(Q33223119)

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Biased mutagenesis in the N-terminal region by degenerate oligonucleotide gene shuffling enhances secretory expression of barley alpha-amylase 2 in yeast

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Europe PubMed Central

PubMed publication ID

24 July 2017

Biased mutagenesis in the N-terminal region by degenerate oligonucleotide gene shuffling enhances secretory expression of barley alpha-amylase 2 in yeast (English)

1 reference

Europe PubMed Central

PubMed publication ID

24 July 2017

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Nushin Aghajari

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Nushin Aghajari

4

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Birte Svensson

5

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author name string

Kenji Fukuda

1

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Europe PubMed Central

PubMed publication ID

24 July 2017

Malene H Jensen

2

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Europe PubMed Central

PubMed publication ID

24 July 2017

Richard Haser

3

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Europe PubMed Central

PubMed publication ID

24 July 2017

publication date

9 September 2005

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Europe PubMed Central

PubMed publication ID

24 July 2017

Protein Engineering Design and Selection

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Europe PubMed Central

PubMed publication ID

24 July 2017

18

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Europe PubMed Central

PubMed publication ID

24 July 2017

11

1 reference

Europe PubMed Central

PubMed publication ID

24 July 2017

515-526

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Europe PubMed Central

PubMed publication ID

24 July 2017

Arginine is essential for the alpha-amylase inhibitory activity of the alpha-amylase/subtilisin inhibitor (BASI) from barley seeds

1 reference

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21 January 2018

Barley malt-alpha-amylase. Purification, action pattern, and subsite mapping of isozyme 1 and two members of the isozyme 2 subfamily using p-nitrophenylated maltooligosaccharide substrates

1 reference

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21 January 2018

Tyrosine 105 and threonine 212 at outermost substrate binding subsites -6 and +4 control substrate specificity, oligosaccharide cleavage patterns, and multiple binding modes of barley alpha-amylase 1.

1 reference

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21 January 2018

Crystallography & NMR System: A New Software Suite for Macromolecular Structure Determination

1 reference

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Plants as bioreactors for protein production: avoiding the problem of transgene silencing.

1 reference

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Improved R-factors for diffraction data analysis in macromolecular crystallography

1 reference

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Zero-dose extrapolation as part of macromolecular synchrotron data reduction.

1 reference

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21 January 2018

Lactic acid bacteria as prime candidates for codon optimization.

1 reference

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21 January 2018

Heterologous protein production in methylotrophic yeasts

1 reference

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21 January 2018

SWISS-MODEL and the Swiss-PdbViewer: an environment for comparative protein modeling

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21 January 2018

A revised set of potentials for beta-turn formation in proteins

1 reference

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21 January 2018

Overexpression, purification, and characterization of recombinant barley alpha-amylases 1 and 2 secreted by the methylotrophic yeast Pichia pastoris

1 reference

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21 January 2018

Automatic processing of rotation diffraction data from crystals of initially unknown symmetry and cell constants

1 reference

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21 January 2018

Crystal and molecular structure of barley alpha-amylase

1 reference

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21 January 2018

Molecular structure of a barley alpha-amylase-inhibitor complex: implications for starch binding and catalysis

1 reference

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21 January 2018

Engineering-enhanced protein secretory expression in yeast with application to insulin

1 reference

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21 January 2018

Protein folding stability can determine the efficiency of escape from endoplasmic reticulum quality control

1 reference

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21 January 2018

Secretion efficiency in Saccharomyces cerevisiae of bovine pancreatic trypsin inhibitor mutants lacking disulfide bonds is correlated with thermodynamic stability

1 reference

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21 January 2018

PROCHECK: a program to check the stereochemical quality of protein structures

1 reference

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21 January 2018

Modulation of activity and substrate binding modes by mutation of single and double subsites +1/+2 and -5/-6 of barley alpha-amylase 1.

1 reference

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21 January 2018

Barley alpha-amylase Met53 situated at the high-affinity subsite -2 belongs to a substrate binding motif in the beta-->alpha loop 2 of the catalytic (beta/alpha)8-barrel and is critical for activity and substrate specificity

1 reference

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21 January 2018

Recent advances in heterologous gene expression in Escherichia coli

1 reference

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21 January 2018

Yeast transformation: a model system for the study of recombination

1 reference

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21 January 2018

Yeast recombination: the association between double-strand gap repair and crossing-over

1 reference

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21 January 2018

The structure of barley alpha-amylase isozyme 1 reveals a novel role of domain C in substrate recognition and binding: a pair of sugar tongs

1 reference

https://api.crossref.org/works/10.1093%2FPROTEIN%2FGZI057

21 January 2018

Domain B protruding at the third beta strand of the alpha/beta barrel in barley alpha-amylase confers distinct isozyme-specific properties.

1 reference

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21 January 2018

Directed evolution of proteins for heterologous expression and stability.

1 reference

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21 January 2018

Structure-Expression Relationship of Tumor Necrosis Factor Receptor Mutants That Increase Expression

1 reference

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21 January 2018

Expression of cDNAs encoding barley alpha-amylase 1 and 2 in yeast and characterization of the secreted proteins

1 reference

https://api.crossref.org/works/10.1093%2FPROTEIN%2FGZI057

21 January 2018

C-terminal processing of barley alpha-amylase 1 in malt, aleurone protoplasts, and yeast

1 reference

https://api.crossref.org/works/10.1093%2FPROTEIN%2FGZI057

21 January 2018

Barley alpha-amylase bound to its endogenous protein inhibitor BASI: crystal structure of the complex at 1.9 A resolution

1 reference

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21 January 2018

Specific inhibition of barley alpha-amylase 2 by barley alpha-amylase/subtilisin inhibitor depends on charge interactions and can be conferred to isozyme 1 by mutation

1 reference

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21 January 2018

Identifiers

10.1093/PROTEIN/GZI057

1 reference

Europe PubMed Central

PubMed publication ID

24 July 2017

PubMed publication ID

1 reference

Europe PubMed Central

PubMed publication ID

24 July 2017

ResearchGate publication ID

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