(Q42906133)

10 November 2017

Efficient protein production method for NMR using soluble protein tags with cold shock expression vector (English)

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10 November 2017

Chojiro Kojima

2

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author name string

Kokoro Hayashi

series ordinal

1

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10 November 2017

16 September 2010

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Journal of Biomolecular NMR

10 November 2017

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10 November 2017

48

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10 November 2017

3

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10 November 2017

147-155

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https://scigraph.springernature.com/pub.10.1007/s10858-010-9445-5

10 November 2017

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cites work

CspA, the major cold shock protein of Escherichia coli, negatively regulates its own gene expression

1 reference

Crossref

https://api.crossref.org/works/10.1007%2FS10858-010-9445-5

The structure and function of proline-rich regions in proteins

21 January 2018

1 reference

Crossref

https://api.crossref.org/works/10.1007%2FS10858-010-9445-5

Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase

21 January 2018

1 reference

12 December 2020

Enhancement of soluble protein expression through the use of fusion tags

1 reference

12 December 2020

Strategies for achieving high-level expression of genes in Escherichia coli

1 reference

12 December 2020

A new general method for the biosynthesis of stable isotope-enriched peptides using a decahistidine-tagged ubiquitin fusion system: an application to the production of mastoparan-X uniformly enriched with 15N and 15N/13C.

1 reference

12 December 2020

Attachment of an NMR-invisible solubility enhancement tag using a sortase-mediated protein ligation method

1 reference

12 December 2020

Efficient and rapid affinity purification of proteins using recombinant fusion proteases

1 reference

12 December 2020

Highly efficient expression and purification system of small-size protein domains in Escherichia coli for biochemical characterization.

1 reference

12 December 2020

A solubility-enhancement tag (SET) for NMR studies of poorly behaving proteins.

1 reference

12 December 2020

Translational enhancement by an element downstream of the initiation codon in Escherichia coli

1 reference

12 December 2020

Increase of solubility of foreign proteins in Escherichia coli by coproduction of the bacterial thioredoxin

1 reference

12 December 2020

Comparison of SUMO fusion technology with traditional gene fusion systems: enhanced expression and solubility with SUMO.

1 reference

12 December 2020

Peptidase D of Escherichia coli K-12, a metallopeptidase of low substrate specificity.

1 reference

12 December 2020

NMR spectroscopy as a tool for the rapid assessment of the conformation of GST-fusion proteins

1 reference

12 December 2020

Substrate requirements of human rhinovirus 3C protease for peptide cleavage in vitro

1 reference

12 December 2020

Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein

1 reference

12 December 2020

Cold-shock induced high-yield protein production in Escherichia coli

1 reference

12 December 2020

Nonsense mutations in cspA cause ribosome trapping leading to complete growth inhibition and cell death at low temperature in Escherichia coli

1 reference

12 December 2020

Using codon optimization, chaperone co-expression, and rational mutagenesis for production and NMR assignments of human eIF2 alpha.

1 reference

12 December 2020

1H, 13C and 15N resonance assignments of the VAP-A: OSBP complex

1 reference

12 December 2020

Deletion analysis of cspA of Escherichia coli: requirement of the AT-rich UP element for cspA transcription and the downstream box in the coding region for its cold shock induction

1 reference

12 December 2020

Solution structure of the cytoplasmic region of Na+/H+ exchanger 1 complexed with essential cofactor calcineurin B homologous protein 1.

1 reference

12 December 2020

pCold-GST vector: a novel cold-shock vector containing GST tag for soluble protein production

1 reference

12 December 2020

Electrostatic interaction between oxysterol-binding protein and VAMP-associated protein A revealed by NMR and mutagenesis studies

1 reference

12 December 2020

Complete growth inhibition of Escherichia coli by ribosome trapping with truncated cspA mRNA at low temperature.

1 reference

12 December 2020

10.1007/S10858-010-9445-5

Identifiers

DOI

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PubMed ID

20844927

Dimensions Publication ID

10 November 2017

0 references

1 reference

PubMed ID

20844927