(Q39572601)

13 September 2017

Directed evolution of green fluorescent protein by a new versatile PCR strategy for site-directed and semi-random mutagenesis (English)

1 reference

13 September 2017

0 references

0 references

A Sawano

1

1 reference

PMCID

108465

13 September 2017

A Miyawaki

series ordinal

2

1 reference

PMCID

108465

13 September 2017

language of work or name

English

0 references

publication date

1 August 2000

1 reference

13 September 2017

Nucleic Acids Research

1 reference

13 September 2017

28

1 reference

13 September 2017

E78

1 reference

13 September 2017

16

1 reference

Circular permutation and receptor insertion within green fluorescent proteins

13 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=108465

Dynamic and quantitative Ca2+ measurements using improved cameleons

14 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=108465

The green fluorescent protein

14 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=108465

Measurement of cytosolic, mitochondrial, and Golgi pH in single living cells with green fluorescent proteins

14 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=108465

An improved PCR-mutagenesis strategy for two-site mutagenesis or sequence swapping between related genes

14 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=108465

Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin

14 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=108465

Crystal structure of the Aequorea victoria green fluorescent protein

14 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=108465

Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer

14 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=108465

Wavelength mutations and posttranslational autoxidation of green fluorescent protein

14 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=108465

Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia

14 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=108465

Use of T7 RNA polymerase to direct expression of cloned genes

14 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=108465

Efficient site-directed mutagenesis using uracil-containing DNA.

14 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=108465

Engineering new functions and altering existing functions

14 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=108465

Improved green fluorescent protein by molecular evolution using DNA shuffling.

25 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=108465

Random mutagenesis by whole-plasmid PCR amplification

21 August 2018

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/10931937

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

Site-directed mutagenesis

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/10931937

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

Multiple site mutagenesis with high targeting efficiency in one cloning step

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/10931937

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

Identifiers

DOI

10.1093/NAR/28.16.E78

1 reference

13 September 2017

1 reference

13 September 2017

1 reference

PMCID

108465