(Q34675294)

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Labeling proteins with fluorophore/thioamide Förster resonant energy transfer pairs by combining unnatural amino acid mutagenesis and native chemical ligation

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scholarly article

1 reference

Europe PubMed Central

PubMed publication ID

3 August 2017

Labeling proteins with fluorophore/thioamide Förster resonant energy transfer pairs by combining unnatural amino acid mutagenesis and native chemical ligation (English)

1 reference

Europe PubMed Central

PubMed publication ID

3 August 2017

author name string

Rebecca F Wissner

1

1 reference

Europe PubMed Central

PubMed publication ID

3 August 2017

Solongo Batjargal

2

1 reference

Europe PubMed Central

PubMed publication ID

3 August 2017

Colin M Fadzen

3

1 reference

Europe PubMed Central

PubMed publication ID

3 August 2017

E James Petersson

4

1 reference

Europe PubMed Central

PubMed publication ID

3 August 2017

language of work or name

0 references

publication date

17 April 2013

1 reference

Europe PubMed Central

PubMed publication ID

3 August 2017

Journal of the American Chemical Society

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Europe PubMed Central

PubMed publication ID

3 August 2017

135

1 reference

Europe PubMed Central

PubMed publication ID

3 August 2017

17

1 reference

Europe PubMed Central

PubMed publication ID

3 August 2017

6529-6540

1 reference

Europe PubMed Central

PubMed publication ID

3 August 2017

The conformational ensembles of α-synuclein and tau: combining single-molecule FRET and simulations

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

7 July 2018

Counteracting chemical chaperone effects on the single-molecule α-synuclein structural landscape

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

7 July 2018

Characterization of semisynthetic and naturally Nα-acetylated α-synuclein in vitro and in intact cells: implications for aggregation and cellular properties of α-synuclein

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

7 July 2018

Minimalist probes for studying protein dynamics: thioamide quenching of selectively excitable fluorescent amino acids

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

7 July 2018

Native chemical ligation of thioamide-containing peptides: development and application to the synthesis of labeled α-synuclein for misfolding studies

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

7 July 2018

Semisynthetic, site-specific ubiquitin modification of α-synuclein reveals differential effects on aggregation

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

7 July 2018

Elucidating the role of C-terminal post-translational modifications using protein semisynthesis strategies: α-synuclein phosphorylation at tyrosine 125.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

7 July 2018

Exogenous α-synuclein fibrils induce Lewy body pathology leading to synaptic dysfunction and neuron death

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

7 July 2018

An evaluation of peptide-bond isosteres

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

7 July 2018

Electron paramagnetic resonance spectroscopy measures the distance between the external β-strands of folded α-synuclein in amyloid fibrils

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

7 July 2018

In vivo demonstration that alpha-synuclein oligomers are toxic

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

7 July 2018

Single molecule characterization of α-synuclein in aggregation-prone states

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

7 July 2018

Modulation of p-cyanophenylalanine fluorescence by amino acid side chains and rational design of fluorescence probes of alpha-helix formation

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

7 July 2018

α-Synuclein: membrane interactions and toxicity in Parkinson's disease

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

7 July 2018

Mechanisms of quenching of Alexa fluorophores by natural amino acids

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

7 July 2018

Photophysics of a Fluorescent Non-natural Amino Acid: p-Cyanophenylalanine

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

7 July 2018

The role of conformational entropy in molecular recognition by calmodulin

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

7 July 2018

Detection of transient interchain interactions in the intrinsically disordered protein alpha-synuclein by NMR paramagnetic relaxation enhancement

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

7 July 2018

Encoding multiple unnatural amino acids via evolution of a quadruplet-decoding ribosome

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

7 July 2018

Determination of the free energy landscape of alpha-synuclein using spin label nuclear magnetic resonance measurements

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

7 July 2018

Efficient method of circumventing insolubility problems with fully protected peptide carboxylates via in situ direct thioesterification reactions

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

7 July 2018

Measuring distances within unfolded biopolymers using fluorescence resonance energy transfer: The effect of polymer chain dynamics on the observed fluorescence resonance energy transfer efficiency

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

7 July 2018

Interpretation of p-cyanophenylalanine fluorescence in proteins in terms of solvent exposure and contribution of side-chain quenchers: a combined fluorescence, IR and molecular dynamics study

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

7 July 2018

Inclusion formation and neuronal cell death through neuron-to-neuron transmission of alpha-synuclein

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

7 July 2018

Probing protein folding using site-specifically encoded unnatural amino acids as FRET donors with tryptophan.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

7 July 2018

How accurate are polymer models in the analysis of Förster resonance energy transfer experiments on proteins?

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

7 July 2018

Interplay of alpha-synuclein binding and conformational switching probed by single-molecule fluorescence.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

7 July 2018

A general and efficient method for the site-specific dual-labeling of proteins for single molecule fluorescence resonance energy transfer

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

7 July 2018

The fold of alpha-synuclein fibrils

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

7 July 2018

Fluorescent protein FRET: the good, the bad and the ugly

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

7 July 2018

Amide-to-E-olefin versus amide-to-ester backbone H-bond perturbations: Evaluating the O-O repulsion for extracting H-bond energies

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

7 July 2018

Protein ligation: an enabling technology for the biophysical analysis of proteins

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

7 July 2018

Probing protein folding and conformational transitions with fluorescence.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

7 July 2018

Synthesis of proteins by native chemical ligation using Fmoc-based chemistry

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

7 July 2018

Glycine as a D-amino acid surrogate in the K(+)-selectivity filter

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

7 July 2018

Alpha-synuclein structures from fluorescence energy-transfer kinetics: implications for the role of the protein in Parkinson's disease

