(Q50905525)

25 March 2018

title

Improved production of membrane proteins in Escherichia coli by selective codon substitutions. (English)

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5

Gunnar von Heijne

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Morten H H Nørholm

1

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Stephen Toddo

2

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Minttu T I Virkki

3

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Sara Light

4

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Daniel O Daley

6

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language of work or name

English

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publication date

12 June 2013

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25 March 2018

published in

FEBS Letters

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volume

587

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issue

15

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page(s)

2352-2358

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25 March 2018

Overexpression of mammalian integral membrane proteins for structural studies.

cites work

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Overcoming the challenges of membrane protein crystallography.

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Codon bias and heterologous protein expression

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Coding-sequence determinants of gene expression in Escherichia coli

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Engineering genes for predictable protein expression

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You're one in a googol: optimizing genes for protein expression.

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Global topology analysis of the Escherichia coli inner membrane proteome

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A global topology map of the Saccharomyces cerevisiae membrane proteome

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Experimentally based topology models for E. coli inner membrane proteins

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A mutant Pfu DNA polymerase designed for advanced uracil-excision DNA engineering

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Optimization of membrane protein overexpression and purification using GFP fusions.

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Transient ribosomal attenuation coordinates protein synthesis and co-translational folding.

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Codon bias at the 3'-side of the initiation codon is correlated with translation initiation efficiency in Escherichia coli

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Protein disorder prediction: implications for structural proteomics

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Structure-based analysis of GPCR function: conformational adaptation of both agonist and receptor upon leukotriene B4 binding to recombinant BLT1.

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Gene optimization mechanisms: a multi-gene study reveals a high success rate of full-length human proteins expressed in Escherichia coli

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Codon optimization of Caenorhabditis elegans GluCl ion channel genes for mammalian cells dramatically improves expression levels.

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Optimal encoding rules for synthetic genes: the need for a community effort

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The anti-Shine-Dalgarno sequence drives translational pausing and codon choice in bacteria

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Initiation of protein synthesis in bacteria

21 January 2018

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Quantitative correlation between mRNA secondary structure around the region downstream of the initiation codon and translational efficiency in Escherichia coli.

21 January 2018

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Synonymous but not the same: the causes and consequences of codon bias

21 January 2018

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General nature of the genetic code for proteins

21 January 2018

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10.1016/J.FEBSLET.2013.05.063

21 January 2018

Identifiers

DOI

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25 March 2018

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25 March 2018