(Q35927569)

11 August 2017

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title

Protein expression and refolding--a practical guide to getting the most out of inclusion bodies (English)

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11 August 2017

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2

Stephen P Bottomley

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11 August 2017

Lisa D Cabrita

1

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11 August 2017

language of work or name

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1 January 2004

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Biotechnology Annual Review

11 August 2017

published in

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11 August 2017

volume

10

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11 August 2017

page(s)

31-50

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Principles that govern the folding of protein chains

11 August 2017

cites work

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7 January 2021

Is there a unifying mechanism for protein folding?

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7 January 2021

Protein misfolding, evolution and disease

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7 January 2021

Recombinant protein expression in Escherichia coli

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7 January 2021

Genetic design for facilitated production and recovery of recombinant proteins in Escherichia coli.

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7 January 2021

Advances in Escherichia coli production of therapeutic proteins

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7 January 2021

Expression and purification of the human homeodomain oncoprotein HOX11.

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7 January 2021

Stress-induced aggregation profiles of GST-alpha-synuclein fusion proteins: role of the C-terminal acidic tail of alpha-synuclein in protein thermosolubility and stability

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7 January 2021

Purification and biophysical characterization of a minimal functional domain and of an N-terminal Zn2+-binding fragment from the human papillomavirus type 16 E6 protein

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7 January 2021

Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase

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7 January 2021

Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems

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7 January 2021

Formation of soluble inclusion bodies by hpv e6 oncoprotein fused to maltose-binding protein

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7 January 2021

Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein

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7 January 2021

Solubility of proteins isolated from inclusion bodies is enhanced by fusion to maltose-binding protein or thioredoxin

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7 January 2021

Crystal structures of fusion proteins with large-affinity tags

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7 January 2021

Ribonuclease S-peptide as a carrier in fusion proteins.

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7 January 2021

Characterization of a cellobiose phosphorylase from a hyperthermophilic eubacterium, Thermotoga maritima MSB8.

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7 January 2021

Molecular characterization of a phosphoenolpyruvate carboxylase from a thermophilic cyanobacterium, Synechococcus vulcanus with unusual allosteric properties

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7 January 2021

Expression and one-step purification of a developmentally regulated protein from Dictyostelium discoideum

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7 January 2021

The native metastable fold of C1-inhibitor is stabilized by disulfide bonds

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7 January 2021

Chitinases of the avian malaria parasite Plasmodium gallinaceum, a class of enzymes necessary for parasite invasion of the mosquito midgut.

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7 January 2021

Molecular Cloning of a cDNA Encoding Copper/Zinc Superoxide Dismutase from Papaya Fruit and Overexpression in Escherichia coli.

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7 January 2021

Cell-Free Synthesis of Rat Growth Hormone

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7 January 2021

A continuous cell-free translation system capable of producing polypeptides in high yield

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7 January 2021

A semicontinuous prokaryotic coupled transcription/translation system using a dialysis membrane

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7 January 2021

Expression-independent consumption of substrates in cell-free expression system from Escherichia coli

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7 January 2021

A wheat germ cell-free system is a novel way to screen protein folding and function

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7 January 2021

A highly efficient and robust cell-free protein synthesis system prepared from wheat embryos: plants apparently contain a suicide system directed at ribosomes

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7 January 2021

Prolonged cell-free protein synthesis in a batch system using wheat germ extract

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7 January 2021

Design of high-throughput methods of protein production for structural biology

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7 January 2021

Refolding of therapeutic proteins produced in Escherichia coli as inclusion bodies

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https://api.crossref.org/works/10.1016%2FS1387-2656%2804%2910002-1

7 January 2021

Effect of inclusion body contaminants on the oxidative renaturation of hen egg white lysozyme

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https://api.crossref.org/works/10.1016%2FS1387-2656%2804%2910002-1

7 January 2021

Cyanate formation in solutions of urea. I. Calculation of cyanate concentrations at different temperature and pH.

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7 January 2021

Nucleotide binding to human uncoupling protein-2 refolded from bacterial inclusion bodies

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7 January 2021

The phage lambda terminase enzyme: 2. Refolding of the gpNu1 subunit from the detergent-denatured and guanidinium hydrochloride-denatured state yields different oligomerization states and altered protein stabilities

1 reference

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7 January 2021

Characterization of a truncated form of recombinant porcine growth hormone generated in vitro during solubilization of inclusion bodies.

