(Q54490146)

30 May 2018

title

Efficient production of isotopically labeled proteins by cell-free synthesis: a practical protocol. (English)

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30 May 2018

author

Masato Taoka

3

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author name string

Takuya Torizawa

1

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30 May 2018

Masato Shimizu

2

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30 May 2018

Hiroshi Miyano

4

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Masatsune Kainosho

5

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30 May 2018

publication date

1 November 2004

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30 May 2018

Journal of Biomolecular NMR

published in

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30 May 2018

volume

30

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30 May 2018

issue

3

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30 May 2018

page(s)

311-325

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30 May 2018

https://scigraph.springernature.com/pub.10.1007/s10858-004-3534-2

exact match

0 references

cites work

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ompT encodes the Escherichia coli outer membrane protease that cleaves T7 RNA polymerase during purification

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A novel approach for sequential assignment of 1H, 13C, and 15N spectra of proteins: heteronuclear triple-resonance three-dimensional NMR spectroscopy. Application to calmodulin

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Cell-free translation reconstituted with purified components

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An increased content of protease La, the lon gene product, increases protein degradation and blocks growth in Escherichia coli

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12 December 2020

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Rapid and simple purification of T7 RNA polymerase

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Backbone 1H, 13C, and 15N resonance assignments of an 18.2 kDa protein, E. coli peptidyl-prolyl cis-trans isomerase b (EPPIb)

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Degradation in vitro of bacteriophage lambda N protein by Lon protease from Escherichia coli

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Inclusion body formation and protein stability in sequence variants of interleukin-1 beta.

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Heteronuclear 3D NMR and isotopic labeling of calmodulin. Towards the complete assignment of the 1H NMR spectrum

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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

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High level cell-free expression and specific labeling of integral membrane proteins

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Lysis of Escherichia coli by induction of cloned ϕX174 genes

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Cell-free synthesis and amino acid-selective stable isotope labeling of proteins for NMR analysis.

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A highly efficient and robust cell-free protein synthesis system prepared from wheat embryos: plants apparently contain a suicide system directed at ribosomes

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12 December 2020

10.1007/S10858-004-3534-2

Identifiers

DOI

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30 May 2018

release_ldwsxg2jdna6bjoa73mded6hba

Fatcat ID

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Fatcat

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24 November 2022

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30 May 2018