(Q80407570)

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:15289056%20AND%20SRC:MED&resulttype=core&format=json

31 December 2019

title

Gene site saturation mutagenesis: a comprehensive mutagenesis approach (English)

1 reference

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31 December 2019

author name string

Keith A Kretz

1

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31 December 2019

Toby H Richardson

2

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31 December 2019

Kevin A Gray

3

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31 December 2019

Dan E Robertson

4

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31 December 2019

Xuqiu Tan

5

1 reference

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31 December 2019

Jay M Short

6

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31 December 2019

publication date

1 January 2004

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31 December 2019

published in

Methods in Enzymology

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31 December 2019

volume

388

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31 December 2019

page(s)

3-11

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Modifications of theE.coliLac repressor for expression in eukaryotic cells: effects of nuclear signal sequences on protein activity and nuclear accumulation

31 December 2019

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Improving the quality of industrially important enzymes by directed evolution

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Metabolic engineering and directed evolution for the production of pharmaceuticals

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Conversion from farnesyl diphosphate synthase to geranylgeranyl diphosphate synthase by random chemical mutagenesis.

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A general method for saturation mutagenesis of cloned DNA fragments

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Site-directed mutagenesis by overlap extension using the polymerase chain reaction

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PCR-mediated recombination and mutagenesis. SOEing together tailor-made genes

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Systematic mutation of bacteriophage T4 lysozyme.

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Complete cysteine-scanning mutagenesis and site-directed chemical modification of the Tn10-encoded metal-tetracycline/H+ antiporter

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7 January 2021

Searching sequence space to engineer proteins: exponential ensemble mutagenesis

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Amino acid sequence determinants of beta-lactamase structure and activity

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Exploring nonnatural evolutionary pathways by saturation mutagenesis: rapid improvement of protein function

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Genetic analysis of monoclonal antibody and HIV binding sites on the human lymphocyte antigen CD4.

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In vitro scanning saturation mutagenesis of all the specificity determining residues in an antibody binding site

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7 January 2021

Site-directed mutagenesis of double-stranded DNA by the polymerase chain reaction

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7 January 2021

An evolutionary route to xylanase process fitness

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Enhancing the thermal tolerance and gastric performance of a microbial phytase for use as a phosphate-mobilizing monogastric-feed supplement

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7 January 2021

Creation of a productive, highly enantioselective nitrilase through gene site saturation mutagenesis (GSSM).

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7 January 2021

A single point mutation reverses the donor specificity of human blood group B-synthesizing galactosyltransferase

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7 January 2021

Efficient and high fidelity incorporation of dye-terminators by a novel archaeal DNA polymerase mutant

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7 January 2021

High-fidelity amplification using a thermostable DNA polymerase isolated from Pyrococcus furiosus

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7 January 2021

Randomization of genes by PCR mutagenesis

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7 January 2021

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Identifiers

DOI

1 reference

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31 December 2019

1 reference