(Q60305396)

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Methods of single-molecule fluorescence spectroscopy and microscopy

article by W. E. Moerner & David P. Fromm published August 2003 in Review of Scientific Instruments

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Methods of single-molecule fluorescence spectroscopy and microscopy (English)

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main subject

fluorescence spectroscopy

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1

W. E. Moerner

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author name string

David P. Fromm

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2

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publication date

August 2003

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published in

Review of Scientific Instruments

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volume

74

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issue

8

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page(s)

3597-3619

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cites work

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1 reference

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1 reference

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1 reference

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1 reference

11 October 2022

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1 reference

11 October 2022

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1 reference

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1 reference

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1 reference

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1 reference

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1 reference

11 October 2022

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1 reference

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1 reference

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1 reference

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1 reference

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1 reference

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1 reference

11 October 2022

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1 reference

11 October 2022

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1 reference

11 October 2022

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Scanning near-field optical microscopy with aperture probes: Fundamentals and applications

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1 reference

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1 reference

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10.1063/1.1589587

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(Q60305396)

Methods of single-molecule fluorescence spectroscopy and microscopy

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