(Q44038146)

29 November 2017

Production of functional single-chain Fv antibodies in the cytoplasm of Escherichia coli (English)

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29 November 2017

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4

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29 November 2017

5

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29 November 2017

Paola Jurado

1

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author name string

Daniel Ritz

series ordinal

2

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PubMed ID

12079330

29 November 2017

Jon Beckwith

series ordinal

3

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PubMed ID

12079330

29 November 2017

language of work or name

English

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publication date

1 June 2002

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Journal of Molecular Biology

29 November 2017

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29 November 2017

320

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29 November 2017

1

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29 November 2017

1-10

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Engineering antibodies for imaging and therapy

29 November 2017

1 reference

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The use of recombinant antibodies in proteomics

7 January 2021

1 reference

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Making antibodies by phage display technology

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2900405-9

The immunoglobulin superfamily--domains for cell surface recognition

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2900405-9

Stability engineering of antibody single-chain Fv fragments

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2900405-9

The disulfide bonds in antibody variable domains: effects on stability, folding in vitro, and functional expression in Escherichia coli.

7 January 2021

1 reference

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Mutual Stabilization of VLand VHin Single-Chain Antibody Fragments, Investigated with Mutants Engineered for Stability†

7 January 2021

1 reference

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An intrinsically stable antibody scFv fragment can tolerate the loss of both disulfide bonds and fold correctly.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2900405-9

A natural antibody missing a cysteine in VH: consequences for thermodynamic stability and folding.

7 January 2021

1 reference

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Antibody scFv fragments without disulfide bonds made by molecular evolution

7 January 2021

1 reference

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Intracellular antibodies: development and therapeutic potential.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2900405-9

The selection of intracellular antibodies.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2900405-9

Protein folding in the bacterial periplasm

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2900405-9

Making and breaking disulfide bonds

7 January 2021

1 reference

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The genetics of disulfide bond metabolism

7 January 2021

1 reference

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The role of the thioredoxin and glutaredoxin pathways in reducing protein disulfide bonds in the Escherichia coli cytoplasm.

7 January 2021

1 reference

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Roles of thiol-redox pathways in bacteria

7 January 2021

1 reference

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Functional expression of a single-chain anti-progesterone antibody fragment in the cytoplasm of a mutant Escherichia coli

7 January 2021

1 reference

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Functional antibody single-chain fragments from the cytoplasm of Escherichia coli: influence of thioredoxin reductase (TrxB).

7 January 2021

1 reference

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Mutations that allow disulfide bond formation in the cytoplasm of Escherichia coli.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2900405-9

Expression of an antibody fragment at high levels in the bacterial cytoplasm.

7 January 2021

1 reference

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Escherichia coli maltose-binding protein as a molecular chaperone for recombinant intracellular cytoplasmic single-chain antibodies.

7 January 2021

1 reference

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Efficient folding of proteins with multiple disulfide bonds in the Escherichia coli cytoplasm

7 January 2021

1 reference

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Tightly regulated tac promoter vectors useful for the expression of unfused and fused proteins in Escherichia coli.

7 January 2021

1 reference

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Determinants of membrane protein topology

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2900405-9

Identification of a protein required for disulfide bond formation in vivo.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2900405-9

Specific secretion of active single-chain Fv antibodies into the supernatants of Escherichia coli cultures by use of the hemolysin system

7 January 2021

1 reference

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Respiratory chain is required to maintain oxidized states of the DsbA-DsbB disulfide bond formation system in aerobically growing Escherichia coli cells

7 January 2021

1 reference

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Mitochondria can import artificial precursor proteins containing a branched polypeptide chain or a carboxy-terminal stilbene disulfonate

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2900405-9

Importance of redox potential for the in vivo function of the cytoplasmic disulfide reductant thioredoxin from Escherichia coli

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2900405-9

Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter

7 January 2021

1 reference

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Monitoring intracellular levels of XylR in Pseudomonas putida with a single-chain antibody specific for aromatic-responsive enhancer-binding proteins.

7 January 2021

1 reference

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Kabat Database and its applications: future directions

7 January 2021

1 reference

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Kabat Database and its applications: 30 years after the first variability plot

7 January 2021

1 reference

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Refolding of bovine pancreatic trypsin inhibitor via non-native disulphide intermediates

7 January 2021

1 reference

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Stability of wild-type and mutant RTEM-1 beta-lactamases: effect of the disulfide bond

7 January 2021

1 reference

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Kinetic and thermodynamic consequences of the removal of the Cys-77-Cys-123 disulphide bond for the folding of TEM-1 beta-lactamase

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2900405-9

Disulfide bond formation in the Escherichia coli cytoplasm: an in vivo role reversal for the thioredoxins

7 January 2021

1 reference

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Conversion of a peroxiredoxin into a disulfide reductase by a triplet repeat expansion

7 January 2021

1 reference

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An in vivo pathway for disulfide bond isomerization in Escherichia coli

7 January 2021

1 reference

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Structural and functional characterization of DsbC, a protein involved in disulfide bond formation in Escherichia coli.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2900405-9

Contributions of substrate binding to the catalytic activity of DsbC.

7 January 2021

1 reference

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High level production of functional antibody Fab fragments in an oxidizing bacterial cytoplasm

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2900405-9

Production of correctly folded Fab antibody fragment in the cytoplasm of Escherichia coli trxB gor mutants via the coexpression of molecular chaperones

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2900405-9

Different Equilibrium Stability Behavior of ScFv Fragments: Identification, Classification, and Improvement by Protein Engineering†

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2900405-9

Screening of phage antibody libraries

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2900405-9

Intracellular immunization of prokaryotic cells against a bacteriotoxin

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2900405-9

inferred from DOI database lookup

7 January 2021

based on heuristic

Identifiers

DOI

10.1016/S0022-2836(02)00405-9

1 reference

29 November 2017

1 reference

PubMed ID

12079330