(Q39524436)

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:21673940%20AND%20SRC:MED&resulttype=core&format=json

4 February 2020

title

Cloning, soluble expression and purification of high yield recombinant hGMCSF in Escherichia coli (English)

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:21673940%20AND%20SRC:MED&resulttype=core&format=json

4 February 2020

1 reference

Krishna Mohan Padmanabha Das

9

1 reference

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4 February 2020

author name string

Krishna M P Das

1

1 reference

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4 February 2020

Sampali Banerjee

2

1 reference

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4 February 2020

Nivedita Shekhar

3

1 reference

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4 February 2020

Karpagavalli Damodaran

4

1 reference

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4 February 2020

Rahul Nair

5

1 reference

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4 February 2020

Sandeep Somani

6

1 reference

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4 February 2020

Veena P Raiker

7

1 reference

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4 February 2020

Shweta Jain

8

1 reference

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4 February 2020

language of work or name

English

0 references

publication date

22 March 2011

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:21673940%20AND%20SRC:MED&resulttype=core&format=json

International Journal of Molecular Sciences

4 February 2020

published in

1 reference

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4 February 2020

volume

12

1 reference

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4 February 2020

issue

3

1 reference

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4 February 2020

page(s)

2064-2076

1 reference

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Creative Commons Attribution 4.0 International

4 February 2020

copyright license

start time

22 March 2011

1 reference

April 2022 Public Data File from Crossref

copyright status

copyrighted

0 references

cites work

High yielding recombinant Staphylokinase in bacterial expression system--cloning, expression, purification and activity studies

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3111651

Production of active eukaryotic proteins through bacterial expression systems: a review of the existing biotechnology strategies

12 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3111651

Select what you need: a comparative evaluation of the advantages and limitations of frequently used expression systems for foreign genes

12 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3111651

A family of E. coli expression vectors for laboratory scale and high throughput soluble protein production

12 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3111651

Advanced genetic strategies for recombinant protein expression in Escherichia coli

12 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3111651

Recombinant murine GM-CSF from E. coli has biological activity and is neutralized by a specific antiserum.

12 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3111651

Genomic cloning, characterization, and multilineage growth-promoting activity of human granulocyte-macrophage colony-stimulating factor.

12 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3111651

Increased biological activity of deglycosylated recombinant human granulocyte/macrophage colony-stimulating factor produced by yeast or animal cells

12 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3111651

Construction of intein-mediated hGMCSF expression vector and its purification in Pichia pastoris

12 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3111651

The presence of N-terminal secretion signal sequences leads to strong stimulation of the total expression levels of three tested medically important proteins during high-cell-density cultivations of Escherichia coli

14 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3111651

Human GM-CSF: molecular cloning of the complementary DNA and purification of the natural and recombinant proteins

17 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3111651

Expression of murine and human granulocyte-macrophage colony-stimulating factors in S. cerevisiae: mutagenesis of the potential glycosylation sites

17 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3111651

Molecular cloning, expression in Escherichia coli and production of bioactive homogeneous recombinant human granulocyte and macrophage colony stimulating factor

17 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3111651

Single step intein-mediated purification of hGMCSF expressed in salt-inducible E. coli

21 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3111651

The transcriptional response of Escherichia coli to recombinant protein insolubility.

21 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3111651

Recombinant protein expression and solubility screening in Escherichia coli: a comparative study.

21 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3111651

The solubility and stability of recombinant proteins are increased by their fusion to NusA.

21 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3111651

A recombinant human G-CSF/GM-CSF fusion protein from E. coli showing colony stimulating activity on human bone marrow cells.

21 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3111651

Purification and properties of bacterially synthesized human granulocyte-macrophage colony stimulating factor

21 August 2018

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/21673940

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

Monitoring of protein conformation by high-performance size-exclusion liquid chromatography and scanning diode array second-derivative UV absorption spectroscopy

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/21673940

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

Purification of recombinant human granulocyte-macrophage colony-stimulating factor from the inclusion bodies produced by transformed Escherichia coli cells

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/21673940

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

Identifiers

DOI

10.3390/IJMS12032064

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:21673940%20AND%20SRC:MED&resulttype=core&format=json

4 February 2020

0 references

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:21673940%20AND%20SRC:MED&resulttype=core&format=json

4 February 2020

PubMed publication ID

21673940

1 reference