(Q36862643)

16 August 2017

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title

In vivo versus in vitro screening or selection for catalytic activity in enzymes and abzymes (English)

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16 August 2017

Fastrez J

1

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16 August 2017

publication date

1 February 1997

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16 August 2017

published in

Molecular Biotechnology

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16 August 2017

volume

7

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16 August 2017

issue

1

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16 August 2017

page(s)

37-55

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https://scigraph.springernature.com/pub.10.1007/bf02821543

16 August 2017

exact match

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cites work

Changing the substrate specificity of penicillin G acylase from Kluyvera citrophila through selective pressure

1 reference

Crossref

https://api.crossref.org/works/10.1007%2FBF02821543

Construction and characterization of a set of E. coli strains deficient in all known loci affecting the proteolytic stability of secreted recombinant proteins.

21 January 2018

1 reference

Crossref

https://api.crossref.org/works/10.1007%2FBF02821543

A DNA metalloenzyme with DNA ligase activity

21 January 2018

1 reference

12 December 2020

Isolation of a thermostable enzyme variant by cloning and selection in a thermophile

1 reference

12 December 2020

A revised strategy for cloning antibody gene fragments in bacteria

1 reference

12 December 2020

In vitro selection of RNAs that undergo autolytic cleavage with Pb2+

1 reference

12 December 2020

Evolution of antibiotic resistance: several different amino acid substitutions in an active site loop alter the substrate profile of beta-lactamase.

1 reference

12 December 2020

Glutathione Transferases with Novel Active Sites Isolated by Phage Display from a Library of Random Mutants

1 reference

12 December 2020

A rapid and effective procedure for screening protease mutants

1 reference

12 December 2020

Development of an efficient method for generating and screening active trypsin and trypsin variants

1 reference

12 December 2020

A new TEM beta-lactamase double mutant with broadened specificity reveals substrate-dependent functional interactions.

1 reference

12 December 2020

Selection of multiple human immunodeficiency virus type 1 variants that encode viral proteases with decreased sensitivity to an inhibitor of the viral protease

1 reference

12 December 2020

A DNA enzyme with Mg(2+)-dependent RNA phosphoesterase activity

1 reference

12 December 2020

Enzyme evolution in Rhodobacter sphaeroides: selection of a mutant expressing a new galactitol dehydrogenase and biochemical characterization of the enzyme

1 reference

12 December 2020

Alteration of the catalytic efficiency of penicillin amidase from Escherichia coli

1 reference

12 December 2020

Selection of functional signal peptide cleavage sites from a library of random sequences

1 reference

12 December 2020

Selection of amidases with novel substrate specificities from penicillin amidase of Escherichia coli

1 reference

12 December 2020

Catalytic antibodies: perusing combinatorial libraries

1 reference

12 December 2020

Expanding the scope of RNA catalysis.

1 reference

12 December 2020

Direct selection for a catalytic mechanism from combinatorial antibody libraries

1 reference

12 December 2020

Directed evolution of a protein: selection of potent neutrophil elastase inhibitors displayed on M13 fusion phage

1 reference

12 December 2020

Metabolic acquisitions through laboratory selection

1 reference

12 December 2020

Thymidine kinase mutants obtained by random sequence selection

1 reference

12 December 2020

Stability-increasing mutants of glucose dehydrogenase from Bacillus megaterium IWG3.

1 reference

12 December 2020

Applications of combinatorial technologies to drug discovery. 1. Background and peptide combinatorial libraries

1 reference

12 December 2020

Artificial mutants generated by the insertion of random oligonucleotides into the putative nucleoside binding site of the HSV-1 thymidine kinase gene

1 reference

12 December 2020

Directed evolution of an RNA enzyme

1 reference

12 December 2020

Evolutionary optimization of the catalytic properties of a DNA-cleaving ribozyme

1 reference

12 December 2020

Complete mutagenesis of protein coding domains

1 reference

12 December 2020

Protein engineering for unusual environments

1 reference

12 December 2020

Directed evolution of subtilisin E in Bacillus subtilis to enhance total activity in aqueous dimethylformamide

1 reference

12 December 2020

Assembly of combinatorial antibody libraries on phage surfaces: the gene III site

1 reference

12 December 2020

Understanding and increasing protein stability

1 reference

12 December 2020

A second-site mutation at phenylalanine-137 that increases catalytic efficiency in the mutant aspartate-27----serine Escherichia coli dihydrofolate reductase.

1 reference

12 December 2020

Improvement in the alkaline stability of subtilisin using an efficient random mutagenesis and screening procedure

1 reference

12 December 2020

Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase

1 reference

12 December 2020

At the crossroads of chemistry and immunology: catalytic antibodies

1 reference

12 December 2020

Increased antibody expression from Escherichia coli through wobble-base library mutagenesis by enzymatic inverse PCR.

