(Q46970709)

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:16510443%20AND%20SRC:MED&resulttype=core&format=json

17 January 2020

title

Evolutional design of a hyperactive cysteine- and methionine-free mutant of Escherichia coli dihydrofolate reductase (English)

1 reference

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17 January 2020

1 reference

4

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17 January 2020

Akiko Yokota

5

1 reference

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17 January 2020

author name string

Masahiro Iwakura

1

1 reference

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17 January 2020

Kosuke Maki

2

1 reference

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17 January 2020

Hisashi Takahashi

3

1 reference

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17 January 2020

Katsuo Katayanagi

6

1 reference

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17 January 2020

Tadashi Kamiyama

7

1 reference

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17 January 2020

Kunihiko Gekko

8

1 reference

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17 January 2020

language of work or name

English

0 references

publication date

1 March 2006

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:16510443%20AND%20SRC:MED&resulttype=core&format=json

Journal of Biological Chemistry

17 January 2020

number of pages

13

1 reference

based on heuristic

inferred from page(s)

published in

1 reference

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17 January 2020

volume

281

1 reference

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17 January 2020

page(s)

13234-13246

1 reference

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17 January 2020

issue

19

1 reference

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Principles that govern the folding of protein chains

17 January 2020

cites work

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M508823200

Applications of DNA shuffling to pharmaceuticals and vaccines

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M508823200

Rapid evolution of a protein in vitro by DNA shuffling

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M508823200

Tuning the activity of an enzyme for unusual environments: sequential random mutagenesis of subtilisin E for catalysis in dimethylformamide

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M508823200

Directed evolution of an aspartate aminotransferase with new substrate specificities

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M508823200

Enhanced degradation of polychlorinated biphenyls by directed evolution of biphenyl dioxygenase.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M508823200

Dihydrofolate Reductase Gene as a Versatile Expression Marker

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M508823200

Construction and evaluation of the kinetic scheme associated with dihydrofolate reductase from Escherichia coli

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M508823200

Crystal structures of Escherichia coli dihydrofolate reductase: the NADP+ holoenzyme and the folate.NADP+ ternary complex. Substrate binding and a model for the transition state

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M508823200

Crystal structure of unliganded Escherichia coli dihydrofolate reductase. Ligand-induced conformational changes and cooperativity in binding

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M508823200

Isomorphous crystal structures of Escherichia coli dihydrofolate reductase complexed with folate, 5-deazafolate, and 5,10-dideazatetrahydrofolate: mechanistic implications

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M508823200

Loop and subdomain movements in the mechanism of Escherichia coli dihydrofolate reductase: crystallographic evidence

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M508823200

Probing the functional role of phenylalanine-31 of Escherichia coli dihydrofolate reductase by site-directed mutagenesis.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M508823200

Hydrophobic interactions via mutants of Escherichia coli dihydrofolate reductase: separation of binding and catalysis.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M508823200

The function of amino acid residues contacting the nicotinamide ring of NADPH in dihydrofolate reductase from Escherichia coli

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M508823200

Nonadditivity of mutational effects at the folate binding site of Escherichia coli dihydrofolate reductase

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M508823200

Molecular basis for nonadditive mutational effects in Escherichia coli dihydrofolate reductase

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M508823200

Additivity of mutational effects in proteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M508823200

Long-range electrostatic interactions can influence the folding, stability, and cooperativity of dihydrofolate reductase.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M508823200

A strategy for testing the suitability of cysteine replacements in dihydrofolate reductase from Escherichia coli.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M508823200

A reexamination of the folding mechanism of dihydrofolate reductase from Escherichia coli: verification and refinement of a four-channel model.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M508823200

Folding of dihydrofolate reductase from Escherichia coli.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M508823200

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M508823200

Mechanism of the reaction catalyzed by dihydrofolate reductase from Escherichia coli: pH and deuterium isotope effects with NADPH as the variable substrate

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M508823200

Single amino acid substitutions in flexible loops can induce large compressibility changes in dihydrofolate reductase.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M508823200

Unfolding free energy changes determined by the linear extrapolation method. 1. Unfolding of phenylmethanesulfonyl alpha-chymotrypsin using different denaturants

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M508823200

Cyanocysteine-mediated molecular dissection of dihydrofolate reductase: occurrence of intra- and inter-molecular reactions forming a peptide bond.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M508823200

Ribozymes: aiming at RNA replication and protein synthesis

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M508823200

Landscapes: complex optimization problems and biopolymer structures

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M508823200

Generation and use of synthetic peptide combinatorial libraries for basic research and drug discovery

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M508823200

Extent of N-terminal methionine excision from Escherichia coli proteins is governed by the side-chain length of the penultimate amino acid

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M508823200

Thermodynamic fluctuations in protein molecules

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M508823200

Compressibility-structure relationship of globular proteins.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M508823200

Kinetics of substrate, coenzyme, and inhibitor binding to Escherichia coli dihydrofolate reductase.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M508823200

Transient intermediates in the folding of dihydrofolate reductase as detected by far-ultraviolet circular dichroism spectroscopy.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M508823200

A cross-section of the fitness landscape of dihydrofolate reductase

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M508823200

Surveying a local fitness landscape of a protein with epistatic sites for the study of directed evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M508823200

Phosphatidylcholine activation of human heart (R)-3-hydroxybutyrate dehydrogenase mutants lacking active center sulfhydryls: site-directed mutagenesis of a new recombinant fusion protein

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M508823200

Enhancing oxidative resistance of Agrobacterium radiobacter N-carbamoyl D-amino acid amidohydrolase by engineering solvent-accessible methionine residues

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M508823200

Comparing the effect on protein stability of methionine oxidation versus mutagenesis: steps toward engineering oxidative resistance in proteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M508823200

Probing minimal independent folding units in dihydrofolate reductase by molecular dissection

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M508823200

An approach for protein to be completely reversible to thermal denaturation even at autoclave temperatures

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M508823200

Protein folding and association: insights from the interfacial and thermodynamic properties of hydrocarbons

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M508823200

Stretching exercises--flexibility in dihydrofolate reductase catalysis

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M508823200

Testing the relationship between foldability and the early folding events of dihydrofolate reductase from Escherichia coli

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M508823200

Effects of the difference in the unfolded-state ensemble on the folding of Escherichia coli dihydrofolate reductase

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M508823200

Probing the interactions between the folding elements early in the folding of Escherichia coli dihydrofolate reductase by systematic sequence perturbation analysis

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M508823200

Calculation of molecular volumes and areas for structures of known geometry.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M508823200

MOLSCRIPT: a program to produce both detailed and schematic plots of protein structures

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M508823200