(Q34061238)

30 July 2017

title

Native capillary isoelectric focusing for the separation of protein complex isoforms and subcomplexes (English)

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30 July 2017

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5

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30 July 2017

6

David M Sabatini

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30 July 2017

Bryan R Fonslow

1

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30 July 2017

Seong A Kang

2

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30 July 2017

Daniel R Gestaut

3

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30 July 2017

Beth Graczyk

4

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30 July 2017

John R Yates

7

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30 July 2017

language of work or name

English

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publication date

1 August 2010

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30 July 2017

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30 July 2017

volume

82

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30 July 2017

issue

15

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30 July 2017

page(s)

6643-6651

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Mass spectrometry of membrane transporters reveals subunit stoichiometry and interactions

30 July 2017

cites work

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DEPTOR is an mTOR inhibitor frequently overexpressed in multiple myeloma cells and required for their survival

28 July 2018

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Puzzle of protein complexes in vivo: a present and future challenge for functional proteomics

28 July 2018

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Budding yeast centrosome duplication requires stabilization of Spc29 via Mps1-mediated phosphorylation

28 July 2018

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Joule heating effects and the experimental determination of temperature during CE.

28 July 2018

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mTOR: dissecting regulation and mechanism of action to understand human disease

28 July 2018

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Noncovalent protein tetramers and pentamers with "n" charges yield monomers with n/4 and n/5 charges

28 July 2018

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Empirically controlled mapping of the Caenorhabditis elegans protein-protein interactome network

28 July 2018

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DIABLA: a new screening method for the discovery of protein targets

28 July 2018

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Evolution and dynamics of protein interactions and networks.

28 July 2018

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Phosphoregulation and depolymerization-driven movement of the Dam1 complex do not require ring formation

28 July 2018

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Advances in the analysis of protein phosphorylation.

28 July 2018

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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2920374

Non-covalent capillary coatings for protein separations in capillary electrophoresis

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2920374

Structural biology of RNA polymerase III: mass spectrometry elucidates subcomplex architecture

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2920374

Identification of Protor as a novel Rictor-binding component of mTOR complex-2

28 July 2018

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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2920374

Insulin signalling to mTOR mediated by the Akt/PKB substrate PRAS40

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2920374

Identification of Sin1 as an essential TORC2 component required for complex formation and kinase activity

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2920374

mSin1 is necessary for Akt/PKB phosphorylation, and its isoforms define three distinct mTORC2s

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2920374

Mps1 phosphorylation of Dam1 couples kinetochores to microtubule plus ends at metaphase

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2920374

Detection and analysis of protein-protein interactions in organellar and prokaryotic proteomes by native gel electrophoresis: (Membrane) protein complexes and supercomplexes

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2920374

Spectrophotometry of proteins: Absorption spectra of tyrosine, tryptophan and their mixtures. II. Estimation of tyrosine and tryptophan in proteins

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2920374

Global landscape of protein complexes in the yeast Saccharomyces cerevisiae

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2920374

Characterization of phospholipid-protein interactions by capillary isoelectric focusing with whole-column imaging detection.

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2920374

Proteome survey reveals modularity of the yeast cell machinery

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2920374

Growing roles for the mTOR pathway

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2920374

Tissue proteomics using capillary isoelectric focusing-based multidimensional separations

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2920374

Protein-protein interactions in human disease

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2920374

Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2920374

A highly sensitive method for quantification of myosin light chain phosphorylation by capillary isoelectric focusing with laser-induced fluorescence detection

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2920374

Rictor, a novel binding partner of mTOR, defines a rapamycin-insensitive and raptor-independent pathway that regulates the cytoskeleton

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2920374

A map of the interactome network of the metazoan C. elegans

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2920374

Structure, Function, and Regulation of Budding Yeast Kinetochores

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2920374

GbetaL, a positive regulator of the rapamycin-sensitive pathway required for the nutrient-sensitive interaction between raptor and mTOR

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2920374

mTOR interacts with raptor to form a nutrient-sensitive complex that signals to the cell growth machinery

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2920374

Functional organization of the yeast proteome by systematic analysis of protein complexes

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2920374

A comprehensive analysis of protein-protein interactions in Saccharomyces cerevisiae

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2920374

Direct analysis of protein complexes using mass spectrometry

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2920374

RNA polymerase II holoenzymes and subcomplexes.

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2920374

Structure of the human mTOR complex I and its implications for rapamycin inhibition.

