(Q44498521)

3 December 2017

title

Differential effects of supplementary affinity tags on the solubility of MBP fusion proteins (English)

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3 December 2017

Karen M Routzahn

1

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3 December 2017

David S Waugh

series ordinal

2

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3 December 2017

publication date

1 January 2002

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Journal of Structural and Functional Genomics

3 December 2017

published in

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3 December 2017

volume

2

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3 December 2017

issue

2

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3 December 2017

page(s)

83-92

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https://scigraph.springernature.com/pub.10.1023/a:1020424023207

3 December 2017

exact match

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cites work

Protein production: feeding the crystallographers and NMR spectroscopists

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12 December 2020

The Protein Data Bank

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Release of proteins and peptides from fusion proteins using a recombinant plant virus proteinase

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Location of tolerated insertions/deletions in the structure of the maltose binding protein

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The 2.3-A resolution structure of the maltose- or maltodextrin-binding protein, a primary receptor of bacterial active transport and chemotaxis

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12 December 2020

Structural basis for oligosaccharide recognition by Pyrococcus furiosus maltodextrin-binding protein

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12 December 2020

Expression of heterologous peptides at two permissive sites of the MalE protein: antigenicity and immunogenicity of foreign B-cell and T-cell epitopes

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12 December 2020

Use of peptide libraries to map the substrate specificity of a peptide-modifying enzyme: a 13 residue consensus peptide specifies biotinylation in Escherichia coli.

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12 December 2020

Escherichia coli maltose-binding protein as a molecular chaperone for recombinant intracellular cytoplasmic single-chain antibodies.

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12 December 2020

Immobilized nitro-avidin and nitro-streptavidin as reusable affinity matrices for application in avidin-biotin technology

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12 December 2020

Single amino acid substitutions on the surface of Escherichia coli maltose-binding protein can have a profound impact on the solubility of fusion proteins

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12 December 2020

Differential effects of short affinity tags on the crystallization of Pyrococcus furiosus maltodextrin-binding protein

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12 December 2020

66 Analysis of microbial biotin proteins

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12 December 2020

High-level expression of soluble protein in Escherichia coli using a His6-tag and maltose-binding-protein double-affinity fusion system.

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12 December 2020

Creating a bifunctional protein by insertion of beta-lactamase into the maltodextrin-binding protein

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12 December 2020

Design of high-throughput methods of protein production for structural biology

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12 December 2020

A versatile plasmid expression vector for the production of biotinylated proteins by site-specific, enzymatic modification in Escherichia coli

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12 December 2020

A minimal peptide substrate in biotin holoenzyme synthetase-catalyzed biotinylation

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12 December 2020

Monovalent avidin affinity columns

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12 December 2020

Site-directed mutagenesis by overlap extension using the polymerase chain reaction

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12 December 2020

Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused

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12 December 2020

Recombinant protein expression in Escherichia coli

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12 December 2020

Identifiers

DOI

10.1023/A:1020424023207

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Dimensions Publication ID

3 December 2017

1043167466

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