(Q37958819)

25 August 2017

title

Towards dynamic metabolic flux analysis in CHO cell cultures (English)

1 reference

25 August 2017

Woo Suk Ahn

1

1 reference

25 August 2017

Maciek R Antoniewicz

series ordinal

2

1 reference

25 August 2017

publication date

21 November 2011

1 reference

25 August 2017

published in

Biotechnology Journal

1 reference

25 August 2017

volume

7

1 reference

25 August 2017

issue

1

1 reference

25 August 2017

page(s)

61-74

1 reference

Production of recombinant protein therapeutics in cultivated mammalian cells

25 August 2017

cites work

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

A GFP-based method facilitates clonal selection of transfected CHO cells.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

The importance of ammonia in mammalian cell culture

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Effects of ammonia on CHO cell growth, erythropoietin production, and glycosylation

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Effects of ammonium and lactate on growth and metabolism of a recombinant Chinese hamster ovary cell culture

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Effects of ammonia and lactate on hybridoma growth, metabolism, and antibody production

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Effect of culture temperature on erythropoietin production and glycosylation in a perfusion culture of recombinant CHO cells.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Effect of low culture temperature on specific productivity, transcription level, and heterogeneity of erythropoietin in Chinese hamster ovary cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Effects of elevated pCO2 and osmolality on growth of CHO cells and production of antibody-fusion protein B1: a case study

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Impact of dynamic online fed-batch strategies on metabolism, productivity and N-glycosylation quality in CHO cell cultures.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Feed development for fed-batch CHO production process by semisteady state analysis

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Improvement of CHO cell culture medium formulation: simultaneous substitution of glucose and glutamine

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Functional expression of human pyruvate carboxylase for reduced lactic acid formation of Chinese hamster ovary cells (DG44).

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Expression of anti-apoptosis genes alters lactate metabolism of Chinese Hamster Ovary cells in culture

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Metabolic flux analysis and pharmaceutical production

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Elementary metabolite units (EMU): a novel framework for modeling isotopic distributions

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Determination of confidence intervals of metabolic fluxes estimated from stable isotope measurements.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Accurate assessment of amino acid mass isotopomer distributions for metabolic flux analysis.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Measuring deuterium enrichment of glucose hydrogen atoms by gas chromatography/mass spectrometry

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Linking high-resolution metabolic flux phenotypes and transcriptional regulation in yeast modulated by the global regulator Gcn4p

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

13C metabolic flux analysis

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Dynamic metabolic flux analysis (DMFA): a framework for determining fluxes at metabolic non-steady state.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Metabolic flux analysis in a nonstationary system: fed-batch fermentation of a high yielding strain of E. coli producing 1,3-propanediol

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Quantitative characterization of metabolism and metabolic shifts during growth of the new human cell line AGE1.HN using time resolved metabolic flux analysis.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Dynamic metabolic flux analysis demonstrated on cultures where the limiting substrate is changed from carbon to nitrogen and vice versa

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

A procedure for the estimation over time of metabolic fluxes in scenarios where measurements are uncertain and/or insufficient

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Metabolic flux analysis in eukaryotes

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

On the Origin of Cancer Cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Understanding the Warburg effect: the metabolic requirements of cell proliferation

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Metabolism of PER.C6 cells cultivated under fed-batch conditions at low glucose and glutamine levels

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Metabolic shifts by nutrient manipulation in continuous cultures of BHK cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Growth, metabolic, and antibody production kinetics of hybridoma cell culture: 1. Analysis of data from controlled batch reactors

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Effect of culture pH on erythropoietin production by Chinese hamster ovary cells grown in suspension at 32.5 and 37.0 degrees C.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

The glucose transporter of mammalian cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Growth characteristics in fed-batch culture of hybridoma cells with control of glucose and glutamine concentrations.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

High viable cell concentration fed-batch cultures of hybridoma cells through on-line nutrient feeding

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Analysis of cellular metabolism of hybridoma cells at distinct physiological states

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Large scale gene expression profiling of metabolic shift of mammalian cells in culture

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Proteomic investigation of metabolic shift in mammalian cell culture

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Isozymes of mammalian hexokinase: structure, subcellular localization and metabolic function

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Glucose and glutamine metabolism of a murine B-lymphocyte hybridoma grown in batch culture

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Hexokinase 2 is a key mediator of aerobic glycolysis and promotes tumor growth in human glioblastoma multiforme

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

The M2 splice isoform of pyruvate kinase is important for cancer metabolism and tumour growth

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Engineering mammalian cell factories for improved recombinant monoclonal antibody production: lessons from nature?

