(Q31123994)

29 June 2017

Assembly of designed oligonucleotides as an efficient method for gene recombination: a new tool in directed evolution (English)

1 reference

29 June 2017

0 references

3

Manfred T Reetz

1 reference

29 June 2017

Dongxing Zha

1

1 reference

PubMed ID

12512074

29 June 2017

Andreas Eipper

series ordinal

2

1 reference

29 June 2017

1 January 2003

1 reference

29 June 2017

1 reference

29 June 2017

4

1 reference

29 June 2017

34-39

1 reference

29 June 2017

1

1 reference

Combinatorial and computational challenges for biocatalyst design

29 June 2017

1 reference

https://api.crossref.org/works/10.1002%2FCBIC.200390011

Directed Evolution and Biocatalysis

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FCBIC.200390011

Evolutionary optimisation of enzymes

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FCBIC.200390011

Investigating and Engineering Enzymes by Genetic Selection

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FCBIC.200390011

Cloning of random-sequence oligodeoxynucleotides

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FCBIC.200390011

Cassette mutagenesis: an efficient method for generation of multiple mutations at defined sites

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FCBIC.200390011

A new method for random mutagenesis of complete genes: enzymatic generation of mutant libraries in vitro.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FCBIC.200390011

DNA polymerase fidelity and the polymerase chain reaction

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FCBIC.200390011

Combinatorial cassette mutagenesis as a probe of the informational content of protein sequences

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FCBIC.200390011

Rapid insertional mutagenesis of DNA by polymerase chain reaction (PCR).

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FCBIC.200390011

Searching sequence space by definably random mutagenesis: improving the catalytic potency of an enzyme

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FCBIC.200390011

Creation of drug-specific herpes simplex virus type 1 thymidine kinase mutants for gene therapy

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FCBIC.200390011

Efficient gene targeted random mutagenesis in genetically stable Escherichia coli strains

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FCBIC.200390011

A method for directed evolution and functional cloning of enzymes

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FCBIC.200390011

Enantioselective enzymes for organic synthesis created by directed evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FCBIC.200390011

Inverting enantioselectivity by directed evolution of hydantoinase for improved production of L-methionine

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FCBIC.200390011

Directed evolution of D-2-keto-3-deoxy-6-phosphogluconate aldolase to new variants for the efficient synthesis of D- and L-sugars

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FCBIC.200390011

High-throughput screening for biocatalysts

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FCBIC.200390011

Synthetic lac operator DNA is functional in vivo

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FCBIC.200390011

Expression in Escherichia coli of a chemically synthesized gene for the hormone somatostatin

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FCBIC.200390011

Expression in Escherichia coli of chemically synthesized genes for human insulin

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FCBIC.200390011

FokI method of gene synthesis

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FCBIC.200390011

Recursive PCR: a novel technique for total gene synthesis

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FCBIC.200390011

Rapid evolution of a protein in vitro by DNA shuffling

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FCBIC.200390011

DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FCBIC.200390011

DNA shuffling of a family of genes from diverse species accelerates directed evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FCBIC.200390011

Analysis of shuffled gene libraries

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FCBIC.200390011

Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FCBIC.200390011

Molecular evolution by staggered extension process (StEP) in vitro recombination

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FCBIC.200390011

Directed evolution of proteins by exon shuffling

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FCBIC.200390011

Assembly of an active enzyme by the linkage of two protein modules

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FCBIC.200390011

Characteristics of super alphaA-crystallin, a product of in vitro exon shuffling

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FCBIC.200390011

Incremental truncation as a strategy in the engineering of novel biocatalysts

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FCBIC.200390011

Libraries of hybrid proteins from distantly related sequences

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FCBIC.200390011

DNA shuffling method for generating highly recombined genes and evolved enzymes

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FCBIC.200390011

Random insertion and deletion of arbitrary number of bases for codon-based random mutation of DNAs

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FCBIC.200390011

Gapped BLAST and PSI-BLAST: a new generation of protein database search programs

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FCBIC.200390011

A novel extracellular esterase from Bacillus subtilis and its conversion to a monoacylglycerol hydrolase.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FCBIC.200390011

Purification and preliminary characterization of the extracellular lipase of Bacillus subtilis 168, an extremely basic pH-tolerant enzyme

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FCBIC.200390011

A novel, high performance enzyme for starch liquefaction. Discovery and optimization of a low pH, thermostable alpha-amylase

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FCBIC.200390011

Directed evolution of a fungal peroxidase

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FCBIC.200390011

Directed Evolution of an Enantioselective Enzyme through Combinatorial Multiple-Cassette Mutagenesis

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FCBIC.200390011

Directed evolution of an enantioselective lipase

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FCBIC.200390011

Saturation mutagenesis using mixed oligonucleotides and M13 templates containing uracil

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FCBIC.200390011

Directed evolution of an esterase for the stereoselective resolution of a key intermediate in the synthesis of epothilones

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FCBIC.200390011

New methods for the high-throughput screening of enantioselective catalysts and biocatalysts

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FCBIC.200390011

Degenerate oligonucleotide gene shuffling (DOGS): a method for enhancing the frequency of recombination with family shuffling

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FCBIC.200390011

Finding Cinderella's slipper--proteins that fit

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FCBIC.200390011

An effective family shuffling method using single-stranded DNA

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FCBIC.200390011

A Method for High-Throughput Screening of Enantioselective Catalysts

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FCBIC.200390011

21 January 2018

Identifiers

DOI

10.1002/CBIC.200390011

1 reference

release_pmuxt3eo3feolislxbt434buv4

29 June 2017

1 reference

Fatcat

https://api.fatcat.wiki/v0/release/pmuxt3eo3feolislxbt434buv4

mapped directly with Wikidata item

24 November 2022

based on heuristic

PubMed ID

12512074

1 reference

PubMed ID

12512074