1 reference

12 December 2020

Experimental evolution of penicillin G acylases from Escherichia coli and Proteus rettgeri

1 reference

12 December 2020

Selection and rapid purification of murine antibody fragments that bind a transition-state analog by phage display

1 reference

12 December 2020

Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda

1 reference

12 December 2020

Detection of catalytic monoclonal antibodies

1 reference

12 December 2020

Evolution of a second gene for beta-galactosidase in Escherichia coli

1 reference

12 December 2020

catELISA: a facile general route to catalytic antibodies

1 reference

12 December 2020

Random mutagenesis of staphylococcal nuclease and phage display selection

1 reference

12 December 2020

Functional genetic expression of eukaryotic DNA in Escherichia coli

1 reference

12 December 2020

Isolation of new ribozymes from a large pool of random sequences [see comment]

1 reference

12 December 2020

Mutants generated by the insertion of random oligonucleotides into the active site of the beta-lactamase gene

1 reference

12 December 2020

Phage display of catalytically active staphylococcal nuclease.

1 reference

12 December 2020

Isolation of a Bacillus stearothermophilus mutant exhibiting increased thermostability in its restriction endonuclease

1 reference

12 December 2020

Antibody-selectable filamentous fd phage vectors: affinity purification of target genes

1 reference

12 December 2020

A genetic selection elucidates structural determinants of arginine versus lysine specificity in trypsin

1 reference

12 December 2020

Proteinase trapping: screening for viral proteinase mutants by alpha complementation

1 reference

12 December 2020

An RNA polymerase mutant with reduced accuracy of chain elongation

1 reference

12 December 2020

Encoded reaction cassette for the highly sensitive detection of the making and breaking of chemical bonds

1 reference

12 December 2020

Immuno-PCR: very sensitive antigen detection by means of specific antibody-DNA conjugates

1 reference

12 December 2020

In vitro evolution of new ribozymes with polynucleotide kinase activity

1 reference

12 December 2020

Monoclonal anti-idiotypic antibodies as functional internal images of enzyme active sites: production of a catalytic antibody with a cholinesterase activity

1 reference

12 December 2020

Simple method for selecting catalytic monoclonal antibodies that exhibit turnover and specificity

1 reference

12 December 2020

Forced evolution of glutathione S-transferase to create a more efficient drug detoxication enzyme.

1 reference

12 December 2020

Direct selection of antibodies that coordinate metals from semisynthetic combinatorial libraries

1 reference

12 December 2020

Phage display of enzymes and in vitro selection for catalytic activity.

1 reference

12 December 2020

Phage-enzymes: expression and affinity chromatography of functional alkaline phosphatase on the surface of bacteriophage

1 reference

12 December 2020

Systematic mutation of bacteriophage T4 lysozyme.

1 reference

12 December 2020

Enzyme engineering for nonaqueous solvents: random mutagenesis to enhance activity of subtilisin E in polar organic media

1 reference

12 December 2020

Selection of catalytic antibodies for a biosynthetic reaction from a combinatorial cDNA library by complementation of an auxotrophic Escherichia coli: antibodies for orotate decarboxylation

1 reference

12 December 2020

Random peptide libraries: a source of specific protein binding molecules

1 reference

12 December 2020

Searching for Peptide Ligands with an Epitope Library

1 reference

12 December 2020

An efficient method for generating proteins with altered enzymatic properties: application to beta-lactamase

1 reference

12 December 2020

Catalysis of concerted reactions by antibodies: the Claisen rearrangement

1 reference

12 December 2020

Herpes thymidine kinase mutants with altered catalytic efficiencies obtained by random sequence selection.

1 reference

12 December 2020

Development of an in vivo method to identify mutants of phage T4 lysozyme of enhanced thermostability

1 reference

12 December 2020

Modifying the specificity and activity of the Enterobacter cloacae P99 beta-lactamase by mutagenesis within an M13 phage vector

1 reference

12 December 2020

beta-Galactosidase alpha-complementation. A model of protein-protein interaction

1 reference

12 December 2020

Directed selective pressure on a beta-lactamase to analyse molecular changes involved in development of enzyme function

1 reference

12 December 2020

Site-saturation studies of beta-lactamase: production and characterization of mutant beta-lactamases with all possible amino acid substitutions at residue 71

1 reference

12 December 2020

1 reference

12 December 2020

Selection of beta-lactamase on filamentous bacteriophage by catalytic activity

1 reference

12 December 2020

A genetic screen for mutations that increase the thermal stability of phage T4 lysozyme

1 reference

12 December 2020

Isolation of a high affinity inhibitor of urokinase-type plasminogen activator by phage display of ecotin

1 reference

12 December 2020

Probing beta-lactamase structure and function using random replacement mutagenesis

1 reference

12 December 2020

Substrate phage: selection of protease substrates by monovalent phage display

1 reference

12 December 2020

Posttranscriptional regulatory mechanisms in Escherichia coli

1 reference

12 December 2020

Expression of prostate specific antigen on the surface of a filamentous phage.

