(Q38295567)

1 September 2017

title

Construction and analysis of function of G protein-coupled receptor-G protein fusion proteins (English)

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G protein-coupled receptor

1 September 2017

main subject

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author name string

Graeme Milligan

series ordinal

1

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1 September 2017

language of work or name

English

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publication date

1 January 2002

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1 September 2017

published in

Methods in Enzymology

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1 September 2017

volume

343

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1 September 2017

page(s)

260-273

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Binding, gating, affinity and efficacy: the interpretation of structure-activity relationships for agonists and of the effects of mutating receptors

1 September 2017

cites work

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2802%2943141-2

7 January 2021

GPCR-Galpha fusion proteins: molecular analysis of receptor-G-protein coupling

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2802%2943141-2

7 January 2021

Insights into ligand pharmacology using receptor-G-protein fusion proteins

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https://api.crossref.org/works/10.1016%2FS0076-6879%2802%2943141-2

7 January 2021

Measurement of agonist efficacy using an alpha2A-adrenoceptor-Gi1alpha fusion protein

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https://api.crossref.org/works/10.1016%2FS0076-6879%2802%2943141-2

7 January 2021

An Asp79Asn mutation of the alpha2A-adrenoceptor interferes equally with agonist activation of individual Gialpha-family G protein subtypes

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2802%2943141-2

7 January 2021

Modulation of relative intrinsic activity of agonists at the alpha-2A adrenoceptor by mutation of residue 351 of G protein gi1alpha

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2802%2943141-2

7 January 2021

Restricting mobility of Gsalpha relative to the beta2-adrenoceptor enhances adenylate cyclase activity by reducing Gsalpha GTPase activity

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2802%2943141-2

7 January 2021

Examining the efficiency of receptor/G-protein coupling with a cleavable beta2-adrenoceptor-gsalpha fusion protein

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2802%2943141-2

7 January 2021

Functional analysis of a human A(1) adenosine receptor/green fluorescent protein/G(i1)alpha fusion protein following stable expression in CHO cells.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2802%2943141-2

7 January 2021

Analysis of agonist function at fusion proteins between the IP prostanoid receptor and cognate, unnatural and chimaeric G-proteins

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2802%2943141-2

7 January 2021

Different effects of Gsalpha splice variants on beta2-adrenoreceptor-mediated signaling. The Beta2-adrenoreceptor coupled to the long splice variant of Gsalpha has properties of a constitutively active receptor

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2802%2943141-2

7 January 2021

Quantitative analysis of formyl peptide receptor coupling to g(i)alpha(1), g(i)alpha(2), and g(i)alpha(3).

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2802%2943141-2

7 January 2021

Activation of the beta(2)-adrenergic receptor-Galpha(s) complex leads to rapid depalmitoylation and inhibition of repalmitoylation of both the receptor and Galpha(s).

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2802%2943141-2

7 January 2021

Comparative analysis of the efficacy of A1 adenosine receptor activation of Gi/o alpha G proteins following coexpression of receptor and G protein and expression of A1 adenosine receptor-Gi/o alpha fusion proteins

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2802%2943141-2

7 January 2021

N-Myristoylation and betagamma play roles beyond anchorage in the palmitoylation of the G protein alpha(o) subunit

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2802%2943141-2

7 January 2021

Rescue of functional interactions between the alpha2A-adrenoreceptor and acylation-resistant forms of Gi1alpha by expressing the proteins from chimeric open reading frames.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2802%2943141-2

7 January 2021

Measurement of agonist-induced guanine nucleotide turnover by the G-protein Gi1alpha when constrained within an alpha2A-adrenoceptor-Gi1alpha fusion protein

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2802%2943141-2

7 January 2021

Kinetics of ternary complex formation with fusion proteins composed of the A(1)-adenosine receptor and G protein alpha-subunits.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2802%2943141-2

7 January 2021

G proteins: transducers of receptor-generated signals

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https://api.crossref.org/works/10.1016%2FS0076-6879%2802%2943141-2

7 January 2021

Receptor-stimulated hydrolysis of guanosine 5'-triphosphate in membrane preparations

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https://api.crossref.org/works/10.1016%2FS0076-6879%2802%2943141-2

7 January 2021

Measurement of receptor-stimulated guanosine 5'-O-(gamma-thio)triphosphate binding by G proteins

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2802%2943141-2

7 January 2021

Modulation of 5-HT1A receptor signalling by point-mutation of cysteine351 in the rat Galpha(o) protein

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2802%2943141-2

7 January 2021

Ligand-receptor interactions as controlled by wild-type and mutant Thr(370)Lys alpha2B-adrenoceptor-Galpha15 fusion proteins

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2802%2943141-2

7 January 2021

Coupling of the muscarinic m2 receptor to G protein-activated K(+) channels via Galpha(z) and a receptor-Galpha(z) fusion protein. Fusion between the receptor and Galpha(z) eliminates catalytic (collision) coupling.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2802%2943141-2

7 January 2021

The G protein alpha subunit has a key role in determining the specificity of coupling to, but not the activation of, G protein-gated inwardly rectifying K(+) channels

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2802%2943141-2

7 January 2021

Agonist occupation of an alpha2A-adrenoreceptor-Gi1alpha fusion protein results in activation of both receptor-linked and endogenous Gi proteins. Comparisons of their contributions to GTPase activity and signal transduction and analysis of receptor-

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2802%2943141-2

7 January 2021

A point mutation of the alpha 2-adrenoceptor that blocks coupling to potassium but not calcium currents

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2802%2943141-2

7 January 2021

How receptors talk to trimeric G proteins

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2802%2943141-2

7 January 2021

Structure-function analysis of muscarinic receptors and their associated G proteins

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2802%2943141-2

7 January 2021

Interactions of the alpha2A-adrenoceptor with multiple Gi-family G-proteins: studies with pertussis toxin-resistant G-protein mutants

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2802%2943141-2

7 January 2021

Hydrophobicity of residue351 of the G protein Gi1 alpha determines the extent of activation by the alpha 2A-adrenoceptor.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2802%2943141-2

7 January 2021

Quantitative analysis of a cysteine351glycine mutation in the G protein Gi1alpha: effect on alpha2A-adrenoceptor-Gi1alpha fusion protein activation

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2802%2943141-2

7 January 2021

Chimaeric G alpha proteins: their potential use in drug discovery

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2802%2943141-2

7 January 2021

Efficient signal transduction by a chimeric yeast-mammalian G protein alpha subunit Gpa1-Gsalpha covalently fused to the yeast receptor Ste2.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2802%2943141-2

7 January 2021

10.1016/S0076-6879(02)43141-2

Identifiers

DOI

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1 September 2017

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