(Q50537711)

15 March 2018

title

Generation of stable, high-producing CHO cell lines by lentiviral vector-mediated gene transfer in serum-free suspension culture. (English)

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15 March 2018

author name string

Agata Oberbek

1

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Mattia Matasci

2

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David L Hacker

3

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Florian M Wurm

4

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publication date

23 November 2010

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Biotechnology and Bioengineering

published in

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volume

108

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issue

3

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page(s)

600-610

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Dose-dependent transduction of vesicular stomatitis virus G protein-pseudotyped retrovirus vector into human solid tumor cell lines and murine fibroblasts

cites work

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Efficient control of gene expression in the hematopoietic system using a single Tet-on inducible lentiviral vector

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Characterization of the stability of recombinant protein production in the GS-NS0 expression system

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Selection methods for high-producing mammalian cell lines.

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Genome-wide analysis of retroviral DNA integration

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Calcium phosphate transfection generates mammalian recombinant cell lines with higher specific productivity than polyfection

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Accelerated clone selection for recombinant CHO CELLS using a FACS-based high-throughput screen

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Site-specific gene targeting for gene expression in eukaryotes

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Autoregulated, bidirectional and multicistronic gas-inducible mammalian as well as lentiviral expression vectors

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Transfection of adherent and suspended cells by calcium phosphate

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Homogeneity and persistence of transgene expression by omitting antibiotic selection in cell line isolation

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Production, concentration and titration of pseudotyped HIV-1-based lentiviral vectors

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Employing epigenetics to augment the expression of therapeutic proteins in mammalian cells

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Optimization of protein production in mammalian cells with a coexpressed fluorescent marker

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Transient gene expression: recombinant protein production with suspension-adapted HEK293-EBNA cells

21 January 2018

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Design and in vivo characterization of self-inactivating human and non-human lentiviral expression vectors engineered for streptogramin-adjustable transgene expression

21 January 2018

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Orbital shaker technology for the cultivation of mammalian cells in suspension.

21 January 2018

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Scalable transient gene expression in Chinese hamster ovary cells in instrumented and non-instrumented cultivation systems

21 January 2018

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Efficient transfer, integration, and sustained long-term expression of the transgene in adult rat brains injected with a lentiviral vector

21 January 2018

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Lentiviral vectors interfering with virus-induced CD4 down-modulation potently block human immunodeficiency virus type 1 replication in primary lymphocytes

21 January 2018

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Accelerated cell line development using two-color fluorescence activated cell sorting to select highly expressing antibody-producing clones

21 January 2018

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New disposable tubes for rapid and precise biomass assessment for suspension cultures of mammalian cells

21 January 2018

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Lentiviral vectors: turning a deadly foe into a therapeutic agent

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A novel vector platform for vitamin H-inducible transgene expression in mammalian cells

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Harnessing HIV for therapy, basic research and biotechnology

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Rev-dependent lentiviral expression vector

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Production of recombinant protein therapeutics in cultivated mammalian cells

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Multiply attenuated lentiviral vector achieves efficient gene delivery in vivo

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Identifiers

DOI

10.1002/BIT.22968

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15 March 2018

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15 March 2018