(Q36084768)

12 August 2017

title

Systematic improvement of amplicon marker gene methods for increased accuracy in microbiome studies (English)

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12 August 2017

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Jonathan B Clayton

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Daryl M Gohl

object named as

Daryl M Gohl

1

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Ryan Hunter

object named as

Ryan Hunter

10

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Timothy J Johnson

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Timothy J Johnson

9

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author name string

Pajau Vangay

2

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12 August 2017

John Garbe

3

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12 August 2017

Allison MacLean

4

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12 August 2017

Adam Hauge

5

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12 August 2017

Aaron Becker

6

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12 August 2017

Trevor J Gould

7

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12 August 2017

Dan Knights

11

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12 August 2017

Kenneth B Beckman

12

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12 August 2017

publication date

25 July 2016

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12 August 2017

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12 August 2017

volume

34

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12 August 2017

issue

9

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12 August 2017

page(s)

942-949

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https://scigraph.springernature.com/pub.10.1038/nbt.3601

12 August 2017

exact match

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cites work

Quantification of the detrimental effect of a single primer-template mismatch by real-time PCR using the 16S rRNA gene as an example.

1 reference

12 December 2020

Bias in template-to-product ratios in multitemplate PCR

1 reference

12 December 2020

Evaluating bias of illumina-based bacterial 16S rRNA gene profiles

1 reference

12 December 2020

Effects of OTU clustering and PCR artifacts on microbial diversity estimates

1 reference

12 December 2020

Frequency of formation of chimeric molecules as a consequence of PCR coamplification of 16S rRNA genes from mixed bacterial genomes.

1 reference

12 December 2020

Improved extraction of PCR-quality community DNA from digesta and fecal samples

1 reference

12 December 2020

Evaluation of methods for the extraction and purification of DNA from the human microbiome

1 reference

12 December 2020

Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies

1 reference

12 December 2020

Reducing the impact of PCR-mediated recombination in molecular evolution and environmental studies using a new-generation high-fidelity DNA polymerase

1 reference

12 December 2020

The microbiome quality control project: baseline study design and future directions

1 reference

12 December 2020

Unusual biology across a group comprising more than 15% of domain Bacteria.

1 reference

12 December 2020

Performance comparison of Illumina and ion torrent next-generation sequencing platforms for 16S rRNA-based bacterial community profiling

1 reference

12 December 2020

The frequency of chimeric molecules as a consequence of PCR co-amplification of 16S rRNA genes from different bacterial species

1 reference

12 December 2020

Evaluation of 16S rDNA-based community profiling for human microbiome research

1 reference

12 December 2020

Reagent and laboratory contamination can critically impact sequence-based microbiome analyses

1 reference

12 December 2020

Storage conditions of intestinal microbiota matter in metagenomic analysis

1 reference

12 December 2020

The long-term stability of the human gut microbiota

1 reference

12 December 2020

Effects of PCR cycle number and DNA polymerase type on the 16S rRNA gene pyrosequencing analysis of bacterial communities.

1 reference

12 December 2020

Analysis, optimization and verification of Illumina-generated 16S rRNA gene amplicon surveys

1 reference

12 December 2020

Comparison of two next-generation sequencing technologies for resolving highly complex microbiota composition using tandem variable 16S rRNA gene regions

1 reference

12 December 2020

Effects of polymerase, template dilution and cycle number on PCR based 16 S rRNA diversity analysis using the deep sequencing method

1 reference

12 December 2020

Coverage evaluation of universal bacterial primers using the metagenomic datasets

1 reference

12 December 2020

Trimmomatic: a flexible trimmer for Illumina sequence data

1 reference

12 December 2020

Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms

1 reference

12 December 2020

A comprehensive benchmarking study of protocols and sequencing platforms for 16S rRNA community profiling

1 reference

12 December 2020

Random sampling process leads to overestimation of β-diversity of microbial communities

1 reference

12 December 2020

The human microbiome: at the interface of health and disease

1 reference

12 December 2020

Discrimination of primer 3'-nucleotide mismatch by taq DNA polymerase during polymerase chain reaction

1 reference

12 December 2020

Chimeric 16S rRNA sequence formation and detection in Sanger and 454-pyrosequenced PCR amplicons

1 reference

12 December 2020

The truth about metagenomics: quantifying and counteracting bias in 16S rRNA studies

1 reference

12 December 2020

Practical innovations for high-throughput amplicon sequencing.

