(Q47613006)

4 February 2018

title

Engineering stable cytoplasmic intrabodies with designed specificity (English)

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4 February 2018

6

Anna Tramontano

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4 February 2018

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Marcello Donini

1

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4 February 2018

Veronica Morea

2

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4 February 2018

Angiola Desiderio

3

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4 February 2018

Dimitre Pashkoulov

4

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4 February 2018

Maria Elena Villani

5

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4 February 2018

Eugenio Benvenuto

7

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4 February 2018

language of work or name

English

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publication date

1 July 2003

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4 February 2018

Journal of Molecular Biology

published in

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4 February 2018

volume

330

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4 February 2018

issue

2

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4 February 2018

page(s)

323-332

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4 February 2018

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900530-8

1 reference

Single-chain antigen-binding proteins

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Intracellular immunization with cytosolic recombinant antibodies.

7 January 2021

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Transgenic plants expressing a functional single-chain Fv antibody are specifically protected from virus attack

7 January 2021

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Synthesis of a functional anti-phytochrome single-chain Fv protein in transgenic tobacco

7 January 2021

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Potent inhibition of human immunodeficiency virus type 1 replication by an intracellular anti-Rev single-chain antibody

7 January 2021

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Effects of intracellular expression of anti-huntingtin antibodies of various specificities on mutant huntingtin aggregation and toxicity

7 January 2021

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Functional neutralization of HIV-1 Vif protein by intracellular immunization inhibits reverse transcription and viral replication

7 January 2021

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Expression of a single-chain Fv antibody against abscisic acid creates a wilty phenotype in transgenic tobacco

7 January 2021

1 reference

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Diverting a protein from its cellular location by intracellular antibodies. The case of p21Ras

7 January 2021

1 reference

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Immunomodulation of enzyme function in plants by single-domain antibody fragments

7 January 2021

1 reference

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Stability engineering of antibody single-chain Fv fragments

7 January 2021

1 reference

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Different Equilibrium Stability Behavior of ScFv Fragments: Identification, Classification, and Improvement by Protein Engineering†

7 January 2021

1 reference

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Antibody scFv fragments without disulfide bonds made by molecular evolution

7 January 2021

1 reference

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Expression of an antibody fragment at high levels in the bacterial cytoplasm

7 January 2021

1 reference

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Intracellular antibody capture technology: application to selection of intracellular antibodies recognising the BCR-ABL oncogenic protein

7 January 2021

1 reference

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Selection of antibodies for intracellular function using a two-hybrid in vivo system

7 January 2021

1 reference

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In vitro folding and thermodynamic stability of an antibody fragment selected in vivo for high expression levels in Escherichia coli cytoplasm

7 January 2021

1 reference

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Sequence statistics reliably predict stabilizing mutations in a protein domain

7 January 2021

1 reference

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Engineered turns of a recombinant antibody improve its in vivo folding

7 January 2021

1 reference

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Selection for improved protein stability by phage display.

7 January 2021

1 reference

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Disrupting the hydrophobic patches at the antibody variable/constant domain interface: improved in vivo folding and physical characterization of an engineered scFv fragment.

7 January 2021

1 reference

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Improving in vivo folding and stability of a single-chain Fv antibody fragment by loop grafting

7 January 2021

1 reference

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A natural antibody missing a cysteine in VH: consequences for thermodynamic stability and folding.

7 January 2021

1 reference

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Correlation betweenin VitroStability andin VivoPerformance of Anti-GCN4 Intrabodies as Cytoplasmic Inhibitors

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900530-8

A single-chain antibody fragment is functionally expressed in the cytoplasm of both Escherichia coli and transgenic plants

7 January 2021

1 reference

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A semi-synthetic repertoire of intrinsically stable antibody fragments derived from a single-framework scaffold

7 January 2021

1 reference

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Bound water molecules and conformational stabilization help mediate an antigen-antibody association

7 January 2021

1 reference

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7 January 2021

1 reference

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Reshaping human antibodies for therapy

7 January 2021

1 reference

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Humanization of a mouse monoclonal antibody that blocks the epidermal growth factor receptor: recovery of antagonistic activity

7 January 2021

1 reference

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Canonical structures for the hypervariable regions of immunoglobulins

7 January 2021

1 reference

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7 January 2021

The Protein Data Bank

1 reference

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Antibody framework residues affecting the conformation of the hypervariable loops

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900530-8

Antibody-antigen interactions: contact analysis and binding site topography.

7 January 2021

1 reference

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Standard conformations for the canonical structures of immunoglobulins

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900530-8

Framework residue 71 is a major determinant of the position and conformation of the second hypervariable region in the VH domains of immunoglobulins

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900530-8

Conformations of immunoglobulin hypervariable regions

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900530-8

Conformations of the third hypervariable region in the VH domain of immunoglobulins

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900530-8

Structural classification of CDR-H3 in antibodies

7 January 2021

1 reference

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H3-rules: identification of CDR-H3 structures in antibodies

7 January 2021

1 reference

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Antibody modeling: implications for engineering and design.

7 January 2021

1 reference

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Radioactive labeling of recombinant antibody fragments by phosphorylation using human casein kinase II and [gamma-32P]-ATP.

7 January 2021

1 reference

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Characterization of the Erwinia carotovora pelB gene and its product pectate lyase

7 January 2021

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A general method for maximizing the expression of a cloned gene

7 January 2021

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Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins

7 January 2021

1 reference

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Calculation of protein extinction coefficients from amino acid sequence data

7 January 2021

1 reference

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A mutational analysis of the binding of two different proteins to the same antibody

7 January 2021

1 reference

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10.1016/S0022-2836(03)00530-8

7 January 2021

Identifiers

DOI

1 reference

4 February 2018

1 reference

4 February 2018