(Q44577978)

English

Cloning, expression, and purification of the His6-tagged hyper-thermostable dUTPase from Pyrococcus woesei in Escherichia coli: application in PCR.

scientific article published in September 2003

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scholarly article

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Europe PubMed Central

PubMed publication ID

4 December 2017

Cloning, expression, and purification of the His6-tagged hyper-thermostable dUTPase from Pyrococcus woesei in Escherichia coli: application in PCR. (English)

1 reference

Europe PubMed Central

PubMed publication ID

4 December 2017

Escherichia coli

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Pyrococcus woesei

object named as

Pyrococcus woesei

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author name string

Sławomir Dabrowski

1

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Europe PubMed Central

PubMed publication ID

4 December 2017

Birgitte Kiaer Ahring

2

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Europe PubMed Central

PubMed publication ID

4 December 2017

publication date

1 September 2003

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Europe PubMed Central

PubMed publication ID

4 December 2017

Protein Expression and Purification

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Europe PubMed Central

PubMed publication ID

4 December 2017

31

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Europe PubMed Central

PubMed publication ID

4 December 2017

1

1 reference

Europe PubMed Central

PubMed publication ID

4 December 2017

72-78

1 reference

Europe PubMed Central

PubMed publication ID

4 December 2017

Phosphorothioate primers improve the amplification of DNA sequences by DNA polymerases with proofreading activity

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Low Incorporation of dUMP by Some Thermostable DNA Polymerases May Limit Their Use in PCR Amplifications

1 reference

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Archaebacterial DNA polymerases tightly bind uracil-containing DNA.

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Structural basis for uracil recognition by archaeal family B DNA polymerases

1 reference

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Archaeal dUTPase enhances PCR amplifications with archaeal DNA polymerases by preventing dUTP incorporation

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Lethality of a dut (deoxyuridine triphosphatase) mutation in Escherichia coli

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Effects of rare codon clusters on high-level expression of heterologous proteins in Escherichia coli

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Errors of heterologous protein expression

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Consecutive low-usage leucine codons block translation only when near the 5' end of a message in Escherichia coli.

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Purification of recombinant proteins with metal chelate adsorbent

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Cloning, overexpression, and purification of the recombinant His-tagged SSB protein of Escherichia coli and use in polymerase chain reaction amplification

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Cloning, expression, and purification of the His(6)-tagged thermostable beta-galactosidase from Pyrococcus woesei in Escherichia coli and some properties of the isolated enzyme

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Immobilized metal ion affinity adsorption and immobilized metal ion affinity chromatography of biomaterials. Serum protein affinities for gel-immobilized iron and nickel ions

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Cloning and expression in Escherichia coli of the recombinant his-tagged DNA polymerases from Pyrococcus furiosus and Pyrococcus woesei

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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

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Identifiers

10.1016/S1046-5928(03)00108-6

1 reference

Europe PubMed Central

PubMed publication ID

4 December 2017

PubMed publication ID

1 reference

Europe PubMed Central

PubMed publication ID

4 December 2017

ResearchGate publication ID

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