(Q41337873)

29 September 2017

A simple, rapid, high-fidelity and cost-effective PCR-based two-step DNA synthesis method for long gene sequences (English)

1 reference

29 September 2017

Ai-Sheng Xiong

1

1 reference

29 September 2017

Quan-Hong Yao

2

1 reference

29 September 2017

Ri-He Peng

3

1 reference

29 September 2017

Xian Li

4

1 reference

29 September 2017

Hui-Qin Fan

5

1 reference

29 September 2017

Zong-Ming Cheng

6

1 reference

29 September 2017

Yi Li

7

1 reference

29 September 2017

7 July 2004

1 reference

29 September 2017

Nucleic Acids Research

1 reference

29 September 2017

32

1 reference

29 September 2017

12

1 reference

29 September 2017

e98

1 reference

Generating a synthetic genome by whole genome assembly: phiX174 bacteriophage from synthetic oligonucleotides

29 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=484193

Thermodynamically balanced inside-out (TBIO) PCR-based gene synthesis: a novel method of primer design for high-fidelity assembly of longer gene sequences

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=484193

Assembly of designed oligonucleotides as an efficient method for gene recombination: a new tool in directed evolution

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=484193

DNAWorks: an automated method for designing oligonucleotides for PCR-based gene synthesis

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=484193

Fidelity and mutational spectrum of Pfu DNA polymerase on a human mitochondrial DNA sequence

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=484193

Strategies for optimal synthesis and secretion of heterologous proteins in the methylotrophic yeast Pichia pastoris

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=484193

A synthetic cryIC gene, encoding a Bacillus thuringiensis delta-endotoxin, confers Spodoptera resistance in alfalfa and tobacco

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=484193

PCR fidelity of pfu DNA polymerase and other thermostable DNA polymerases

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=484193

Vip3A, a novel Bacillus thuringiensis vegetative insecticidal protein with a wide spectrum of activities against lepidopteran insects

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=484193

Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=484193

Chemical synthesis of a human interferon-alpha 2 gene and its expression in Escherichia coli

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=484193

Chemical synthesis and cloning of a gene for human β-urogastrone

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=484193

Codon usage in yeast: cluster analysis clearly differentiates highly and lowly expressed genes

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=484193

FokI method of gene synthesis

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=484193

Chemical synthesis of a gene for somatomedin C.

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=484193

Construction of synthetic genes using PCR after automated DNA synthesis of their entire top and bottom strands

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=484193

A delta-endotoxin encoded in Pseudomonas fluorescens displays a high degree of insecticidal activity

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=484193

Chemical synthesis and expression of a cassette adapted ubiquitin gene.

11 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=484193

Chemical synthesis, expression and product assessment of a gene coding for biologically active human tumour necrosis factor alpha.

11 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=484193

Recursive PCR: a novel technique for total gene synthesis

11 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=484193

Influence of signal peptide sequences on the expression of heterogeneous proteins in Pichia pastoris

11 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=484193

PCR-aided Synthesis and Stable Expression in E.coli of the cryIA (c)Bt Gene.

19 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=484193

[Synonymous codon usage in Pichia pastoris]

19 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=484193

Chemical synthesis of a biologically active gene for human immune interferon-gamma. Prospect for site-specific mutagenesis and structure-function studies

19 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=484193

Simultaneous mutagenesis of antibody CDR regions by overlap extension and PCR

19 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=484193

A rapid method for the construction of synthetic genes using the polymerase chain reaction

2 December 2018

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/15240836

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

[High expression of a heat-stable phytase in Pichia pastoris]

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/15240836

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

Identifiers

DOI

10.1093/NAR/GNH094

1 reference

29 September 2017

1 reference

29 September 2017

PubMed publication ID

15240836

1 reference