(Q39962807)

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Rational vector design and multi-pathway modulation of HEK 293E cells yield recombinant antibody titers exceeding 1 g/l by transient transfection under serum-free conditions

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scholarly article

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Europe PubMed Central

15 September 2017

Rational vector design and multi-pathway modulation of HEK 293E cells yield recombinant antibody titers exceeding 1 g/l by transient transfection under serum-free conditions (English)

1 reference

Europe PubMed Central

15 September 2017

1 reference

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author name string

Gaurav Backliwal

1

1 reference

Europe PubMed Central

15 September 2017

Markus Hildinger

2

1 reference

Europe PubMed Central

15 September 2017

Sebastien Chenuet

3

1 reference

Europe PubMed Central

15 September 2017

Sarah Wulhfard

4

1 reference

Europe PubMed Central

15 September 2017

Maria De Jesus

5

1 reference

Europe PubMed Central

15 September 2017

Florian M Wurm

6

1 reference

Europe PubMed Central

15 September 2017

publication date

10 July 2008

1 reference

Europe PubMed Central

15 September 2017

Nucleic Acids Research

1 reference

Europe PubMed Central

15 September 2017

36

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Europe PubMed Central

15 September 2017

15

1 reference

Europe PubMed Central

15 September 2017

e96

1 reference

Europe PubMed Central

15 September 2017

Coexpression of acidic fibroblast growth factor enhances specific productivity and antibody titers in transiently transfected HEK293 cells

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2528171

22 September 2017

Valproic acid: a viable alternative to sodium butyrate for enhancing protein expression in mammalian cell cultures

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2528171

22 September 2017

Genomic and proteomic exploration of CHO and hybridoma cells under sodium butyrate treatment

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2528171

22 September 2017

High-density transfection with HEK-293 cells allows doubling of transient titers and removes need for a priori DNA complex formation with PEI.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2528171

22 September 2017

High-density transient gene expression in suspension-adapted 293 EBNA1 cells

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2528171

22 September 2017

Recombinant protein production by large-scale transient gene expression in mammalian cells: state of the art and future perspectives

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2528171

22 September 2017

Limiting factors governing protein expression following polyethylenimine-mediated gene transfer in HEK293-EBNA1 cells

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2528171

22 September 2017

New disposable tubes for rapid and precise biomass assessment for suspension cultures of mammalian cells

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2528171

22 September 2017

WPRE-mediated enhancement of gene expression is promoter and cell line specific

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2528171

22 September 2017

Large-scale transient transfection of mammalian cells: a newly emerging attractive option for recombinant protein production

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2528171

22 September 2017

Large-scale transient expression of therapeutic proteins in mammalian cells

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2528171

22 September 2017

Transient gene expression in suspension HEK-293 cells: application to large-scale protein production

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2528171

22 September 2017

Production of recombinant protein therapeutics in cultivated mammalian cells

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2528171

22 September 2017

High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2528171

22 September 2017

Large-scale transient expression in mammalian cells for recombinant protein production

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2528171

22 September 2017

A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2528171

22 September 2017

Balancing GFP reporter plasmid quantity in large-scale transient transfections for recombinant anti-human Rhesus-D IgG1 synthesis

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2528171

25 September 2017

Transient gene expression: recombinant protein production with suspension-adapted HEK293-EBNA cells

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2528171

25 September 2017

Control of culture environment for improved polyethylenimine-mediated transient production of recombinant monoclonal antibodies by CHO cells.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2528171

20 August 2018

Orbital shaker technology for the cultivation of mammalian cells in suspension.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2528171

20 August 2018

Identifiers

10.1093/NAR/GKN423

1 reference

Europe PubMed Central

15 September 2017

1 reference

Europe PubMed Central

15 September 2017

1 reference

Europe PubMed Central

15 September 2017

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