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7 January 2021

Refolding, structural transition and spermatozoa-binding of recombinant bonnet monkey (Macaca radiata) zona pellucida glycoprotein-C expressed in Escherichia coli

1 reference

https://api.crossref.org/works/10.1016%2FS1387-2656%2804%2910002-1

7 January 2021

Solubilization of recombinant ovine growth hormone with retention of native-like secondary structure and its refolding from the inclusion bodies of Escherichia coli

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7 January 2021

Purification of correctly oxidized MHC class I heavy-chain molecules under denaturing conditions: a novel strategy exploiting disulfide assisted protein folding.

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7 January 2021

Expression and characterization of recombinant canine IL-13 receptor alpha2 protein and its biological activity in vitro

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7 January 2021

alpha-lytic protease precursor: characterization of a structured folding intermediate

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7 January 2021

Production and purification of refolded recombinant human IL-7 from inclusion bodies

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7 January 2021

Chromatographic methods for the isolation of, and refolding of proteins from, Escherichia coli inclusion bodies

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7 January 2021

Evidence for a molten globule state as a general intermediate in protein folding

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7 January 2021

High recovery of prochymosin from inclusion bodies using controlled air oxidation

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7 January 2021

Oxidative renaturation of hen egg-white lysozyme. Folding vs aggregation

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7 January 2021

Control of aggregation in protein refolding: the temperature-leap tactic

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7 January 2021

A kinetic study of the competition between renaturation and aggregation during the refolding of denatured-reduced egg white lysozyme.

1 reference

https://api.crossref.org/works/10.1016%2FS1387-2656%2804%2910002-1

7 January 2021

Molecular cloning and characterization of the alkaline ceramidase from Pseudomonas aeruginosa PA01.

1 reference

https://api.crossref.org/works/10.1016%2FS1387-2656%2804%2910002-1

7 January 2021

Production and characterization of bioactive recombinant resistin in Escherichia coli

1 reference

https://api.crossref.org/works/10.1016%2FS1387-2656%2804%2910002-1

7 January 2021

Refolding from denatured inclusion bodies, purification to homogeneity and simplified assay of MGDG synthases from land plants

1 reference

https://api.crossref.org/works/10.1016%2FS1387-2656%2804%2910002-1

7 January 2021

Renaturation of 1-aminocyclopropane-1-carboxylate synthase expressed in Escherichia coli in the form of inclusion bodies into a dimeric and catalytically active enzyme

1 reference

https://api.crossref.org/works/10.1016%2FS1387-2656%2804%2910002-1

7 January 2021

Role of Lys335 in the metastability and function of inhibitory serpins

1 reference

https://api.crossref.org/works/10.1016%2FS1387-2656%2804%2910002-1

7 January 2021

Structure of folding intermediates at pH 4.0 and native state of microbial transglutaminase.

1 reference

https://api.crossref.org/works/10.1016%2FS1387-2656%2804%2910002-1

7 January 2021

Protein engineering of chimeric Serpins: an investigation into effects of the serpin scaffold and reactive centre loop length

1 reference

https://api.crossref.org/works/10.1016%2FS1387-2656%2804%2910002-1

7 January 2021

Refolding of Escherichia coli produced membrane protein inclusion bodies immobilised by nickel chelating chromatography

1 reference

https://api.crossref.org/works/10.1016%2FS1387-2656%2804%2910002-1

7 January 2021

Large scale production of biologically active Escherichia coli glutamyl-tRNA reductase from inclusion bodies

1 reference

https://api.crossref.org/works/10.1016%2FS1387-2656%2804%2910002-1

7 January 2021

Purification of the Caenorhabditis elegans transposase Tc1A refolded during gel filtration chromatography

1 reference

https://api.crossref.org/works/10.1016%2FS1387-2656%2804%2910002-1

7 January 2021

Urea gradient size-exclusion chromatography enhanced the yield of lysozyme refolding

1 reference

https://api.crossref.org/works/10.1016%2FS1387-2656%2804%2910002-1

7 January 2021

Effect of column dimensions and flow rates on size-exclusion refolding of beta-lactamase

1 reference

https://api.crossref.org/works/10.1016%2FS1387-2656%2804%2910002-1

7 January 2021

Refolding chromatography with immobilized mini-chaperones

1 reference

https://api.crossref.org/works/10.1016%2FS1387-2656%2804%2910002-1

7 January 2021

The production and characterisation of an immobilised chaperonin system

1 reference

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7 January 2021

Immobilized oxidoreductase as an additive for refolding inclusion bodies: application to antibody fragments.