1 reference

12 December 2020

WebLogo: A Sequence Logo Generator

1 reference

12 December 2020

The impact of different DNA extraction kits and laboratories upon the assessment of human gut microbiota composition by 16S rRNA gene sequencing

1 reference

12 December 2020

Optimization of annealing temperature to reduce bias caused by a primer mismatch in multitemplate PCR

1 reference

12 December 2020

PANDAseq: paired-end assembler for illumina sequences

1 reference

12 December 2020

Metagenomics uncovers gaps in amplicon-based detection of microbial diversity

1 reference

12 December 2020

Reducing the effects of PCR amplification and sequencing artifacts on 16S rRNA-based studies

1 reference

12 December 2020

Development of a dual-index sequencing strategy and curation pipeline for analyzing amplicon sequence data on the MiSeq Illumina sequencing platform.

1 reference

12 December 2020

QIIME allows analysis of high-throughput community sequencing data

1 reference

12 December 2020

Generation of multimillion-sequence 16S rRNA gene libraries from complex microbial communities by assembling paired-end illumina reads

1 reference

12 December 2020

Bias caused by template annealing in the amplification of mixtures of 16S rRNA genes by PCR

1 reference

12 December 2020

Optimal enzymes for amplifying sequencing libraries.

1 reference

12 December 2020

Differential amplification of rRNA genes by polymerase chain reaction

1 reference

12 December 2020

Illumina-based analysis of microbial community diversity

1 reference

12 December 2020

Groundtruthing next-gen sequencing for microbial ecology-biases and errors in community structure estimates from PCR amplicon pyrosequencing

1 reference

12 December 2020

Library preparation methodology can influence genomic and functional predictions in human microbiome research

1 reference

12 December 2020

Microbiome profiling by illumina sequencing of combinatorial sequence-tagged PCR products

1 reference

12 December 2020

Insight into biases and sequencing errors for amplicon sequencing with the Illumina MiSeq platform

1 reference

12 December 2020

Analyzing and minimizing PCR amplification bias in Illumina sequencing libraries

1 reference

12 December 2020

Conducting a microbiome study

1 reference

12 December 2020

Polymerase chain reaction primers miss half of rRNA microbial diversity

1 reference

12 December 2020

BIPES, a cost-effective high-throughput method for assessing microbial diversity.

1 reference

12 December 2020

Biopython: freely available Python tools for computational molecular biology and bioinformatics

1 reference

12 December 2020

The Earth Microbiome project: successes and aspirations

1 reference

12 December 2020

Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities

1 reference

12 December 2020

An improved dual-indexing approach for multiplexed 16S rRNA gene sequencing on the Illumina MiSeq platform

1 reference

12 December 2020

Assessment of bias associated with incomplete extraction of microbial DNA from soil.

1 reference

12 December 2020

Effect of sample storage conditions on culture-independent bacterial community measures in cystic fibrosis sputum specimens

1 reference

12 December 2020

Experimental and analytical tools for studying the human microbiome

1 reference

12 December 2020

DNA replication fidelity

1 reference

12 December 2020

A framework for human microbiome research

1 reference

12 December 2020

PCR biases distort bacterial and archaeal community structure in pyrosequencing datasets

1 reference

12 December 2020

Cutadapt removes adapter sequences from high-throughput sequencing reads

1 reference

Crossref

https://api.crossref.org/works/10.1038%2FNBT.3601

7 January 2021

inferred from DOI database lookup

Identifiers

DOI

10.1038/NBT.3601

1 reference

12 August 2017

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