(Q30657902)

26 June 2017

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title

Analytical pharmacology of G protein-coupled receptors by stoichiometric expression of the receptor and G(alpha) protein subunits (English)

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G protein-coupled receptor

26 June 2017

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Wurch T

1

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26 June 2017

Pauwels PJ

series ordinal

2

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26 June 2017

language of work or name

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1 January 2001

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Journal of Pharmacological and Toxicological Methods

26 June 2017

published in

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26 June 2017

volume

45

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26 June 2017

issue

1

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26 June 2017

page(s)

3-16

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Cellular signaling by an agonist-activated receptor/Gs alpha fusion protein

26 June 2017

cites work

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7 January 2021

Human beta2-adrenergic receptor/GS alpha fusion protein, expressed in 2 ras-dependent murine carcinoma cell lines, prevents tumor growth in syngeneic mice

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Functional analysis of a human A(1) adenosine receptor/green fluorescent protein/G(i1)alpha fusion protein following stable expression in CHO cells.

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7 January 2021

G-Protein coupled receptors: models, mutagenesis, and drug design

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7 January 2021

Agonist occupation of an alpha2A-adrenoreceptor-Gi1alpha fusion protein results in activation of both receptor-linked and endogenous Gi proteins. Comparisons of their contributions to GTPase activity and signal transduction and analysis of receptor-

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7 January 2021

Quantitative analysis of a cysteine351glycine mutation in the G protein Gi1alpha: effect on alpha2A-adrenoceptor-Gi1alpha fusion protein activation

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7 January 2021

Modulation of 5-HT1A receptor signalling by point-mutation of cysteine351 in the rat Galpha(o) protein

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7 January 2021

Modulation of 5-HT(1A) receptor activation by its interaction with wild-type and mutant g(alphai3) proteins

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7 January 2021

Analysis of agonist function at fusion proteins between the IP prostanoid receptor and cognate, unnatural and chimaeric G-proteins

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7 January 2021

Expression of two human beta-adrenergic receptors in Escherichia coli: functional interaction with two forms of the stimulatory G protein

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7 January 2021

The G-protein betagamma complex

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7 January 2021

Contribution of receptor/G protein signaling to cell growth and transformation.

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7 January 2021

Role of subunit diversity in signaling by heterotrimeric G proteins

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7 January 2021

Modulation of relative intrinsic activity of agonists at the alpha-2A adrenoceptor by mutation of residue 351 of G protein gi1alpha

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7 January 2021

Agonist-receptor efficacy. I: Mechanisms of efficacy and receptor promiscuity.

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7 January 2021

Differences between natural and recombinant G protein-coupled receptor systems with varying receptor/G protein stoichiometry

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7 January 2021

7TM receptors: the splicing on the cake

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7 January 2021

Quantitative stoichiometry of the proteins of the stimulatory arm of the adenylyl cyclase cascade in neuroblastoma x glioma hybrid, NG108-15 cells

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7 January 2021

Structural determinants for activation of the alpha-subunit of a heterotrimeric G protein

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7 January 2021

Constitutive activity of receptors coupled to guanine nucleotide regulatory proteins

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7 January 2021

G proteins endogenously expressed in Sf 9 cells: interactions with mammalian histamine receptors

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7 January 2021

From epinephrine to cyclic AMP.

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7 January 2021

Efficient signal transduction by a chimeric yeast-mammalian G protein alpha subunit Gpa1-Gsalpha covalently fused to the yeast receptor Ste2.

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7 January 2021

Insights into ligand pharmacology using receptor-G-protein fusion proteins

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7 January 2021

The dynamic role of palmitoylation in signal transduction

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7 January 2021

The 2.2 A crystal structure of transducin-alpha complexed with GTP gamma S

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7 January 2021

Complex information processing by the transmembrane signaling system involving G proteins

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7 January 2021

G alpha 15 and G alpha 16 couple a wide variety of receptors to phospholipase C

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7 January 2021

Crystal structure of rhodopsin: A G protein-coupled receptor

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7 January 2021

Diverse signalling by 5-hydroxytryptamine (5-HT) receptors

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7 January 2021

Heterogeneous ligand-mediated Ca(++) responses at wt and mutant alpha(2A)-adrenoceptors suggest multiple ligand activation binding sites at the alpha(2A)-adrenoceptor

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7 January 2021

Disparate ligand-mediated Ca(2+) responses by wild-type, mutant Ser(200)Ala and Ser(204)Ala alpha(2A)-adrenoceptor: G(alpha15) fusion proteins: evidence for multiple ligand-activation binding sites

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7 January 2021

Agonist efficacy at the alpha2A-adrenoceptor:G alpha15 fusion protein: an analysis based on Ca2+ responses

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7 January 2021

Ligand-receptor interactions as controlled by wild-type and mutant Thr(370)Lys alpha2B-adrenoceptor-Galpha15 fusion proteins

1 reference

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7 January 2021

Analysis of ligand activation of alpha 2-adrenoceptor subtypes under conditions of equal G alpha protein stoichiometry

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7 January 2021

alpha(2A)-adrenoceptor: G(alphai1) protein-mediated pertussis toxin-resistant attenuation of G(s) coupling to the cyclic AMP pathway.

