(Q44731897)

5 December 2017

High level cell-free expression and specific labeling of integral membrane proteins (English)

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5 December 2017

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5

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5 December 2017

7

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5 December 2017

Frank Bernhard

8

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author name string

Christian Klammt

1

1 reference

5 December 2017

Frank Löhr

2

1 reference

5 December 2017

Birgit Schäfer

3

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5 December 2017

Winfried Haase

4

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5 December 2017

Heinz Rüterjans

6

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5 December 2017

language of work or name

English

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publication date

1 February 2004

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5 December 2017

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5 December 2017

271

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5 December 2017

568-580

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5 December 2017

3

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Biophysical approaches to membrane protein structure determination

5 December 2017

1 reference

https://api.crossref.org/works/10.1111%2FJ.1432-1033.2003.03959.X

Overexpression of mammalian integral membrane proteins for structural studies.

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1432-1033.2003.03959.X

Practical aspects of overexpressing bacterial secondary membrane transporters for structural studies.

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1432-1033.2003.03959.X

Gene expression in cell-free system on preparative scale

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1432-1033.2003.03959.X

Molecular properties of bacterial multidrug transporters

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1432-1033.2003.03959.X

Negative dominance studies demonstrate the oligomeric structure of EmrE, a multidrug antiporter from Escherichia coli.

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1432-1033.2003.03959.X

Overexpression of the Escherichia coli sugE gene confers resistance to a narrow range of quaternary ammonium compounds

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1432-1033.2003.03959.X

A new family of amino-acid-efflux proteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1432-1033.2003.03959.X

YfiK from Escherichia coli promotes export of O-acetylserine and cysteine

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1432-1033.2003.03959.X

In vitro synthesis of protein in microbial systems

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1432-1033.2003.03959.X

Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1432-1033.2003.03959.X

PHD: predicting one-dimensional protein structure by profile-based neural networks

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1432-1033.2003.03959.X

Attenuated T2 relaxation by mutual cancellation of dipole-dipole coupling and chemical shift anisotropy indicates an avenue to NMR structures of very large biological macromolecules in solution

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1432-1033.2003.03959.X

NMR investigation of the multidrug transporter EmrE, an integral membrane protein

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1432-1033.2003.03959.X

Optimization of expression and the purification by organic extraction of the integral membrane protein EmrE.

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1432-1033.2003.03959.X

A highly efficient and robust cell-free protein synthesis system prepared from wheat embryos: plants apparently contain a suicide system directed at ribosomes

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1432-1033.2003.03959.X

Determining the secondary structure and orientation of EmrE, a multi-drug transporter, indicates a transmembrane four-helix bundle.

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1432-1033.2003.03959.X

Effects of detergents on the secondary structures of prion protein peptides as studied by CD spectroscopy

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1432-1033.2003.03959.X

Examination of EmrE conformational differences in various membrane mimetic environments

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1432-1033.2003.03959.X

A membrane-embedded glutamate is required for ligand binding to the multidrug transporter EmrE

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1432-1033.2003.03959.X

In vitro translation and assembly of a complete T cell receptor-CD3 complex

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1432-1033.2003.03959.X

NMR analysis of in vitro-synthesized proteins without purification: a high-throughput approach.

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1432-1033.2003.03959.X

Cell-free synthesis and amino acid-selective stable isotope labeling of proteins for NMR analysis.

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1432-1033.2003.03959.X

Dual amino acid-selective and site-directed stable-isotope labeling of the human c-Ha-Ras protein by cell-free synthesis

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1432-1033.2003.03959.X

Protein expression systems for structural genomics and proteomics

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1432-1033.2003.03959.X

Cell-free production and stable-isotope labeling of milligram quantities of proteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1432-1033.2003.03959.X

Scanning Cysteine Accessibility of EmrE, an H+-coupled Multidrug Transporter fromEscherichia coli, Reveals a Hydrophobic Pathway for Solutes

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1432-1033.2003.03959.X

Rapid protein-folding assay using green fluorescent protein

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1432-1033.2003.03959.X

Green fluorescent protein as an indicator to monitor membrane protein overexpression in Escherichia coli

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1432-1033.2003.03959.X

31P-CP-MAS NMR studies on TPP+ bound to the ion-coupled multidrug transport protein EmrE

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1432-1033.2003.03959.X

10.1111/J.1432-1033.2003.03959.X

21 January 2018

Identifiers

DOI

1 reference

5 December 2017

1 reference