(Q46968954)

27 December 2017

title

Transient kinetics, fluorescence, and FRET in studies of initiation of translation in bacteria (English)

1 reference

27 December 2017

author

Pohl Milón

1

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27 December 2017

Andrey L. Konevega

2

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27 December 2017

Attilio Fabbretti

4

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27 December 2017

Marina Rodnina

6

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27 December 2017

author name string

Frank Peske

3

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27 December 2017

Claudio O Gualerzi

5

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27 December 2017

language of work or name

English

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publication date

1 January 2007

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27 December 2017

published in

Methods in Enzymology

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27 December 2017

volume

430

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27 December 2017

page(s)

1-30

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27 December 2017

The cryo-EM structure of a translation initiation complex from Escherichia coli

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X-ray crystallography shows that translational initiation factor IF3 consists of two compact alpha/beta domains linked by an alpha-helix

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The unusual translational initiation codon AUU limits the expression of the infC (initiation factor IF3) gene of Escherichia coli.

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Direct, real-time measurement of rapid inorganic phosphate release using a novel fluorescent probe and its application to actomyosin subfragment 1 ATPase

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Mechanism of translational initiation in prokaryotes

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7 January 2021

Crystal structure of an initiation factor bound to the 30S ribosomal subunit

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7 January 2021

Translation initiation factor IF2 interacts with the 30 S ribosomal subunit via two separate binding sites

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Interaction of Escherichia coli translation-initiation factor IF-1 with ribosomes.

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7 January 2021

Interaction of translation initiation factor IF1 with the E. coli ribosomal A site.

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Interaction of translation initiation factor 3 with the 30S ribosomal subunit

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7 January 2021

Translational standby sites: how ribosomes may deal with the rapid folding kinetics of mRNA.

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7 January 2021

Ribosomal subunit interaction as studied by light scattering. Evidence of different classes of ribosome preparations.

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7 January 2021

The real-time path of translation factor IF3 onto and off the ribosome.

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7 January 2021

Preferential translation of cold-shock mRNAs during cold adaptation

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7 January 2021

Kinetic determinants of high-fidelity tRNA discrimination on the ribosome

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7 January 2021

Kinetic mechanism of elongation factor Ts-catalyzed nucleotide exchange in elongation factor Tu.

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7 January 2021

Light-scattering studies showing the effect of initiation factors on the reversible dissociation of Escherichia coli ribosomes

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7 January 2021

Initial rate kinetic analysis of the mechanism of initiation complex formation and the role of initiation factor IF-3

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7 January 2021

Initiation factors in the early events of mRNA translation in bacteria

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7 January 2021

Initiation of mRNA translation in prokaryotes

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7 January 2021

A time-resolved investigation of ribosomal subunit association.

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7 January 2021

Translational Operator of mRNA on the Ribosome: How Repressor Proteins Exclude Ribosome Binding

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7 January 2021

Distance moved by transfer RNA during translocation from the A site to the P site on the ribosome

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7 January 2021

Novel roles for classical factors at the interface between translation termination and initiation

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7 January 2021

The importance of structural transitions of the switch II region for the functions of elongation factor Tu on the ribosome

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7 January 2021

Single-step purification of specific tRNAs by hydrophobic tagging.

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7 January 2021

Prokaryotic translation initiation factor IF3 is an elongated protein consisting of two crystallizable domains.

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7 January 2021

Translation of mRNAs with degenerate initiation triplet AUU displays high initiation factor 2 dependence and is subject to initiation factor 3 repression

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7 January 2021

The NH2-terminal cleavage of Escherichia coli translational initiation factor IF3. A mechanism to control the intracellular level of the factor?

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7 January 2021

Initiation of protein synthesis in bacteria

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7 January 2021

Ribosomal localization of translation initiation factor IF2

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7 January 2021

Location of translational initiation factor IF3 on the small ribosomal subunit

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7 January 2021

The nucleotide-binding site of bacterial translation initiation factor 2 (IF2) as a metabolic sensor

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7 January 2021

Specific protection of 16 S rRNA by translational initiation factors

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7 January 2021

Conformational transition of initiation factor 2 from the GTP- to GDP-bound state visualized on the ribosome.

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7 January 2021

Hydrogen exchange studies on ribosomes.

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7 January 2021

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7 January 2021

Mapping the active sites of bacterial translation initiation factor IF3.

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7 January 2021

Translation initiation factor IF3: two domains, five functions, one mechanism?

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7 January 2021

Crystal structures of complexes of the small ribosomal subunit with tetracycline, edeine and IF3.

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7 January 2021

Kinetic Analysis of Interaction of Eukaryotic Release Factor 3 with Guanine Nucleotides

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7 January 2021

Effect of Initiation Factor 3 Binding on the 30S Ribosomal Subunits of Escherichia coli

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7 January 2021

18 Qualitative and semiquantitative assay of Escherichia coli translational initiation factor IF-3

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7 January 2021

Mechanism of protein biosynthesis in prokaryotic cells. Effect of initiation factor IF1 on the initial rate of 30 S initiation complex formation

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7 January 2021

Mechanism of translational initiation in prokaryotes. IF3 is released from ribosomes during and not before 70 S initiation complex formation.

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7 January 2021

Improved plasmid vectors with a thermoinducible expression and temperature-regulated runaway replication

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7 January 2021

Specificity and Properties of the Destabilization, Induced by Initiation Factor IF-3, of Ternary Complexes of the 30-S Ribosomal Subunit, Aminoacyl-tRNA and Polynucleotides

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2930001-3

7 January 2021

Transient conformational states of aminoacyl-tRNA during ribosome binding catalyzed by elongation factor Tu.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2930001-3

7 January 2021

Thiostrepton inhibits the turnover but not the GTPase of elongation factor G on the ribosome.

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7 January 2021

Purification of fMet-tRNA(fMet) by fast protein liquid chromatography.

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https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2930001-3

7 January 2021

GTPases mechanisms and functions of translation factors on the ribosome

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https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2930001-3

7 January 2021

X-Ray structures of the universal translation initiation factor IF2/eIF5B: conformational changes on GDP and GTP binding

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https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2930001-3

7 January 2021

An elongation factor G-induced ribosome rearrangement precedes tRNA-mRNA translocation

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2930001-3

7 January 2021

The structure of the translational initiation factor IF1 from E.coli contains an oligomer-binding motif

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https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2930001-3

7 January 2021

Unfolding of mRNA secondary structure by the bacterial translation initiation complex

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https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2930001-3

7 January 2021

Late events of translation initiation in bacteria: a kinetic analysis

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https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2930001-3

7 January 2021

Role and timing of GTP binding and hydrolysis during EF-G-dependent tRNA translocation on the ribosome

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7 January 2021

Effect of Escherichia coli initiation factors on the kinetics of N-AcPhe-tRNAPhe binding to 30S ribosomal subunits. A fluorescence stopped-flow study

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7 January 2021

53 Replacement of odd bases in tRNA by fluorescent dyes

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7 January 2021

Kinetics of the reversible association of ribosomal subunits: Stopped-flow studies of the rate law and of the effect of Mg2+

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7 January 2021

Structural basis for messenger RNA movement on the ribosome

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7 January 2021

The path of messenger RNA through the ribosome

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https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2930001-3

7 January 2021

Magnesium dependence and equilibrium of the Escherichia coli ribosomal subunit association

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https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2930001-3

7 January 2021

10.1016/S0076-6879(07)30001-3

Identifiers

DOI

1 reference

27 December 2017

1 reference

27 December 2017