(Q42051325)

English

Fluorescent-increase kinetics of different fluorescent reporters used for qPCR depend on monitoring chemistry, targeted sequence, type of DNA input and PCR efficiency.

scientific article published on 14 January 2014

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scholarly article

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Europe PubMed Central

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:25253910%20AND%20SRC:MED&resulttype=core&format=json

19 February 2020

Fluorescent-increase kinetics of different fluorescent reporters used for qPCR depend on monitoring chemistry, targeted sequence, type of DNA input and PCR efficiency (English)

1 reference

Europe PubMed Central

PMC publication ID

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:25253910%20AND%20SRC:MED&resulttype=core&format=json

19 February 2020

4

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Europe PubMed Central

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:25253910%20AND%20SRC:MED&resulttype=core&format=json

19 February 2020

1

object named as

Jan M Ruijter

1 reference

Europe PubMed Central

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19 February 2020

author name string

Peter Lorenz

2

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Europe PubMed Central

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:25253910%20AND%20SRC:MED&resulttype=core&format=json

19 February 2020

Jari M Tuomi

3

1 reference

Europe PubMed Central

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:25253910%20AND%20SRC:MED&resulttype=core&format=json

19 February 2020

Maurice J B van den Hoff

5

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Europe PubMed Central

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19 February 2020

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publication date

14 January 2014

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Europe PubMed Central

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19 February 2020

Microchimica Acta

1 reference

Europe PubMed Central

PMC publication ID

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:25253910%20AND%20SRC:MED&resulttype=core&format=json

19 February 2020

181

1 reference

Europe PubMed Central

PMC publication ID

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:25253910%20AND%20SRC:MED&resulttype=core&format=json

19 February 2020

13-14

1 reference

Europe PubMed Central

PMC publication ID

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:25253910%20AND%20SRC:MED&resulttype=core&format=json

19 February 2020

1689-1696

1 reference

Europe PubMed Central

PMC publication ID

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:25253910%20AND%20SRC:MED&resulttype=core&format=json

19 February 2020

copyright license

Creative Commons Attribution 2.0 Generic

14 January 2014

1 reference

April 2022 Public Data File from Crossref

copyright status

0 references

MNAzyme qPCR with superior multiplexing capacity

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4167442

5 June 2018

Evaluation of qPCR curve analysis methods for reliable biomarker discovery: bias, resolution, precision, and implications

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4167442

5 June 2018

The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4167442

5 June 2018

Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR data

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4167442

5 June 2018

Diagnosis and monitoring of PML-RARalpha-positive acute promyelocytic leukemia by quantitative RT-PCR.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4167442

5 June 2018

Basic principles of real-time quantitative PCR.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4167442

5 June 2018

Multiplex quantitative PCR using self-quenched primers labeled with a single fluorophore

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4167442

5 June 2018

Light-up probes: thiazole orange-conjugated peptide nucleic acid for detection of target nucleic acid in homogeneous solution.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4167442

5 June 2018

Detection of PCR products using self-probing amplicons and fluorescence

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4167442

5 June 2018

Molecular beacons: probes that fluoresce upon hybridization

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4167442

5 June 2018

A closed tube format for amplification and detection of DNA based on energy transfer

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4167442

5 June 2018

Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4167442

5 June 2018

The ongoing evolution of qPCR.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4167442

17 August 2018

Bias in the Cq value observed with hydrolysis probe based quantitative PCR can be corrected with the estimated PCR efficiency value.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4167442

17 August 2018

Quantitation of gene expression in neural precursors by reverse-transcription polymerase chain reaction using self-quenched, fluorogenic primers

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4167442

17 August 2018

Design and optimization of reverse-transcription quantitative PCR experiments

1 reference

https://api.crossref.org/works/10.1007%2FS00604-013-1155-8

21 January 2018

Interactive fluorophore and quencher pairs for labeling fluorescent nucleic acid hybridization probes

1 reference

https://pubmed.ncbi.nlm.nih.gov/25253910

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Continuous fluorescence monitoring of rapid cycle DNA amplification

1 reference

https://pubmed.ncbi.nlm.nih.gov/25253910

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

The limit of PCR amplification

1 reference

https://pubmed.ncbi.nlm.nih.gov/25253910

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Detection of telomerase activity utilizing energy transfer primers: comparison with gel- and ELISA-based detection

1 reference

https://pubmed.ncbi.nlm.nih.gov/25253910

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Identifiers

10.1007/S00604-013-1155-8

1 reference

Europe PubMed Central

PMC publication ID

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:25253910%20AND%20SRC:MED&resulttype=core&format=json

19 February 2020

PMC publication ID

1 reference

Europe PubMed Central

PMC publication ID

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:25253910%20AND%20SRC:MED&resulttype=core&format=json

19 February 2020

PubMed publication ID

1 reference

Europe PubMed Central

PMC publication ID

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:25253910%20AND%20SRC:MED&resulttype=core&format=json

19 February 2020

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