(Q41990162)

English

Differences in the chemical and catalytic characteristics of two crystallographically 'identical' enzyme catalytic sites. Characterization of actinidin and papain by a combination of pH-dependent substrate catalysis kinetics and reactivity probe stu

scientific article published on October 1987

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scholarly article

1 reference

Europe PubMed Central

14 October 2017

Differences in the chemical and catalytic characteristics of two crystallographically 'identical' enzyme catalytic sites. Characterization of actinidin and papain by a combination of pH-dependent substrate catalysis kinetics and reactivity probe stu (English)

1 reference

Europe PubMed Central

14 October 2017

author name string

Salih E

1

1 reference

Europe PubMed Central

14 October 2017

Malthouse JP

2

1 reference

Europe PubMed Central

14 October 2017

Kowlessur D

3

1 reference

Europe PubMed Central

14 October 2017

Jarvis M

4

1 reference

Europe PubMed Central

14 October 2017

O'Driscoll M

5

1 reference

Europe PubMed Central

14 October 2017

Brocklehurst K

6

1 reference

Europe PubMed Central

14 October 2017

language of work or name

0 references

publication date

1 October 1987

1 reference

Europe PubMed Central

14 October 2017

Biochemical Journal

1 reference

Europe PubMed Central

14 October 2017

247

1 reference

Europe PubMed Central

14 October 2017

1

1 reference

Europe PubMed Central

14 October 2017

181-193

1 reference

Europe PubMed Central

14 October 2017

Differences in the interaction of the catalytic groups of the active centres of actinidin and papain. Rapid purification of fully active actinidin by covalent chromatography and characterization of its active centre by use of two-protonic-state reac

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1148386

5 June 2018

The specificity of actinidin and its relationship to the structure of the enzyme

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1148386

5 June 2018

Homology of amino acid sequences of rat liver cathepsins B and H with that of papain

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1148386

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1148386

5 June 2018

Structure of papain refined at 1.65 A resolution

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1148386

5 June 2018

Differences between the electric fields of the catalytic sites of papain and actinidin detected by using the thiol-located nitrobenzofurazan label as a spectroscopic reporter group.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1148386

5 June 2018

A marked gradation in active-centre properties in the cysteine proteinases revealed by neutral and anionic reactivity probes. Reactivity characteristics of the thiol groups of actinidin, ficin, papain and papaya peptidase A towards 4,4'-dipyridyl di

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1148386

5 June 2018

Investigation of the catalytic site of actinidin by using benzofuroxan as a reactivity probe with selectivity for the thiolate-imidazolium ion-pair systems of cysteine proteinases. Evidence that the reaction of the ion-pair of actinidin (pKI 3.0, pK

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1148386

5 June 2018

Covalent chromatography. Preparation of fully active papain from dried papaya latex

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1148386

5 June 2018

Reactions of papain and of low-molecular-weight thiols with some aromatic disulphides. 2,2'-Dipyridyl disulphide as a convenient active-site titrant for papain even in the presence of other thiols

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1148386

5 June 2018

Synthesis of methyl 2- and 4-pyridyl disulfide from 2- and 4-thiopyridone and methyl methanethiosulfonate.

1 reference

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5 June 2018

Preparation of cathepsins B and H by covalent chromatography and characterization of their catalytic sites by reaction with a thiol-specific two-protonic-state reactivity probe. Kinetic study of cathepsins B and H extending into alkaline media and a

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1148386

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1148386

5 June 2018

Thiol proteases. Comparative studies based on the high-resolution structures of papain and actinidin, and on amino acid sequence information for cathepsins B and H, and stem bromelain

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1148386

5 June 2018

X-ray scattering. A new probe of protein dynamics

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1148386

5 June 2018

Chemical evidence for the pH-dependent control of ion-pair geometry in cathepsin B. Benzofuroxan as a reactivity probe sensitive to differences in the mutual disposition of the thiolate and imidazolium components of cysteine proteinase catalytic sit

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1148386

5 June 2018

Substrate-derived two-protonic-state electrophiles as sensitive kinetic specificity probes for cysteine proteinases. Activation of 2-pyridyl disulphides by hydrogen-bonding

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1148386

5 June 2018

What is protein engineering?

1 reference

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5 June 2018

Activation of the transforming potential of p60c-src by a single amino acid change

1 reference

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5 June 2018

A simple high-yield procedure for isolation of human urinary kallikreins

1 reference

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5 June 2018

The equilibrium assumption is valid for the kinetic treatment of most time-dependent protein-modification reactions

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1148386

5 June 2018

A necessary modification to the preparation of papain from any high-quality latex of Carica papaya and evidence for the structural integrity of the enzyme produced by traditional methods

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1148386

5 June 2018

Reactivities of neutral and cationic forms of 2,2'-dipyridyl disulphide towards thiolate anions. Detection of differences between the active centres of actinidin, papain and ficin by a three-protonic-state reactivity probe

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1148386

5 June 2018

Dependence of the catalytic activity of papain on the ionization of two acidic groups

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1148386

5 June 2018

The pH-dependence of second-order rate constants of enzyme modification may provide free-reactant pKa values

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1148386

5 June 2018

Benzofuroxan as a thiol-specific reactivity probe. Kinetics of its reactions with papain, ficin, bromelain and low-molecular-weight thiols

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1148386

5 June 2018

Preparation of fully active ficin from Ficus glabrata by covalent chromatography and characterization of its active centre by using 2,2'-depyridyl disulphide as a reactivity probe

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1148386

5 June 2018

A reporter group delivery system with both absolute and selective specificity for thiol groups and an improved fluorescent probe containing the 7-nitrobenzo-2-oxa-1,3-diazole moiety

1 reference

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5 June 2018

The preparation and properties of two new chromogenic substrates of trypsin

1 reference

https://pubmed.ncbi.nlm.nih.gov/2825655

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Structure of actinidin, after refinement at 1.7 A resolution

1 reference

https://pubmed.ncbi.nlm.nih.gov/2825655

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

New reagents for the introduction of the thiomethyl group at sulfhydryl residues of proteins with concomitant spectrophotometric titration of the sulfhydryls: methyl 3-nitro-2-pyridyl disulfide and methyl 2-pyridyl disulfide

1 reference

https://pubmed.ncbi.nlm.nih.gov/2825655

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

24 Two-protonic-state electrophiles as probes of enzyme mechanism

1 reference

https://pubmed.ncbi.nlm.nih.gov/2825655

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Role of the phenylalanine B25 side chain in directing insulin interaction with its receptor. Steric and conformational effects

1 reference

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based on heuristic

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Molecular dynamics studied by analysis of the X-ray diffuse scattering from lysozyme crystals

1 reference

https://pubmed.ncbi.nlm.nih.gov/2825655

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based on heuristic

inferred from PubMed ID database lookup

Species adaptation in a protein molecule

1 reference

https://pubmed.ncbi.nlm.nih.gov/2825655

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Protein engineering

1 reference

https://pubmed.ncbi.nlm.nih.gov/2825655

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Identifiers

10.1042/BJ2470181

1 reference

Europe PubMed Central

14 October 2017

1 reference

Europe PubMed Central

14 October 2017

1 reference

Europe PubMed Central

14 October 2017

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