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2920374

A systematic study in CIEF: defining and optimizing experimental parameters critical to method reproducibility and robustness

28 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2920374

Gas phase stabilization of noncovalent protein complexes formed by electrospray ionization

28 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2920374

Quadrupole-time-of-flight mass spectrometer modified for higher-energy dissociation reduces protein assemblies to peptide fragments.

28 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2920374

Human serum albumin interaction with oxaliplatin studied by capillary isoelectric focusing with the whole column imaging detection and spectroscopic method

28 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2920374

Novel multiprotein complexes identified in the hyperthermophilic archaeon Pyrococcus furiosus by non-denaturing fractionation of the native proteome

28 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2920374

Analysis of major protein-protein and protein-metal complexes of erythrocytes directly from cell lysate utilizing capillary electrophoresis mass spectrometry

28 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2920374

Mapping the human protein interactome.

28 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2920374

Micelles protect membrane complexes from solution to vacuum.

28 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2920374

Investigation of interaction between human hemoglobin A0 and platinum anticancer drugs by capillary isoelectric focusing with whole column imaging detection

28 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2920374

A "tagless" strategy for identification of stable protein complexes genome-wide by multidimensional orthogonal chromatographic separation and iTRAQ reagent tracking

28 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2920374

Dynamic kinetic capillary isoelectric focusing: a powerful tool for studying protein-DNA interactions

28 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2920374

SIN1/MIP1 maintains rictor-mTOR complex integrity and regulates Akt phosphorylation and substrate specificity

28 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2920374

Characterization of bovine serum albumin-tryptophan interaction by capillary isoelectric focusing with whole column imaging detection

28 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2920374

Capillary isoelectric focusing and affinity capillary electrophoresis approaches for the determination of binding constants for antibodies to the prion protein

28 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2920374

Use of a native affinity ligand for the detection of G proteins by capillary isoelectric focusing with laser-induced fluorescence detection

28 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2920374

Collisional cooling of large ions in electrospray mass spectrometry

28 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2920374

Raptor, a binding partner of target of rapamycin (TOR), mediates TOR action

28 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2920374

A tandem mass spectrometer for improved transmission and analysis of large macromolecular assemblies.

28 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2920374

Immunoassay of serum alpha(1)-antitrypsin by affinity-probe capillary isoelectric focusing using a fluorescence-labeled recombinant antibody fragment

28 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2920374

A network of protein-protein interactions in yeast

28 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2920374

The cell as a collection of protein machines: preparing the next generation of molecular biologists

28 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2920374

Improving the Performance of a Quadrupole Time-of-Flight Instrument for Macromolecular Mass Spectrometry

28 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2920374

Screening for high-affinity ligands to the Src SH2 domain using capillary isoelectric focusing-electrospray ionization ion trap mass spectrometry

28 October 2018

1 reference

12 December 2020

Improved protein recovery in reversed-phase liquid chromatography by the use of ultrahigh pressures

1 reference

12 December 2020

High-efficiency capillary isoelectric focusing of protein complexes from Escherichia coli cytosolic extracts

1 reference

12 December 2020

Protein losses in ion-exchange and hydrophobic interaction high-performance liquid chromatography

1 reference

12 December 2020

Identification and characterization of protein subcomplexes in yeast

1 reference

12 December 2020

Thermal Dissociation of Multimeric Protein Complexes by Using Nanoelectrospray Mass Spectrometry

1 reference

12 December 2020

High-Resolution Capillary Isoelectric Focusing of Complex Protein Mixtures from Lysates of Microorganisms

1 reference

12 December 2020

Miniaturized capillary isoelectric focusing in plastic microfluidic devices

1 reference

12 December 2020

Effects of catholytes on the mobilization of proteins after capillary isoelectric focusing

1 reference

12 December 2020

Comparison of different capillary isoelectric focusing methods--use of "narrow pH cuts" of carrier ampholytes as original tools to improve resolution

1 reference

12 December 2020

Method for stabilizing protein-ligand complexes in nanoelectrospray ionization mass spectrometry

1 reference

12 December 2020

Method for identifying nonspecific protein-protein interactions in nanoelectrospray ionization mass spectrometry

1 reference

12 December 2020

Separation and Detection of Intact Noncovalent Protein Complexes from Mixtures by On-Line Capillary Isoelectric Focusing-Mass Spectrometry

1 reference

12 December 2020

High resolution analysis of protein phosphorylation using capillary isoelectric focusing - electrospray ionization - mass spectrometry

1 reference

12 December 2020

Identifiers

DOI

10.1021/AC101235K

1 reference

30 July 2017

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30 July 2017

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