(Q54635486)

1 June 2018

title

Bias and artifacts in multitemplate polymerase chain reactions (PCR). (English)

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1 June 2018

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Takahiro Kanagawa

1

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1 June 2018

publication date

1 January 2003

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1 June 2018

Journal of Bioscience and Bioengineering

published in

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1 June 2018

volume

96

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1 June 2018

page(s)

317-323

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1 June 2018

issue

4

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1 June 2018

Bias caused by template annealing in the amplification of mixtures of 16S rRNA genes by PCR

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Microvariation artifacts introduced by PCR and cloning of closely related 16S rRNA gene sequences

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An evaluation of terminal-restriction fragment length polymorphism (T-RFLP) analysis for the study of microbial community structure and dynamics

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Evaluation of PCR amplification bias by terminal restriction fragment length polymorphism analysis of small-subunit rRNA and mcrA genes by using defined template mixtures of methanogenic pure cultures and soil DNA extracts

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Optimization of annealing temperature to reduce bias caused by a primer mismatch in multitemplate PCR

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Methyl-directed repair of frameshift heteroduplexes in cell extracts from Escherichia coli

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Molecular matchmakers

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Fluorescent quenching-based quantitative detection of specific DNA/RNA using a BODIPY((R)) FL-labeled probe or primer

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Fluorescence-quenching phenomenon by photoinduced electron transfer between a fluorescent dye and a nucleotide base.

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7 January 2021

10.1016/S1389-1723(03)90130-7

Identifiers

DOI

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1 June 2018

PubMed ID

16233530

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1 June 2018