1 reference

12 December 2020

Display of peptides and proteins on the surface of bacteriophage lambda

1 reference

12 December 2020

Hormone phage: an enrichment method for variant proteins with altered binding properties

1 reference

12 December 2020

In vivo catalysis of a metabolically essential reaction by an antibody

1 reference

12 December 2020

Abzyme generation using an anti-idiotypic antibody as the "internal image" of an enzyme active site

1 reference

12 December 2020

Permissible amino acid substitutions within the putative nucleoside binding site of herpes simplex virus type 1 encoded thymidine kinase established by random sequence mutagenesis [corrected]

1 reference

12 December 2020

A survey of furin substrate specificity using substrate phage display

1 reference

12 December 2020

Immunogenicity and epitope mapping of foreign sequences via genetically engineered filamentous phage

1 reference

12 December 2020

Screening for thermostable mutant of kanamycin nucleotidyltransferase by the use of a transformation system for a thermophile, Bacillus stearothermophilus

1 reference

12 December 2020

Controlling protein evolution

1 reference

12 December 2020

Trypsin display on the surface of bacteriophage

1 reference

12 December 2020

Direct selection of binding proficient/catalytic deficient variants of BamHI endonuclease

1 reference

12 December 2020

Random mutagenesis of the substrate-binding site of a serine protease can generate enzymes with increased activities and altered primary specificities

1 reference

12 December 2020

Bacterial DD-transpeptidases and penicillin.

1 reference

12 December 2020

Substrate specificity of trypsin investigated by using a genetic selection

1 reference

12 December 2020

Lambda foo: a lambda phage vector for the expression of foreign proteins

1 reference

12 December 2020

Further stabilization of 3-isopropylmalate dehydrogenase of an extreme thermophile, Thermus thermophilus, by a suppressor mutation method

1 reference

12 December 2020

Structural and genetic analysis of protein stability

1 reference

12 December 2020

Linkage of recognition and replication functions by assembling combinatorial antibody Fab libraries along phage surfaces

1 reference

12 December 2020

Selection of guide sequences that direct efficient cleavage of mRNA by human ribonuclease P.

1 reference

12 December 2020

Thermostable mutants of kanamycin nucleotidyltransferase are also more stable to proteinase K, urea, detergents, and water-miscible organic solvents.

1 reference

12 December 2020

In vitro selection and evolution of RNA: applications for catalytic RNA, molecular recognition, and drug discovery.

1 reference

12 December 2020

Phage antibodies: filamentous phage displaying antibody variable domains

1 reference

12 December 2020

From molecular diversity to catalysis: lessons from the immune system

1 reference

12 December 2020

In vitro selection of catalytic RNAs

1 reference

12 December 2020

Artificial evolution and natural ribozymes.

1 reference

12 December 2020

Translational level is a critical factor for the secretion of heterologous proteins in Escherichia coli

1 reference

12 December 2020

Continuous cultures of fused cells secreting antibody of predefined specificity

1 reference

12 December 2020

Combinatorial libraries by cassette mutagenesis

1 reference

12 December 2020

Improved peptide function from random mutagenesis over short 'windows'.

1 reference

12 December 2020

Rapid evolution of a protein in vitro by DNA shuffling

1 reference

12 December 2020

Searching sequence space by definably random mutagenesis: improving the catalytic potency of an enzyme

1 reference

12 December 2020

Hyperthermostable variants of a highly thermostable alpha-amylase

1 reference

12 December 2020

Proteases of enhanced stability: characterization of a thermostable variant of subtilisin

1 reference

12 December 2020

Identification of important residues within the putative nucleoside binding site of HSV-1 thymidine kinase by random sequence selection: analysis of selected mutants in vitro

1 reference

12 December 2020

Peptides on phage: a vast library of peptides for identifying ligands

1 reference

12 December 2020

Overexpression of the phage lambda lysozyme cloned in Escherichia coli: use of a degenerative mixture of synthetic ribosome binding sites and increase of the protein stability in vivo

1 reference

12 December 2020

Substitution of Asp for Asn at position 132 in the active site of TEM beta-lactamase. Activity toward different substrates and effects of neighboring residues

1 reference

12 December 2020

Selection in vitro of an RNA enzyme that specifically cleaves single-stranded DNA

1 reference

12 December 2020

Phage display: protein engineering by directed evolution

1 reference

12 December 2020

Optimization of an anti-HIV hairpin ribozyme by in vitro selection

1 reference

12 December 2020

A genetic approach to the generation of antibodies with enhanced catalytic activities

1 reference

12 December 2020

Applications of combinatorial technologies to drug discovery. 2. Combinatorial organic synthesis, library screening strategies, and future directions

1 reference

12 December 2020

In vitro evolution of a self-alkylatlng ribozyme

1 reference

12 December 2020

A stable phage-display system using a phagemid vector: phage display of hen egg-white lysozyme (HEL), Escherichia coli alkaline, phosphatase, and anti-HEL monoclonal antibody, HyHEL10.

1 reference

12 December 2020

In vitro selection of RNA molecules that bind specific ligands

1 reference

12 December 2020

Identifiers

DOI

10.1007/BF02821543

1 reference

16 August 2017

1 reference