(Q44720075)

5 December 2017

title

Principles and methods of affinity cleavage in studying transcription (English)

1 reference

5 December 2017

Claude F Meares

1

1 reference

5 December 2017

Saul A Datwyler

2

1 reference

5 December 2017

Brian D Schmidt

3

1 reference

5 December 2017

Jeffrey Owens

4

1 reference

5 December 2017

Akira Ishihama

5

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5 December 2017

language of work or name

English

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publication date

1 January 2003

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5 December 2017

published in

Methods in Enzymology

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5 December 2017

volume

371

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5 December 2017

page(s)

82-106

1 reference

Map of distamycin, netropsin, and actinomycin binding sites on heterogeneous DNA: DNA cleavage-inhibition patterns with methidiumpropyl-EDTA.Fe(II)

5 December 2017

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7 January 2021

Sequence-specific double-strand cleavage of DNA by penta-N-methylpyrrolecarboxamide-EDTA X Fe(II).

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7 January 2021

Hydroxyl radical "footprinting": high-resolution information about DNA-protein contacts and application to lambda repressor and Cro protein

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7 January 2021

Sequence-specific cleavage of single-stranded DNA: oligodeoxynucleotide-EDTA X Fe(II).

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7 January 2021

Mapping protein domains involved in macromolecular interactions: a novel protein footprinting approach.

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7 January 2021

Synthesis of the protein cutting reagent iron (S)-1-(p-bromoacetamidobenzyl)ethylenediaminetetraacetate and conjugation to cysteine side chains

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7 January 2021

Transfer of oxygen from an artificial protease to peptide carbon during proteolysis

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7 January 2021

Conformation-dependent cleavage of staphylococcal nuclease with a disulfide-linked iron chelate

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7 January 2021

Incorporation of an EDTA-metal complex at a rationally selected site within a protein: application to EDTA-iron DNA affinity cleaving with catabolite gene activator protein (CAP) and Cro

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7 January 2021

Site-directed hydroxyl radical probing of the rRNA neighborhood of ribosomal protein S5.

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7 January 2021

Positioning of two alpha subunit carboxy-terminal domains of RNA polymerase at promoters by two transcription factors

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7 January 2021

Dimeric association of Escherichia coli RNA polymerase alpha subunits, studied by cleavage of single-cysteine alpha subunits conjugated to iron-(S)-1-[p-(bromoacetamido)benzyl]ethylenediaminetetraacetate

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7 January 2021

Mapping the sigma70 subunit contact sites on Escherichia coli RNA polymerase with a sigma70-conjugated chemical protease

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7 January 2021

Mapping protein-protein interactions with a library of tethered cutting reagents: the binding site of sigma 70 on Escherichia coli RNA polymerase

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7 January 2021

Proteolytic DNA for mapping protein-DNA interactions

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7 January 2021

Conjugation of antibodies with bifunctional chelating agents: isothiocyanate and bromoacetamide reagents, methods of analysis, and subsequent addition of metal ions

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7 January 2021

Electrophilic chelating agents for irreversible binding of metal chelates to engineered antibodies.

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7 January 2021

Protein-protein interactions mapped by artificial proteases: where sigma factors bind to RNA polymerase

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7 January 2021

Differential reactivity of maleimide and bromoacetyl functions with thiols: application to the preparation of liposomal diepitope constructs

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7 January 2021

57 Sequencing end-labeled DNA with base-specific chemical cleavages

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7 January 2021

Studies of the functional topography of Escherichia coli RNA polymerase. A method for localization of the sites of affinity labelling

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7 January 2021

Modular organization of the catalytic center of RNA polymerase

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7 January 2021

A microfluorometric assay for cholinesterases, suitable for multiple kinetic determinations of picomoles of released thiocholine.

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7 January 2021

Disulfide crosslinking of Escherichia coli ribosomal proteins with 2-iminothiolane (methyl 4-mercaptobutyrimidate): evidence that the crosslinked protein pairs are formed in the intact ribosomal subunit

1 reference

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7 January 2021

Simplified method for conjugating macrocyclic bifunctional chelating agents to antibodies via 2-iminothiolane.

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7 January 2021

Chemical conversion of a DNA-binding protein into a site-specific nuclease

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7 January 2021

Site-specific HU binding in the Mu transpososome: conversion of a sequence-independent DNA-binding protein into a chemical nuclease

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7 January 2021

A general method for highly selective cross-linking of unprotected polypeptides via pH-controlled modification of N-terminal alpha-amino groups

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7 January 2021

47 A centrifuged-column procedure for the measurement of ligand binding by beef heart F1

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7 January 2021

Novel combinatorial selection of phosphorothioate oligonucleotide aptamers

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7 January 2021

Antibodies against metal chelates

1 reference

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7 January 2021

Bipartite functional map of the E. coli RNA polymerase α subunit: Involvement of the C-terminal region in transcription activation by cAMP-CRP

1 reference

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7 January 2021

Determination of the promoter strength in the mixed transcription system. II. Promoters of ribosomal RNA, ribosomal protein S1 and recA protein operons from Escherichia coli

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2803%2971006-4

7 January 2021

Determination of the promoter strength in the mixed transcription system: promoters of lactose, tryplophan and ribosomal protein L10 operons fromExcherichia Coli

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2803%2971006-4

7 January 2021

Structural organization of the RNA polymerase-promoter open complex

1 reference

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7 January 2021

Binding of the sigma 70 protein to the core subunits of Escherichia coli RNA polymerase, studied by iron-EDTA protein footprinting

1 reference

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7 January 2021

Tissue sulfhydryl groups

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7 January 2021

Determination of free amino groups in proteins by trinitrobenzenesulfonic acid

1 reference

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7 January 2021

A fluorometric assay of the degree of modification of protein primary amines with polyethylene glycol

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7 January 2021

Cleavage at Asn-Gly bonds with hydroxylamine

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7 January 2021

Synthetic peptide vaccine design: synthesis and properties of a high-density multiple antigenic peptide system

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7 January 2021

Structural basis of transcription initiation: RNA polymerase holoenzyme at 4 A resolution

1 reference

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7 January 2021

Structural basis of transcription initiation: an RNA polymerase holoenzyme-DNA complex

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7 January 2021

Crystal structure of a bacterial RNA polymerase holoenzyme at 2.6 A resolution

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7 January 2021

The sigma 70 family: sequence conservation and evolutionary relationships

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2803%2971006-4

7 January 2021

Organization of open complexes at Escherichia coli promoters. Location of promoter DNA sites close to region 2.5 of the sigma70 subunit of RNA polymerase

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2803%2971006-4

7 January 2021

Mapping the promoter DNA sites proximal to conserved regions of sigma 70 in an Escherichia coli RNA polymerase-lacUV5 open promoter complex

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2803%2971006-4

7 January 2021

SWISS-MODEL and the Swiss-PdbViewer: an environment for comparative protein modeling

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2803%2971006-4

7 January 2021

10.1016/S0076-6879(03)71006-4

Identifiers

DOI

1 reference

5 December 2017

1 reference