(Q41617749)

5 October 2017

title

3D versus 2D cell culture implications for electron microscopy (English)

1 reference

5 October 2017

Michael W Hess

1

1 reference

5 October 2017

Kristian Pfaller

2

1 reference

5 October 2017

Hannes L Ebner

3

1 reference

5 October 2017

Beate Beer

4

1 reference

5 October 2017

Daniel Hekl

5

1 reference

5 October 2017

Thomas Seppi

6

1 reference

5 October 2017

publication date

1 January 2010

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5 October 2017

published in

Methods in Cell Biology

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5 October 2017

volume

96

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5 October 2017

page(s)

649-670

1 reference

The development of necrosis and apoptosis in glioma: experimental findings using spheroid culture systems

5 October 2017

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Tissue architecture: the ultimate regulator of breast epithelial function

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Freeze-substitution: the addition of water to polar solvents enhances the retention of structure and acts at temperatures around -60 degrees C.

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7 January 2021

Morphogenesis and oncogenesis of MCF-10A mammary epithelial acini grown in three-dimensional basement membrane cultures

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7 January 2021

The hollow fibre model in cancer drug screening: the NCI experience

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7 January 2021

Adult rat cardiomyocytes cultured in creatine-deficient medium display large mitochondria with paracrystalline inclusions, enriched for creatine kinase

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7 January 2021

LLC-PK(1) cells maintained in a new perfusion cell culture system exhibit an improved oxidative metabolism

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7 January 2021

Spheroid-based drug screen: considerations and practical approach

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7 January 2021

Novel hybrid artificial liver using hepatocyte organoids

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7 January 2021

In vitro study of axonal migration and myelination of motor neurons in a three-dimensional tissue-engineered model

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7 January 2021

Microcarriers for high-pressure freezing and cryosectioning of adherent cells

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7 January 2021

Fusion pore expansion is a slow, discontinuous, and Ca2+-dependent process regulating secretion from alveolar type II cells

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7 January 2021

Cryopreparation methodology for plant cell biology.

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7 January 2021

Cryopreparation provides new insight into the effects of brefeldin A on the structure of the HepG2 Golgi apparatus

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7 January 2021

High-pressure freezing of tissue obtained by fine-needle biopsy

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7 January 2021

Studies on formalin fixation for electron microscopy and cytochemical staining purposes.

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7 January 2021

Three-dimensional cell organization leads to almost immediate HRE activity as demonstrated by molecular imaging of MG-63 spheroids using two-photon excitation microscopy

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7 January 2021

Aclar discs: a versatile substrate for routine high-pressure freezing of mammalian cell monolayers

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7 January 2021

Cell Culture on Artificial Capillaries: An Approach to Tissue Growth in vitro

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7 January 2021

Multicellular spheroids: a three-dimensional in vitro culture system to study tumour biology

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7 January 2021

Cdc42-dependent leading edge coordination is essential for interstitial dendritic cell migration

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7 January 2021

Three-dimensional cell culture matrices: state of the art.

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7 January 2021

Three-dimensional culture models of normal and malignant breast epithelial cells

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7 January 2021

Epoxy resin as fixative during freeze-substitution

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https://api.crossref.org/works/10.1016%2FS0091-679X%2810%2996027-5

7 January 2021

Cryomethods for thin section electron microscopy

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7 January 2021

Environment-mediated drug resistance: a major contributor to minimal residual disease

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7 January 2021

Cryopreparation of biological specimens for immunoelectron microscopy

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7 January 2021

High-pressure freezing in the study of animal pathogens.

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7 January 2021

The use of filter membranes for high‐pressure freezing of cell monolayers

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7 January 2021

Immunogold labeling of thawed cryosections

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7 January 2021

Chloroplast ultrastructure in leaves of Urtica dioica L. analyzed after high-pressure freezing and freeze-substitution and compared with conventional fixation followed by room temperature dehydration

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7 January 2021

Low O2 metabolism of HepG2 cells cultured at high density in a 3D microstructured scaffold

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7 January 2021

High-pressure freezing and low-temperature fixation of cell monolayers grown on sapphire coverslips

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2810%2996027-5

7 January 2021

Cryo-section immunolabelling of difficult to preserve specimens: advantages of cryofixation, freeze-substitution and rehydration

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2810%2996027-5

7 January 2021

Loss-of-function of MYO5B is the main cause of microvillus inclusion disease: 15 novel mutations and a CaCo-2 RNAi cell model.

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2810%2996027-5

7 January 2021

Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2810%2996027-5

7 January 2021

High-pressure freezing of isolated gastric glands provides new insight into the fine structure and subcellular localization of H+/K+-ATPase in gastric parietal cells

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2810%2996027-5

7 January 2021

Correlative light and electron microscopy using immunolabeled resin sections

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2810%2996027-5

7 January 2021

On optimizing high-pressure freezing: from heat transfer theory to a new microbiopsy device

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7 January 2021

Life isn't flat: taking cancer biology to the next dimension

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7 January 2021

The combination of chemical fixation procedures with high pressure freezing and freeze substitution preserves highly labile tissue ultrastructure for electron tomography applications

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https://api.crossref.org/works/10.1016%2FS0091-679X%2810%2996027-5

7 January 2021

A low-viscosity epoxy resin embedding medium for electron microscopy

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https://api.crossref.org/works/10.1016%2FS0091-679X%2810%2996027-5

7 January 2021

Cell and environment interactions in tumor microregions: the multicell spheroid model

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https://api.crossref.org/works/10.1016%2FS0091-679X%2810%2996027-5

7 January 2021

A technique for ultracryotomy of cell suspensions and tissues

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7 January 2021

Controlled microaspiration for high-pressure freezing: a new method for ultrastructural preservation of fragile and sparse tissues for TEM and electron tomography

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2810%2996027-5

7 January 2021

Immunogold labeling of cryosections from high-pressure frozen cells

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2810%2996027-5

7 January 2021

ELECTRON MICROSCOPY AFTER RAPID FREEZING ON A METAL SURFACE AND SUBSTITUTION FIXATION

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https://api.crossref.org/works/10.1016%2FS0091-679X%2810%2996027-5

7 January 2021

A rapid microbiopsy system to improve the preservation of biological samples prior to high‐pressure freezing

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2810%2996027-5

7 January 2021

Freeze substitution of high-pressure frozen samples: the visibility of biological membranes is improved when the substitution medium contains water

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2810%2996027-5

7 January 2021

Unraveling the microenvironmental influences on the normal mammary gland and breast cancer

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2810%2996027-5

7 January 2021

The significance of the Golgi complex in envelopment of bovine herpesvirus 1 (BHV-1) as revealed by cryobased electron microscopy

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2810%2996027-5

7 January 2021

Isolation and characterization of cancer stem cells from a human glioblastoma cell line U87.

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2810%2996027-5

7 January 2021

Immuno-electron tomography of ER exit sites reveals the existence of free COPII-coated transport carriers.

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2810%2996027-5

7 January 2021

Effects of cadmium on electrolyte ions in cultured rat hepatocytes studied by X-ray microanalysis of cryosections

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2810%2996027-5

7 January 2021

Heavy metal cytotoxicity studied by electron probe X-ray microanalysis of cultured rat hepatocytes

1 reference

https://api.crossref.org/works/10.1016%2FS0091-679X%2810%2996027-5

7 January 2021

10.1016/S0091-679X(10)96027-5

Identifiers

DOI

1 reference

5 October 2017

1 reference