(Q35057349)

6 August 2017

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title

Principles: extending the utility of [35S]GTP gamma S binding assays (English)

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6 August 2017

Graeme Milligan

1

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6 August 2017

language of work or name

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1 February 2003

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Trends in Pharmacological Sciences

6 August 2017

published in

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6 August 2017

volume

24

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6 August 2017

issue

2

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6 August 2017

page(s)

87-90

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Analysis of G protein activation in Sf9 and mammalian cells by agonist-promoted [35S]GTP gamma S binding

6 August 2017

cites work

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7 January 2021

AT1 receptor mutant lacking heterotrimeric G protein coupling activates the Src-Ras-ERK pathway without nuclear translocation of ERKs.

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7 January 2021

Reporter-gene systems for the study of G-protein-coupled receptors.

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7 January 2021

Differential coupling of the sphingosine 1-phosphate receptors Edg-1, Edg-3, and H218/Edg-5 to the G(i), G(q), and G(12) families of heterotrimeric G proteins

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7 January 2021

Homologous and heterologous uncoupling of muscarinic M(3) and alpha(1B) adrenoceptors to Galpha(q/11) in SH-SY5Y human neuroblastoma cells

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7 January 2021

Endogenous G protein-coupled receptor kinase 6 Regulates M3 muscarinic acetylcholine receptor phosphorylation and desensitization in human SH-SY5Y neuroblastoma cells

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7 January 2021

Design and use of C-terminal minigene vectors for studying role of heterotrimeric G proteins

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7 January 2021

Selective inhibition of heterotrimeric Gs signaling. Targeting the receptor-G protein interface using a peptide minigene encoding the Galpha(s) carboxyl terminus

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7 January 2021

M1 muscarinic receptor signaling in mouse hippocampus and cortex.

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7 January 2021

Differential activation of Gq/11 and Gi(3) proteins at 5-hydroxytryptamine(2C) receptors revealed by antibody capture assays: influence of receptor reserve and relationship to agonist-directed trafficking

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7 January 2021

Antibody capture assay reveals bell-shaped concentration-response isotherms for h5-HT(1A) receptor-mediated Galpha(i3) activation: conformational selection by high-efficacy agonists, and relationship to trafficking of receptor signaling

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7 January 2021

Neuroanatomical localization of receptor-activated G proteins in brain

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G(q/11) and G(i/o) activation profiles in CHO cells expressing human muscarinic acetylcholine receptors: dependence on agonist as well as receptor-subtype

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7 January 2021

Measurement of agonist-dependent and -independent signal initiation of alpha(1b)-adrenoceptor mutants by direct analysis of guanine nucleotide exchange on the G protein galpha(11).

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7 January 2021

Construction and analysis of function of G protein-coupled receptor-G protein fusion proteins

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7 January 2021

Analytical pharmacology of G protein-coupled receptors by stoichiometric expression of the receptor and G(alpha) protein subunits

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7 January 2021

Examining the efficiency of receptor/G-protein coupling with a cleavable beta2-adrenoceptor-gsalpha fusion protein

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7 January 2021

Coordinated agonist regulation of receptor and G protein palmitoylation and functional rescue of palmitoylation-deficient mutants of the G protein G11alpha following fusion to the alpha1b-adrenoreceptor: palmitoylation of G11alpha is not required fo

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7 January 2021

Effective information transfer from the alpha 1b-adrenoceptor to Galpha 11 requires both beta/gamma interactions and an aromatic group four amino acids from the C terminus of the G protein

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7 January 2021

Generation and analysis of constitutively active and physically destabilized mutants of the human beta(1)-adrenoceptor

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7 January 2021

Analysis of agonist function at fusion proteins between the IP prostanoid receptor and cognate, unnatural and chimaeric G-proteins

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7 January 2021

Molecular analysis of beta(2)-adrenoceptor coupling to G(s)-, G(i)-, and G(q)-proteins.

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7 January 2021

10.1016/S0165-6147(02)00027-5

Identifiers

DOI

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6 August 2017

1 reference