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7 January 2021

Pertussis toxin in the analysis of receptor mechanisms

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7 January 2021

Loss of activation of Gs but not Gi following expression of an alpha2A-adrenoceptor-Gi1alpha fusion protein

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7 January 2021

Reconstitution of beta2-adrenoceptor-GTP-binding-protein interaction in Sf9 cells--high coupling efficiency in a beta2-adrenoceptor-G(s alpha) fusion protein

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7 January 2021

Examining the efficiency of receptor/G-protein coupling with a cleavable beta2-adrenoceptor-gsalpha fusion protein

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7 January 2021

GPCR-Galpha fusion proteins: molecular analysis of receptor-G-protein coupling

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7 January 2021

Different effects of Gsalpha splice variants on beta2-adrenoreceptor-mediated signaling. The Beta2-adrenoreceptor coupled to the long splice variant of Gsalpha has properties of a constitutively active receptor

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7 January 2021

Differences in G-protein activation by mu- and delta-opioid, and cannabinoid, receptors in rat striatum

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7 January 2021

Fusion of beta 2-adrenergic receptor to G alpha s in mammalian cells: identification of a specific signal transduction species not characteristic of constitutive activation or precoupling

1 reference

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7 January 2021

Coupling efficacy and selectivity of the human mu-opioid receptor expressed as receptor-Galpha fusion proteins in Escherichia coli.

1 reference

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7 January 2021

Coupling of the muscarinic m2 receptor to G protein-activated K(+) channels via Galpha(z) and a receptor-Galpha(z) fusion protein. Fusion between the receptor and Galpha(z) eliminates catalytic (collision) coupling.

1 reference

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7 January 2021

Kinetics of ternary complex formation with fusion proteins composed of the A(1)-adenosine receptor and G protein alpha-subunits.

1 reference

https://api.crossref.org/works/10.1016%2FS1056-8719%2801%2900126-5

7 January 2021

An Asp79Asn mutation of the alpha2A-adrenoceptor interferes equally with agonist activation of individual Gialpha-family G protein subtypes

1 reference

https://api.crossref.org/works/10.1016%2FS1056-8719%2801%2900126-5

7 January 2021

Quantitative analysis of formyl peptide receptor coupling to g(i)alpha(1), g(i)alpha(2), and g(i)alpha(3).

1 reference

https://api.crossref.org/works/10.1016%2FS1056-8719%2801%2900126-5

7 January 2021

Restricting mobility of Gsalpha relative to the beta2-adrenoceptor enhances adenylate cyclase activity by reducing Gsalpha GTPase activity

1 reference

https://api.crossref.org/works/10.1016%2FS1056-8719%2801%2900126-5

7 January 2021

Characterization of G-protein alpha subunits in the Gq class: expression in murine tissues and in stromal and hematopoietic cell lines

1 reference

https://api.crossref.org/works/10.1016%2FS1056-8719%2801%2900126-5

7 January 2021

Measurement of agonist-induced guanine nucleotide turnover by the G-protein Gi1alpha when constrained within an alpha2A-adrenoceptor-Gi1alpha fusion protein

1 reference

https://api.crossref.org/works/10.1016%2FS1056-8719%2801%2900126-5

7 January 2021

Measurement of agonist efficacy using an alpha2A-adrenoceptor-Gi1alpha fusion protein

1 reference

https://api.crossref.org/works/10.1016%2FS1056-8719%2801%2900126-5

7 January 2021

Rescue of functional interactions between the alpha2A-adrenoreceptor and acylation-resistant forms of Gi1alpha by expressing the proteins from chimeric open reading frames.

1 reference

https://api.crossref.org/works/10.1016%2FS1056-8719%2801%2900126-5

7 January 2021

Comparative analysis of the efficacy of A1 adenosine receptor activation of Gi/o alpha G proteins following coexpression of receptor and G protein and expression of A1 adenosine receptor-Gi/o alpha fusion proteins

1 reference

https://api.crossref.org/works/10.1016%2FS1056-8719%2801%2900126-5

7 January 2021

G-protein activation by putative antagonists at mutant Thr373Lys alpha2A adrenergic receptors

1 reference

https://api.crossref.org/works/10.1016%2FS1056-8719%2801%2900126-5

7 January 2021

10.1016/S1056-8719(01)00126-5

Identifiers

DOI

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26 June 2017

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