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

7 July 2018

Semisynthesis of proteins by expressed protein ligation

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

7 July 2018

Novel aspects of calmodulin target recognition and activation

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

7 July 2018

Expanding the genetic code of Escherichia coli

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

7 July 2018

Fibrils formed in vitro from alpha-synuclein and two mutant forms linked to Parkinson's disease are typical amyloid

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

7 July 2018

alpha-synuclein fibrillogenesis is nucleation-dependent. Implications for the pathogenesis of Parkinson's disease

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

7 July 2018

Single-pair fluorescence resonance energy transfer on freely diffusing molecules: observation of Förster distance dependence and subpopulations

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

7 July 2018

Alpha-synuclein in Lewy bodies

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

7 July 2018

Calcium-calmodulin modulation of the olfactory cyclic nucleotide-gated cation channel

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

7 July 2018

Plasmid transformation of Escherichia coli and other bacteria

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

7 July 2018

Toxic prefibrillar α-synuclein amyloid oligomers adopt a distinctive antiparallel β-sheet structure.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

26 October 2018

Thioamides as fluorescence quenching probes: minimalist chromophores to monitor protein dynamics

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

26 October 2018

Peptide and protein thioester synthesis via N-->S acyl transfer.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

26 October 2018

A facile system for genetic incorporation of two different noncanonical amino acids into one protein in Escherichia coli.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

26 October 2018

A convenient method for genetic incorporation of multiple noncanonical amino acids into one protein in Escherichia coli

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

26 October 2018

A pyrrolysine analogue for site-specific protein ubiquitination

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

26 October 2018

An enhanced system for unnatural amino acid mutagenesis in E. coli

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

26 October 2018

Conformational distribution of a 14-residue peptide in solution: a fluorescence resonance energy transfer study.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

26 October 2018

A genetically encoded fluorescent amino acid.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

26 October 2018

Tertiary contact formation in alpha-synuclein probed by electron transfer.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

26 October 2018

E-olefin dipeptide isostere incorporation into a polypeptide backbone enables hydrogen bond perturbation: probing the requirements for Alzheimer's amyloidogenesis.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

26 October 2018

Site-specific dimensions across a highly denatured protein; a single molecule study

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

26 October 2018

Structure of calmodulin complexed with an olfactory CNG channel fragment and role of the central linker: residual dipolar couplings to evaluate calmodulin binding modes outside the kinase family.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

26 October 2018

Direct production of proteins with N-terminal cysteine for site-specific conjugation

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

26 October 2018

Addition of p-azido-L-phenylalanine to the genetic code of Escherichia coli

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

26 October 2018

Trimethylamine-N-oxide-induced folding of alpha-synuclein.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

26 October 2018

Thiopeptide Synthesis. alpha-Amino Thionoacid Derivatives of Nitrobenzotriazole as Thioacylating Agents

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

26 October 2018

Evidence for a partially folded intermediate in alpha-synuclein fibril formation

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3721677

26 October 2018

Comparative studies on thermostability of calmodulin, skeletal muscle troponin C and their tryptic fragments

1 reference

https://pubmed.ncbi.nlm.nih.gov/23594264

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Photophysics and Mechanisms of Intramolecular Electronic Energy Transfer in Bichromophoric Molecular Systems: Solution and Supersonic Jet Studies

1 reference

https://pubmed.ncbi.nlm.nih.gov/23594264

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Tyrosine fluorescence of ram testis and octopus calmodulins. Effects of calcium, magnesium, and ionic strength

1 reference

https://pubmed.ncbi.nlm.nih.gov/23594264

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Genetically Encoded 1,2-Aminothiols Facilitate Rapid and Site-Specific Protein Labeling via a Bio-orthogonal Cyanobenzothiazole Condensation

1 reference

https://pubmed.ncbi.nlm.nih.gov/23594264

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Fluorescence energy transfer analysis of calmodulin-peptide complexes

1 reference

https://pubmed.ncbi.nlm.nih.gov/23594264

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Thermodynamics of target peptide recognition by calmodulin and a calmodulin analogue: implications for the role of the central linker

1 reference

https://pubmed.ncbi.nlm.nih.gov/23594264

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Thioamide quenching of intrinsic protein fluorescence

1 reference

https://pubmed.ncbi.nlm.nih.gov/23594264

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Association of alpha-synuclein and mutants with lipid membranes: spin-label ESR and polarized IR

1 reference

https://pubmed.ncbi.nlm.nih.gov/23594264

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Expanding the Genetic Code

1 reference

https://pubmed.ncbi.nlm.nih.gov/23594264

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Towards elucidation of the role of ubiquitination in the pathogenesis of Parkinson's disease with semisynthetic ubiquitinated α-synuclein

1 reference

https://pubmed.ncbi.nlm.nih.gov/23594264

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Large-scale purification and characterization of calmodulin from ram testis: its metal-ion-dependent conformers

1 reference

https://pubmed.ncbi.nlm.nih.gov/23594264

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Time-resolved fluorescence study of VU-9 calmodulin, an engineered calmodulin possessing a single tryptophan residue

1 reference

https://pubmed.ncbi.nlm.nih.gov/23594264

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Thermodynamic analysis of calcium and magnesium binding to calmodulin

1 reference

https://pubmed.ncbi.nlm.nih.gov/23594264

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Chemical protein synthesis

1 reference

https://pubmed.ncbi.nlm.nih.gov/23594264

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Identifiers

10.1021/JA4005943

1 reference

Europe PubMed Central

PubMed publication ID

3 August 2017

PMC publication ID

1 reference

Europe PubMed Central

PubMed publication ID

3 August 2017

PubMed publication ID

1 reference

Europe PubMed Central

PubMed publication ID

3 August 2017

ResearchGate publication ID

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