(Q47948648)

7 February 2018

title

Engineered Fv fragments as a tool for the one-step purification of integral multisubunit membrane protein complexes (English)

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7 February 2018

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4

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7 February 2018

Hartmut Michel

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object named as

Michel H

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7 February 2018

author name string

Kleymann G

1

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7 February 2018

Ostermeier C

2

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7 February 2018

Ludwig B

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7 February 2018

publication date

1 February 1995

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7 February 2018

published in

Bio/technology

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7 February 2018

volume

13

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7 February 2018

issue

2

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7 February 2018

page(s)

155-160

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7 February 2018

Crystal structure of a streptococcal protein G domain bound to an Fab fragment

cites work

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-155

Bacterial expression of immunoglobulin fragments

21 January 2018

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https://api.crossref.org/works/10.1038%2FNBT0295-155

An Hsp70-like protein in the ER: identity with the 78 kd glucose-regulated protein and immunoglobulin heavy chain binding protein

21 January 2018

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https://api.crossref.org/works/10.1038%2FNBT0295-155

A general vector, pASK84, for cloning, bacterial production, and single-step purification of antibody Fab fragments

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-155

A general method for chimerization of monoclonal antibodies by inverse polymerase chain reaction which conserves authentic N-terminal sequences

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-155

Making antibody fragments using phage display libraries

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-155

One-step affinity purification of bacterially produced proteins by means of the "Strep tag" and immobilized recombinant core streptavidin.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-155

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-155

Overproduction of membrane proteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-155

Comparison of energy-transducing capabilities of the two- and three-subunit cytochromes aa3 from Paracoccus denitrificans and the 13-subunit beef heart enzyme

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-155

7 January 2021

Affinity chromatography purification of cytochrome c oxidase and b-c1 complex from beef heart mitochondria. Use of thiol-sepharose-bound Saccharomyces cerevisiae cytochrome c

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-155

7 January 2021

Engineering proteins to facilitate bioprocessing

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-155

7 January 2021

Selective enzyme purification by affinity chromatography

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-155

7 January 2021

Continuous cultures of fused cells secreting antibody of predefined specificity

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-155

7 January 2021

A monoclonal antibody for large-scale purification of human leukocyte interferon

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https://api.crossref.org/works/10.1038%2FNBT0295-155

7 January 2021

Immunologic Adsorbents: I. Isolation of Antibody by Means of a Cellulose-Protein Antigen

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https://api.crossref.org/works/10.1038%2FNBT0295-155

7 January 2021

Use of Antibodies on an Insoluble Support for Specific Detection of Radioactive Antigens

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-155

7 January 2021

Immobilization of Fv antibody fragments on porous silica and their utility in affinity chromatography

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-155

7 January 2021

Immunoaffinity chromatography of proteins

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-155

7 January 2021

A support for affinity chromatography that covalently binds amino groups via a cleavable connector arm

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-155

7 January 2021

Immunoaffinity isolation of membrane antigens with biotinylated monoclonal antibodies and streptavidin-agarose

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-155

7 January 2021

Assembly of a functional immunoglobulin Fv fragment in Escherichia coli

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https://api.crossref.org/works/10.1038%2FNBT0295-155

7 January 2021

The random peptide library-assisted engineering of a C-terminal affinity peptide, useful for the detection and purification of a functional Ig Fv fragment.

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-155

7 January 2021

Cloning immunoglobulin variable domains for expression by the polymerase chain reaction

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-155

7 January 2021

A novel and rapid cloning method for the T-cell receptor variable region sequences

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-155

7 January 2021

Complete variable region sequence of a nonfunctionally rearranged kappa light chain transcribed in the nonsecretor P3-X63-Ag8.653 myeloma cell line

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-155

7 January 2021

Calculation of protein extinction coefficients from amino acid sequence data

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-155

7 January 2021

A two-subunit cytochrome c oxidase (cytochrome aa3) from Paracoccus dentrificans

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-155

7 January 2021

Blue native electrophoresis for isolation of membrane protein complexes in enzymatically active form

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-155

7 January 2021

Monoclonal antibodies to sucrase/isomaltase: probes for the study of postnatal development and biogenesis of the intestinal microvillus membrane

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-155

7 January 2021

DNA sequencing with chain-terminating inhibitors

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-155

7 January 2021

Paracoccus denitrificans cytochrome c1 gene replacement mutants

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-155

7 January 2021

Peptide and protein molecular weight determination by electrophoresis using a high-molarity tris buffer system without urea

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-155

7 January 2021

Identifiers

DOI

10.1038/NBT0295-155

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7 February 2018

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7 February 2018