(Q39778885)

14 September 2017

title

Carbon metabolism limits recombinant protein production in Pichia pastoris (English)

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14 September 2017

Pichia pastoris

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4

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14 September 2017

3

Lars M Blank

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14 September 2017

Jan Heyland

1

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14 September 2017

Jianan Fu

series ordinal

2

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14 September 2017

publication date

11 March 2011

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Biotechnology and Bioengineering

14 September 2017

published in

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14 September 2017

volume

108

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14 September 2017

issue

8

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14 September 2017

page(s)

1942-1953

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A multi-level study of recombinant Pichia pastoris in different oxygen conditions.

14 September 2017

cites work

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TCA cycle activity in Saccharomyces cerevisiae is a function of the environmentally determined specific growth and glucose uptake rates

21 January 2018

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Large-scale 13C-flux analysis reveals mechanistic principles of metabolic network robustness to null mutations in yeast

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Metabolic-flux and network analysis in fourteen hemiascomycetous yeasts

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Macromolecular and elemental composition analysis and extracellular metabolite balances of Pichia pastoris growing at different oxygen levels.

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Metabolic flux analysis for recombinant protein production by Pichia pastoris using dual carbon sources: Effects of methanol feeding rate

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Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris

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Recent advances in the expression of foreign genes in Pichia pastoris

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Recombinant protein expression in Pichia pastoris

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Theoretical growth yield estimates for recombinant cells

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Role of transcriptional regulation in controlling fluxes in central carbon metabolism of Saccharomyces cerevisiae. A chemostat culture study.

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Genome sequence of the recombinant protein production host Pichia pastoris

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The effect of temperature on the proteome of recombinant Pichia pastoris.

21 January 2018

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High-throughput metabolic flux analysis based on gas chromatography-mass spectrometry derived 13C constraints.

21 January 2018

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Monitoring of transcriptional regulation in Pichia pastoris under protein production conditions

21 January 2018

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A novel beta-peptidyl aminopeptidase (BapA) from strain 3-2W4 cleaves peptide bonds of synthetic beta-tri- and beta-dipeptides

21 January 2018

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Bacterial beta-peptidyl aminopeptidases with unique substrate specificities for beta-oligopeptides and mixed beta,alpha-oligopeptides

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Metabolic load and heterologous gene expression

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Amino acid supplementation, controlled oxygen limitation and sequential double induction improves heterologous xylanase production by Pichia stipitis

21 January 2018

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Amino acid supplementation improves heterologous protein production by Saccharomyces cerevisiae in defined medium

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Yeast systems biotechnology for the production of heterologous proteins

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Role of cultivation media in the development of yeast strains for large scale industrial use

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Promoter library designed for fine-tuned gene expression in Pichia pastoris

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Enzymatic degradation of beta- and mixed alpha,beta-oligopeptides

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Enzyme-catalyzed formation of beta-peptides: beta-peptidyl aminopeptidases BapA and DmpA acting as beta-peptide-synthesizing enzymes

21 January 2018

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Correlation between TCA cycle flux and glucose uptake rate during respiro-fermentative growth of Saccharomyces cerevisiae

21 January 2018

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Simple enzymatic procedure for L-carnosine synthesis: whole-cell biocatalysis and efficient biocatalyst recycling

21 January 2018

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Quantitative physiology of Pichia pastoris during glucose-limited high-cell density fed-batch cultivation for recombinant protein production

21 January 2018

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The effect of ethanol and acetate on protein expression in Pichia pastoris.

21 January 2018

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Pichia pastoris "just in time" alternative respiration

21 January 2018

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Condensed protocol for competent cell preparation and transformation of the methylotrophic yeast Pichia pastoris

21 January 2018

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Heterologous protein production using the Pichia pastoris expression system.

21 January 2018

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Stress in recombinant protein producing yeasts.

21 January 2018

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Genome, secretome and glucose transport highlight unique features of the protein production host Pichia pastoris.

21 January 2018

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Performance of the auxotrophic Saccharomyces cerevisiae BY4741 as host for the production of IL-1beta in aerated fed-batch reactor: role of ACA supplementation, strain viability, and maintenance energy

21 January 2018

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Do amino acid biosynthetic costs constrain protein evolution in Saccharomyces cerevisiae?

21 January 2018

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Limiting factors in Escherichia coli fed-batch production of recombinant proteins.

21 January 2018

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Escherichia coli physiology in Luria-Bertani broth

21 January 2018

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Getting started with yeast

21 January 2018

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Amino acid biosynthesis and metabolic flux profiling of Pichia pastoris

21 January 2018

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Metabolic flux profiling of Pichia pastoris grown on glycerol/methanol mixtures in chemostat cultures at low and high dilution rates

21 January 2018

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The role of ammonia metabolism in nitrogen catabolite repression in Saccharomyces cerevisiae

21 January 2018

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Isolation of the Pichia pastoris glyceraldehyde-3-phosphate dehydrogenase gene and regulation and use of its promoter

21 January 2018

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Metabolic adaptation of Escherichia coli during temperature-induced recombinant protein production: 2. Redirection of metabolic fluxes

21 January 2018

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Nutrient regulation of oligopeptide transport in Saccharomyces cerevisiae

21 January 2018

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FiatFlux--a software for metabolic flux analysis from 13C-glucose experiments

21 January 2018

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Constitutive expression of human angiostatin in Pichia pastoris by high-density cell culture

21 January 2018

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Recent advances on the GAP promoter derived expression system of Pichia pastoris

21 January 2018

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21 January 2018

Identifiers

DOI

10.1002/BIT.23114

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14 September 2017

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