(Q43078051)

13 November 2017

Contribution of active site residues to the activity and thermal stability of ribonuclease Sa (English)

1 reference

site-directed mutagenesis

12 March 2024

main subject

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A14500895

12 March 2024

1 reference

12 March 2024

2

Vladimir A. Mitkevich

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A14500895

12 March 2024

author name string

Saul Trevino

4

1 reference

13 November 2017

Stephanie Newsom

5

1 reference

13 November 2017

Gennady I. Yakovlev

1

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A14500895

12 March 2024

Kevin L. Shaw

3

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A14500895

12 March 2024

C. Nick Pace

6

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A14500895

12 March 2024

Alexander A. Makarov

7

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A14500895

12 March 2024

language of work or name

English

1 reference

National Center for Biotechnology Information

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/esummary.fcgi?db=pubmed&retmode=json&id=14500895

12 March 2024

publication date

1 October 2003

1 reference

full work available at URL

13 November 2017

https://europepmc.org/articles/pmc2366910?pdf=render

file format

Portable Document Format

online access status

open access

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A14500895

https://europepmc.org/articles/PMC2366910

12 March 2024

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A14500895

https://europepmc.org/articles/PMC2366910?pdf=render

12 March 2024

file format

Portable Document Format

online access status

open access

content deliverer

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A14500895

https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1110%2Fps.03176803

12 March 2024

1 reference

https://onlinelibrary.wiley.com/doi/pdf/10.1110/ps.03176803

12 March 2024

1 reference

12 March 2024

1 reference

13 November 2017

12

1 reference

13 November 2017

10

1 reference

13 November 2017

2367-2373

1 reference

pK values of histidine residues in ribonuclease Sa: effect of salt and net charge.

13 November 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

Impact of enzyme motion on activity

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

Kinetic studies of guanine recognition and a phosphate group subsite on ribonuclease T1 using substitution mutants at Glu46 and Lys41.

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

Structural bases of stability-function tradeoffs in enzymes

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

Atomic resolution data reveal flexibility in the structure of RNase Sa

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

Prediction of functionally important residues based solely on the computed energetics of protein structure.

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

Tyrosine hydrogen bonds make a large contribution to protein stability

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

Solution structure and dynamics of ribonuclease Sa

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

The effect of net charge on the solubility, activity, and stability of ribonuclease Sa

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

Polar group burial contributes more to protein stability than nonpolar group burial

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

Buried charged surface in proteins

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

Charge-charge interactions influence the denatured state ensemble and contribute to protein stability

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

Binding of bisubstrate analog promotes large structural changes in the unregulated catalytic trimer of aspartate transcarbamoylase: Implications for allosteric regulation

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

Increasing protein stability by altering long-range coulombic interactions

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

Contribution of a conserved asparagine to the conformational stability of ribonucleases Sa, Ba, and T1

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

A decade of protein engineering on ribonuclease T1--atomic dissection of the enzyme-substrate interactions

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

Complex of ribonuclease Sa with a cyclic nucleotide and a proposed model for the reaction intermediate

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

A relationship between protein stability and protein function

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

The role of Glu-60 in the specificity of the recombinant ribonuclease from Bacillus amyloliquefaciens (barnase) towards dinucleotides, poly(A) and RNA

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

The interpretation of protein structures: Total volume, group volume distributions and packing density

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

Quantitative analysis of the contribution of Glu46 and Asn98 to the guanosine specificity of ribonuclease T1.

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

His92Ala mutation in ribonuclease T1 induces segmental flexibility. An X-ray study

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

Energetics of enzyme catalysis

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

Subsites and catalytic mechanism of ribonuclease T1: kinetic studies using GpA, GpC, GpG, and GpU as substrates

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

Subsites and catalytic mechanism of ribonuclease T1: Kinetic studies using GpC and GpU as substrates

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

Expression of Bacillus amyloliquefaciens extracellular ribonuclease (barnase) in Escherichia coli following an inactivating mutation.

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

Charge-charge interactions are key determinants of the pK values of ionizable groups in ribonuclease Sa (pI=3.5) and a basic variant (pI=10.2).

25 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

Analysis of catalytic residues in enzyme active sites

15 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

Electrostatic origin of the catalytic power of enzymes and the role of preorganized active sites

15 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

Conformational stability and thermodynamics of folding of ribonucleases Sa, Sa2 and Sa3.

15 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

Functional Interactions among the His40, Glu58 and His92 Catalysts of Ribonuclease T1 as Studied by Double and Triple Mutants

15 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2366910

The number of cooperative regions (energetic domains) in a pepsin molecule depends on the pH of the medium

1 December 2018

1 reference

Crossref

https://api.crossref.org/works/10.1110%2FPS.03176803

Determination and restrained least-squares refinement of the structures of ribonuclease Sa and its complex with 3'-guanylic acid at 1.8 Å resolution

21 January 2018

1 reference

Crossref

https://api.crossref.org/works/10.1110%2FPS.03176803

21 January 2018

1 reference

https://api.crossref.org/works/10.1110%2FPS.03176803

A comparative study on the catalytic properties of guanyl-specific ribonucleases

21 January 2018

1 reference

12 December 2020

Effect of active site residues in barnase on activity and stability

1 reference

12 December 2020

PHYSICAL AND CHEMICAL CHARACTERIZATION OF THE ORDERED COMPLEXES FORMED BETWEEN POLYINOSINIC ACID, POLYCYTIDYLIC ACID AND THEIR DEOXYRIBO-ANALOGUES

1 reference

12 December 2020

Ribonuclease from Streptomyces aureofaciens at atomic resolution

1 reference

12 December 2020

Purification of ribonucleases Sa, Sa2, and Sa3 after expression in Escherichia coli

1 reference

12 December 2020

Contribution of arginine-82 and arginine-86 to catalysis of RNases from Bacillus intermedius (binase)

1 reference

12 December 2020

Specific protein-nucleic acid recognition in ribonuclease T1–2′-guanylic acid complex: an X-ray study

1 reference

12 December 2020

The structural and sequence homology of a family of microbial ribonucleases

13

1 reference

Ribonuclease T1: Structure, Function, and Stability

12 March 2024

23

1 reference

Cloning, sequencing and transcription of an inactivated copy of Bacillus amyloliquefaciens extracellular ribonuclease (barnase).

12 March 2024

27

1 reference

12 March 2024

Identifiers

DOI

10.1110/PS.03176803

1 reference

13 November 2017

1 reference

13 November 2017

1 reference