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7 January 2021

Solubilization and refolding of inclusion body proteins in reverse micelles

1 reference

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7 January 2021

The substrate binding domain of DnaK facilitates slow protein refolding

1 reference

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7 January 2021

Substrate shuttling between the DnaK and GroEL systems indicates a chaperone network promoting protein folding.

1 reference

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7 January 2021

Temperature-controlled activity of DnaK-DnaJ-GrpE chaperones: protein-folding arrest and recovery during and after heat shock depends on the substrate protein and the GrpE concentration

1 reference

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7 January 2021

Role of Hsp70 (DnaK-DnaJ-GrpE) and Hsp100 (ClpA and ClpB) chaperones in refolding and increased thermal stability of bacterial luciferases in Escherichia coli cells

1 reference

https://api.crossref.org/works/10.1016%2FS1387-2656%2804%2910002-1

7 January 2021

Prolyl isomerases catalyze antibody folding in vitro

1 reference

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7 January 2021

DsbA and DsbC-catalyzed oxidative folding of proteins with complex disulfide bridge patterns in vitro and in vivo

1 reference

https://api.crossref.org/works/10.1016%2FS1387-2656%2804%2910002-1

7 January 2021

Effects of DsbA on the disulfide folding of bovine pancreatic trypsin inhibitor and alpha-lactalbumin.

1 reference

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7 January 2021

Assisted refolding of recombinant prochymosin with the aid of protein disulphide isomerase

1 reference

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7 January 2021

A sparse matrix screen to establish initial conditions for protein renaturation

1 reference

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7 January 2021

Overexpression of a glutamate receptor (GluR2) ligand binding domain in Escherichia coli: application of a novel protein folding screen

1 reference

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7 January 2021

A new protein folding screen: application to the ligand binding domains of a glutamate and kainate receptor and to lysozyme and carbonic anhydrase

1 reference

https://api.crossref.org/works/10.1016%2FS1387-2656%2804%2910002-1

7 January 2021

Identification of in vitro folding conditions for procathepsin S and cathepsin S using fractional factorial screens

1 reference

https://api.crossref.org/works/10.1016%2FS1387-2656%2804%2910002-1

7 January 2021

Protein purification by fusion with an environmentally responsive elastin-like polypeptide: effect of polypeptide length on the purification of thioredoxin.

1 reference

https://api.crossref.org/works/10.1016%2FS1387-2656%2804%2910002-1

7 January 2021

Static and dynamic light scattering from aggregating particles

1 reference

https://api.crossref.org/works/10.1016%2FS1387-2656%2804%2910002-1

7 January 2021

Refolding, purification, and characterization of human recombinant PDE4A constructs expressed in Escherichia coli

1 reference

https://api.crossref.org/works/10.1016%2FS1387-2656%2804%2910002-1

7 January 2021

Alternate aggregation pathways of the Alzheimer beta-amyloid peptide: Abeta association kinetics at endosomal pH.

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7 January 2021

Tissue sulfhydryl groups

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7 January 2021

Determination of disulfide structure in agouti-related protein (AGRP) by stepwise reduction and alkylation

1 reference

https://api.crossref.org/works/10.1016%2FS1387-2656%2804%2910002-1

7 January 2021

Expression of atrial natriuretic factor as a cleavable fusion protein with chloramphenicol acetyltransferase in Escherichia coli

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https://api.crossref.org/works/10.1016%2FS1387-2656%2804%2910002-1

7 January 2021

Ubiquitin fusion augments the yield of cloned gene products in Escherichia coli

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https://api.crossref.org/works/10.1016%2FS1387-2656%2804%2910002-1

7 January 2021

A thioredoxin gene fusion expression system that circumvents inclusion body formation in the E. coli cytoplasm.

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7 January 2021

On-column refolding and characterization of soluble human interleukin-15 receptor alpha-chain produced in Escherichia coli

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https://api.crossref.org/works/10.1016%2FS1387-2656%2804%2910002-1

7 January 2021

Molecular cloning, overexpression in Escherichia coli, and purification of 6×his-tagged C-terminal domain of Clostridium difficile toxins A and B

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https://api.crossref.org/works/10.1016%2FS1387-2656%2804%2910002-1

7 January 2021

Purification of recombinant plasminogen activator inhibitor-1 in the active conformation by refolding from inclusion bodies

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7 January 2021

10.1016/S1387-2656(04)10002-1

Identifiers

DOI

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