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Two-dimensional gel electrophoresis: an overview
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scholarly article
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title
Two-dimensional gel electrophoresis: an overview
(English)
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author
David E. Garfin
series ordinal
1
object named as
David E. Garfin
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publication date
May 2003
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published in
Trends in Analytical Chemistry
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volume
22
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issue
5
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page(s)
263-272
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cites work
Back to the future: the human protein index (HPI) and the agenda for post-proteomic biology
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Progress with gene-product mapping of the Mollicutes: Mycoplasma genitalium.
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Proteomics: quantitative and physical mapping of cellular proteins
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Two-dimensional gel electrophoresis in proteomics: old, old fashioned, but it still climbs up the mountains
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Two-dimensional electrophoresis of proteins: an updated protocol and implications for a functional analysis of the genome.
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Solubilization of proteins for electrophoretic analyses
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New zwitterionic detergents improve the analysis of membrane proteins by two-dimensional electrophoresis
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Effect of strong detergents and chaotropes on the detection of proteins in two-dimensional gels
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Extraction of membrane proteins by differential solubilization for separation using two-dimensional gel electrophoresis
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Proteomic analysis of the Escherichia coli outer membrane.
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A comprehensive two-dimensional map of cytosolic proteins of Bacillus subtilis
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Protein delipidation and precipitation by tri-n-butylphosphate, acetone, and methanol treatment for isoelectric focusing and two-dimensional gel electrophoresis
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Identification and quantitation of cysteine in proteins separated by gel electrophoresis.
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Reduction and alkylation of proteins in preparation of two-dimensional map analysis: why, when, and how?
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Chromatographic separations as a prelude to two-dimensional electrophoresis in proteomics analysis
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A turning point in proteome analysis: sample prefractionation via multicompartment electrolyzers with isoelectric membranes
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A method for global analysis of complex proteomes using sample prefractionation by solution isoelectrofocusing prior to two-dimensional electrophoresis
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New separation tools for comprehensive studies of protein expression by mass spectrometry
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Zooming-in on the proteome: very narrow-range immobilised pH gradients reveal more protein species and isoforms
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Detecting proteins separated by 2-D gel electrophoresis
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A modified silver staining protocol for visualization of proteins compatible with matrix-assisted laser desorption/ionization and electrospray ionization-mass spectrometry
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Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250.
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Environmentally benign staining procedure for electrophoresis gels using Coomassie Brilliant Blue.
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Digital imaging considerations for gel electrophoresis analysis systems
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Mass spectrometry and the age of the proteome
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Difference gel electrophoresis: a single gel method for detecting changes in protein extracts
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Quantitative protein profiling using two-dimensional gel electrophoresis, isotope-coded affinity tag labeling, and mass spectrometry
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Rainbow's end: the quest for multiplexed fluorescence quantitative analysis in proteomics
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Two-dimensional gel electrophoresis; better than a poke in the ICAT?
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Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa
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Profiling the alkaline membrane proteome of Caulobacter crescentus with two-dimensional electrophoresis and mass spectrometry
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Whole proteome pI values correlate with subcellular localizations of proteins for organisms within the three domains of life.
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DOI
10.1016/S0165-9936(03)00506-5
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