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Improvement of the primary metabolism of cell cultures by introducing a new cytoplasmic pyruvate carboxylase reaction

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Expression of recombinant cytoplasmic yeast pyruvate carboxylase for the improvement of the production of human erythropoietin by recombinant BHK-21 cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Improving glucose and glutamine metabolism of human HEK 293 and Trichoplusia ni insect cells engineered to express a cytosolic pyruvate carboxylase enzyme

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Impact of temperature reduction and expression of yeast pyruvate carboxylase on hGM-CSF-producing CHO cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Engineering of a mammalian cell line for reduction of lactate formation and high monoclonal antibody production

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Effects of lactate dehydrogenase suppression and glycerol-3-phosphate dehydrogenase overexpression on cellular metabolism

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Down-regulation of lactate dehydrogenase-A by siRNAs for reduced lactic acid formation of Chinese hamster ovary cells producing thrombopoietin

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Metabolic genes in cancer: their roles in tumor progression and clinical implications

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Importance of glucose-6-phosphate dehydrogenase activity for cell growth

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Glucose-6-phosphate dehydrogenase and the oxidative pentose phosphate cycle protect cells against apoptosis induced by low doses of ionizing radiation

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Glucose-6-phosphate dehydrogenase plays a crucial role in protection from redox-stress-induced apoptosis

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

TIGAR, a p53-inducible regulator of glycolysis and apoptosis

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Overexpression of transketolase-like gene 1 is associated with cell proliferation in uterine cervix cancer

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Regulation of glutaminase activity and glutamine metabolism

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

A 1H/15N n.m.r. study of nitrogen metabolism in cultured mammalian cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Elevated glutamate dehydrogenase flux in glucose-deprived hybridoma and myeloma cells: evidence from 1H/15N NMR.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Chemical decomposition of glutamine in cell culture media: effect of media type, pH, and serum concentration

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

The enzymic hydrolysis of glutamine and its spontaneous decomposition in buffer solutions

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

CHO-K1 host cells adapted to growth in glutamine-free medium by FACS-assisted evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Cell death in bioreactors: a role for apoptosis.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Growth inhibition in animal cell culture. The effect of lactate and ammonia

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Influence of ammonium on growth, metabolism, and productivity of a continuous suspension Chinese hamster ovary cell culture

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

The effect of ammonia on the O-linked glycosylation of granulocyte colony-stimulating factor produced by chinese hamster ovary cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Ammonia affects the glycosylation patterns of recombinant mouse placental lactogen-I by chinese hamster ovary cells in a pH-dependent manner

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Chloroquine and ammonium chloride prevent terminal glycosylation of immunoglobulins in plasma cells without affecting secretion

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Effects of ammonia and glucosamine on the heterogeneity of erythropoietin glycoforms.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

High level expression of tissue inhibitor of metalloproteinases in Chinese hamster ovary cells using glutamine synthetase gene amplification

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

High-level expression of a recombinant antibody from myeloma cells using a glutamine synthetase gene as an amplifiable selectable marker

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Characterization of glutamine metabolism in two related murine hybridomas

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Characterisation of G418-induced metabolic load in recombinant CHO and BHK cells: effect on the activity and expression of central metabolic enzymes

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Metabolic effects on recombinant interferon-gamma glycosylation in continuous culture of Chinese hamster ovary cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Analysis of CHO cells metabolic redistribution in a glutamate-based defined medium in continuous culture.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Considerations on the lactate consumption by CHO cells in the presence of galactose

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Metabolic flux analysis of CHO cells at growth and non-growth phases using isotopic tracers and mass spectrometry

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Error propagation from prime variables into specific rates and metabolic fluxes for mammalian cells in perfusion culture.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

A detailed metabolic flux analysis of an underdetermined network of CHO cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Metabolic flux analysis in mammalian cell culture

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Dynamic model of CHO cell metabolism.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Metabolic design of macroscopic bioreaction models: application to Chinese hamster ovary cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Development of a mathematical model for evaluating the dynamics of normal and apoptotic Chinese hamster ovary cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Metabolic flux analysis of CHO cells in perfusion culture by metabolite balancing and 2D [13C, 1H] COSY NMR spectroscopy

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Metabolic flux analysis of CHO cell metabolism in the late non-growth phase.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

An elementary metabolite unit (EMU) based method of isotopically nonstationary flux analysis.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Metabolic flux analysis in Escherichia coli by integrating isotopic dynamic and isotopic stationary 13C labeling data

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Towards a metabolic and isotopic steady state in CHO batch cultures for reliable isotope-based metabolic profiling.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Evaluation of 13C isotopic tracers for metabolic flux analysis in mammalian cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Quantifying reductive carboxylation flux of glutamine to lipid in a brown adipocyte cell line

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Emerging trends in plasma-free manufacturing of recombinant protein therapeutics expressed in mammalian cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Implementation of advanced technologies in commercial monoclonal antibody production

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Metabolic cycling in control of glucose-stimulated insulin secretion

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Systems-level metabolic flux profiling identifies fatty acid synthesis as a target for antiviral therapy

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

13C NMR isotopomer analysis reveals a connection between pyruvate cycling and glucose-stimulated insulin secretion (GSIS)

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

13C metabolic flux analysis in complex systems.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

Effects of ammonia and lactate on growth, metabolism, and productivity of BHK cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100052

21 January 2018

Identifiers

DOI

10.1002/BIOT.201100052

1 reference

25 August 2017

1 reference