

PH-dependence of the steady-state rate of a two-step enzymic reaction

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1164109

9 September 2017

A reporter group delivery system with both absolute and selective specificity for thiol groups and an improved fluorescent probe containing the 7-nitrobenzo-2-oxa-1,3-diazole moiety

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1164109

9 September 2017

The case for assigning a value of approximately 4 to pKaI of the essential histidine-cysteine interactive systems of papain, bromelain and ficin

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1164109

14 September 2017

The mechanism of ficin-catalysed reactions

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1164109

15 June 2018

Ficincatalysed reactions: the affinity of ficin for some arginine derivatives

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1164109

15 June 2018

The location of the active-site histidine residue in the primary sequence of papain

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1164109

15 June 2018

The amino acid sequence around the active-site cysteine and histidine residues, and the buried cysteine residue in ficin

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1164109

15 June 2018

Reactivities of the various protonic states in the reactions of papain and of L-cysteine with 2,2'- and with 4,4'- dipyridyl disulphide: evidence for nucleophilic reactivity in the un-ionized thiol group of the cysteine-25 residue of papain occasion

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1164109

15 June 2018

The activation reaction of papain.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1164109

15 June 2018

The Hydrolysis of p-Nitrophenylacetate in Water. Mechanism and Method of Measurement

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1164109

15 June 2018

Covalent chromatography. Preparation of fully active papain from dried papaya latex

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1164109

15 June 2018

Reactions of papain and of low-molecular-weight thiols with some aromatic disulphides. 2,2'-Dipyridyl disulphide as a convenient active-site titrant for papain even in the presence of other thiols

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1164109

15 June 2018

Propapain and its Conversion to Papain: A New Type of Zymogen Activation Mechanism involving Intramolecular Thiol-Disulphide Interchange

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1164109

15 June 2018

On the specificity and pH dependence of ficin-catalyzed hydrolyses. Some comparisons with bromelain specificity.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1164109

15 June 2018

The unreliability of estimates of group dissociation constants

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1164109

15 June 2018

Ficin-Catalyzed Reactions. Hydrolysis of alpha-N-Benzoyl-l-Arginine Ethyl Ester and alpha-N-Benzoyl-l-Argininamide

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1164109

21 August 2018

DIRECT EVIDENCE FOR AN ACYLATED THIOL AS AN INTERMEDIATE IN PAPAIN- AND FICIN-CATALYSED HYDROLYSES

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1164109

21 August 2018

A novel reactivity of papain and a convenient active site titration in the presence of other thiols

1 reference

https://pubmed.ncbi.nlm.nih.gov/11777

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Papain- and ficin-catalyzed reactions. Effect of pH on activity and conformation of ficin

1 reference

https://pubmed.ncbi.nlm.nih.gov/11777

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Ficins (E.C. 3.4.22.3). Purification and characterization of the enzymic components of the latex of Ficus glabrata

1 reference

https://pubmed.ncbi.nlm.nih.gov/11777

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Covalent chromatography by thiol-disulfide interchange

1 reference

https://pubmed.ncbi.nlm.nih.gov/11777

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

THE CHEMICAL REACTIVITY OF THE THIOL GROUP IN THE ACTIVE CENTRE OF FICIN

1 reference

https://pubmed.ncbi.nlm.nih.gov/11777

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Crystalline papain. I. Preparation, specificity, and activation

1 reference

https://pubmed.ncbi.nlm.nih.gov/11777

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Purification of ficin by affinity chromatography

1 reference

https://pubmed.ncbi.nlm.nih.gov/11777

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Studies on ficin. I. Its isolation and characterization

1 reference

https://pubmed.ncbi.nlm.nih.gov/11777

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Determination of sulfhydryl groups with 2,2′- or 4,4′-dithiodipyridine

1 reference

https://pubmed.ncbi.nlm.nih.gov/11777

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

A study of the number and reactivity of the sulfhydryl groups of ficin

1 reference

https://pubmed.ncbi.nlm.nih.gov/11777

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

The preparation and properties of two new chromogenic substrates of trypsin

1 reference

https://pubmed.ncbi.nlm.nih.gov/11777

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

The pH‐Dependence of Rates of Individual Steps in Ficin Catalysis

1 reference

https://pubmed.ncbi.nlm.nih.gov/11777

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

FICUS ENZYMES. I. SEPARATION OF THE PROTEOLYTIC ENZYMES OF FICUS CARICA AND FICUS GLABRATA LATICES

1 reference

https://pubmed.ncbi.nlm.nih.gov/11777

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Identifiers

10.1042/BJ1590221

1 reference

Europe PubMed Central

PMC publication ID

9 September 2017

PMC publication ID

1 reference

Europe PubMed Central

PMC publication ID

9 September 2017

PubMed publication ID

1 reference

Europe PubMed Central

PMC publication ID

9 September 2017

ResearchGate publication ID

0 references

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