(Q39194678)

English

Characterization of the papain active centre by using two-protonic-state electrophiles as reactivity probes. Evidence for nucleophilic reactivity in the un-interrupted cysteine-25-histidine-159 interactive system

scientific article published on May 1, 1978

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scholarly article

1 reference

Europe PubMed Central

9 September 2017

Characterization of the papain active centre by using two-protonic-state electrophiles as reactivity probes. Evidence for nucleophilic reactivity in the un-interrupted cysteine-25-histidine-159 interactive system (English)

1 reference

10.1042/BJ1710385

https://api.crossref.org/works/10.1042/BJ1710385

24 October 2022

1 reference

10.1042/BJ1710385

https://api.crossref.org/works/10.1042/BJ1710385

24 October 2022

1 reference

10.1042/BJ1710385

https://api.crossref.org/works/10.1042/BJ1710385

24 October 2022

author name string

M. Shipton

1

1 reference

Europe PubMed Central

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A26335

24 October 2022

K. Brochlehurst

2

1 reference

Europe PubMed Central

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A26335

24 October 2022

language of work or name

0 references

publication date

1 May 1978

1 reference

Europe PubMed Central

9 September 2017

full work available at URL

https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/26335/?tool=EBI

online access status

1 reference

Europe PubMed Central

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A26335

24 October 2022

https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/26335/pdf/?tool=EBI

Portable Document Format

online access status

1 reference

Europe PubMed Central

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A26335

24 October 2022

https://europepmc.org/articles/PMC1183967

online access status

content deliverer

Europe PubMed Central

1 reference

Europe PubMed Central

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A26335

24 October 2022

https://europepmc.org/articles/PMC1183967?pdf=render

Portable Document Format

online access status

content deliverer

Europe PubMed Central

1 reference

Europe PubMed Central

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A26335

24 October 2022

https://portlandpress.com/biochemj/article-pdf/171/2/385/569721/bj1710385.pdf

Portable Document Format

1 reference

10.1042/BJ1710385

https://api.crossref.org/works/10.1042/BJ1710385

24 October 2022

Biochemical Journal

1 reference

Europe PubMed Central

9 September 2017

171

1 reference

Europe PubMed Central

9 September 2017

385-401

1 reference

Europe PubMed Central

9 September 2017

2

1 reference

Europe PubMed Central

9 September 2017

Tissue sulfhydryl groups

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1183967

10 September 2017

Structure of papain

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1183967

10 September 2017

The reaction of papain with Ellman's reagent (5,5'-dithiobis- (2-nitrobenzoate) dianion)

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1183967

10 September 2017

Specificity of induced conformational changes. Case of yeast glyceraldehyde-3-phosphate dehydrogenase

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1183967

10 September 2017

Intramolecular Inhibition by Enzyme of Site-Specific Modification Reactions can Mask pKa Values Characteristic of the Reaction Pathway: Do the Side Chains of Aspartic Acid-158 and Lysine-156 of Papain Form an Ion-Pair?

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1183967

10 September 2017

The pH-dependence of second-order rate constants of enzyme modification may provide free-reactant pKa values

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1183967

10 September 2017

Benzofuroxan as a thiol-specific reactivity probe. Kinetics of its reactions with papain, ficin, bromelain and low-molecular-weight thiols

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1183967

10 September 2017

Proton Equilibria in the Binding of Zn2+ and of Methylmercuric Iodide to Papain

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1183967

10 September 2017

4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole as a reactivity probe for the investigation of the thiol proteinases. evidence that ficin and bromelain may lack carboxyl groups conformationally equivalent to that of aspartic acid-158 of papain

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1183967

10 September 2017

Preparation of fully active ficin from Ficus glabrata by covalent chromatography and characterization of its active centre by using 2,2'-depyridyl disulphide as a reactivity probe

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1183967

10 September 2017

PH-dependence of the steady-state rate of a two-step enzymic reaction

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1183967

10 September 2017

A reporter group delivery system with both absolute and selective specificity for thiol groups and an improved fluorescent probe containing the 7-nitrobenzo-2-oxa-1,3-diazole moiety

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1183967

10 September 2017

The location of the active-site histidine residue in the primary sequence of papain

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1183967

15 June 2018

The amino acid sequence around the active-site cysteine and histidine residues, and the buried cysteine residue in ficin

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1183967

15 June 2018

Reactivities of the various protonic states in the reactions of papain and of L-cysteine with 2,2'- and with 4,4'- dipyridyl disulphide: evidence for nucleophilic reactivity in the un-ionized thiol group of the cysteine-25 residue of papain occasion

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1183967

15 June 2018

Covalent chromatography. Preparation of fully active papain from dried papaya latex

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1183967

15 June 2018

Shapes of curves of pH-dependence of reactions

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1183967

15 June 2018

Reactions of papain and of low-molecular-weight thiols with some aromatic disulphides. 2,2'-Dipyridyl disulphide as a convenient active-site titrant for papain even in the presence of other thiols

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1183967

15 June 2018

Mercaptide-imidazolium ion-pair: the reactive nucleophile in papain catalysis

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1183967

15 June 2018

Whither enzyme mechanisms?

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1183967

15 June 2018

Substituted Pyridines as Two-Protonic-State Reactivity Probes, Reporter-Group Delivery Vehicles and Labelling Reagents for the Study of Thiol Enzymes

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1183967

15 June 2018

The mutability of stem bromelain: evidence for perturbation by structural transitions of the parameters that characterize the reaction of the essential thiol group of bromelain with 2,2'-dipyridyl disulphide

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1183967

21 August 2018

Specificity of papain-catalyzed transamidation reactions

1 reference

https://pubmed.ncbi.nlm.nih.gov/26335

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

A novel reactivity of papain and a convenient active site titration in the presence of other thiols

1 reference

https://pubmed.ncbi.nlm.nih.gov/26335

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

On the mode of activation of the catalytically essential sulfhydryl group of papain

1 reference

https://pubmed.ncbi.nlm.nih.gov/26335

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Covalent chromatography by thiol-disulfide interchange

1 reference

https://pubmed.ncbi.nlm.nih.gov/26335

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Reaction of papain with -bromo- -(5-imidazolyl)propionic acid

1 reference

https://pubmed.ncbi.nlm.nih.gov/26335

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Determination of sulfhydryl groups with 2,2′- or 4,4′-dithiodipyridine

1 reference

https://pubmed.ncbi.nlm.nih.gov/26335

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Co-operative ionisation of aspartic-acid-158 and histidine-159 in papain. Evidence from 19F nuclear-magnetic-resonance and fluorescence spectroscopy

1 reference

https://pubmed.ncbi.nlm.nih.gov/26335

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Identifiers

10.1042/BJ1710385

1 reference

https://api.fatcat.wiki/v0/release/rnq34codyzfvnbbz3imeolbdwm

30 July 2022

based on heuristic

mapped directly with Wikidata item

release_rnq34codyzfvnbbz3imeolbdwm

1 reference

https://api.fatcat.wiki/v0/release/rnq34codyzfvnbbz3imeolbdwm

30 July 2022

based on heuristic

mapped directly with Wikidata item

1 reference

https://api.fatcat.wiki/v0/release/rnq34codyzfvnbbz3imeolbdwm

30 July 2022

based on heuristic

mapped directly with Wikidata item

1 reference

https://api.fatcat.wiki/v0/release/rnq34codyzfvnbbz3imeolbdwm

30 July 2022

based on heuristic

mapped directly with Wikidata item

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