(Q67248598)

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:6294455%20AND%20SRC:MED&resulttype=core&format=json

11 September 2019

title

Two-protonic-state electrophiles as probes of enzyme mechanisms (English)

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:6294455%20AND%20SRC:MED&resulttype=core&format=json

11 September 2019

24 Two-protonic-state electrophiles as probes of enzyme mechanism (English)

1 reference

https://api.crossref.org/works/10.1016/S0076-6879(82)87026-2

19 September 2023

main subject

indicators and reagents

1 reference

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19 September 2023

author name string

K. Brocklehurst

series ordinal

1

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A6294455

19 September 2023

language of work or name

English

0 references

publication date

1 January 1982

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:6294455%20AND%20SRC:MED&resulttype=core&format=json

11 September 2019

published in

Methods in Enzymology

1 reference

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11 September 2019

volume

87

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:6294455%20AND%20SRC:MED&resulttype=core&format=json

11 September 2019

page(s)

427-469

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:6294455%20AND%20SRC:MED&resulttype=core&format=json

Intramolecular Inhibition by Enzyme of Site-Specific Modification Reactions can Mask pKa Values Characteristic of the Reaction Pathway: Do the Side Chains of Aspartic Acid-158 and Lysine-156 of Papain Form an Ion-Pair?

11 September 2019

cites work

1 reference

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7 January 2021

Substituted Pyridines as Two-Protonic-State Reactivity Probes, Reporter-Group Delivery Vehicles and Labelling Reagents for the Study of Thiol Enzymes

1 reference

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7 January 2021

Characterization of the papain active centre by using two-protonic-state electrophiles as reactivity probes. Evidence for nucleophilic reactivity in the un-interrupted cysteine-25-histidine-159 interactive system

1 reference

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7 January 2021

A novel reactivity of papain and a convenient active site titration in the presence of other thiols

1 reference

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7 January 2021

Reactions of papain and of low-molecular-weight thiols with some aromatic disulphides. 2,2'-Dipyridyl disulphide as a convenient active-site titrant for papain even in the presence of other thiols

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2882%2987026-2

7 January 2021

Preparation of fully active ficin from Ficus glabrata by covalent chromatography and characterization of its active centre by using 2,2'-depyridyl disulphide as a reactivity probe

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2882%2987026-2

7 January 2021

A convenient method of preparation of high-activity urease from Canavalia ensiformis by covalent chromatography and an investigation of its thiol groups with 2,2'-dipyridyl disulphide as a thiol titrant and reactivity probe

1 reference

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7 January 2021

Reactivities of the various protonic states in the reactions of papain and of L-cysteine with 2,2'- and with 4,4'- dipyridyl disulphide: evidence for nucleophilic reactivity in the un-ionized thiol group of the cysteine-25 residue of papain occasion

1 reference

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7 January 2021

The mutability of stem bromelain: evidence for perturbation by structural transitions of the parameters that characterize the reaction of the essential thiol group of bromelain with 2,2'-dipyridyl disulphide

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2882%2987026-2

7 January 2021

Propapain and its Conversion to Papain: A New Type of Zymogen Activation Mechanism involving Intramolecular Thiol-Disulphide Interchange

1 reference

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7 January 2021

Reactions of L-ergothioneine and some other aminothiones with2,2'-and 4,4'-dipyridyl disulphides and of L-ergothioneine with iodoacetamide. 2-Mercaptoimidazoles, 2- and 4-thiopyridones, thiourea and thioacetamide as highly reactive neutral sulphur n

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2882%2987026-2

7 January 2021

A reporter group delivery system with both absolute and selective specificity for thiol groups and an improved fluorescent probe containing the 7-nitrobenzo-2-oxa-1,3-diazole moiety

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2882%2987026-2

7 January 2021

Covalent chromatography. Preparation of fully active papain from dried papaya latex

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2882%2987026-2

7 January 2021

Immobilization of urease by thiol-disulphide interchange with concomitant purification.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2882%2987026-2

7 January 2021

Specific covalent modification of thiols: Applications in the study of enzymes and other biomolecules

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2882%2987026-2

7 January 2021

Evidence that binding to the s2-subsite of papain may be coupled with catalytically relevant structural change involving the cysteine-25-histidine-159 diad. Kinetics of the reaction of papain with a two-protonic-state reactivity probe containing a h

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2882%2987026-2

7 January 2021

Reactivities of neutral and cationic forms of 2,2'-dipyridyl disulphide towards thiolate anions. Detection of differences between the active centres of actinidin, papain and ficin by a three-protonic-state reactivity probe

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2882%2987026-2

7 January 2021

Evidence for association-activation effects in reactions of papain from studies on its reactivity towards isomeric two-protonic-state reactivity probes

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2882%2987026-2

7 January 2021

A kinetic method for the study of solvent environments of thiol groups in proteins involving the use of a pair of isomeric reactivity probes and a differential solvent effect. Investigation of the active centre of ficin by using 2,2'- and 4,4'- dipy

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2882%2987026-2

7 January 2021

Evidence for a two-state transition in papain that may have no close analogue in ficin. Differences in the disposition of cationic sites and hydrophobic binding areas in the active centres of papain and ficin

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2882%2987026-2

7 January 2021

Whither enzyme mechanisms?

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2882%2987026-2

7 January 2021

PH-dependence of the steady-state rate of a two-step enzymic reaction

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2882%2987026-2

7 January 2021

The Intrinsic PkA-Values of Functional Groups in Enzymes: Improper Deductions from the Ph-Dependence of Steady-State Parameter

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2882%2987026-2

7 January 2021

The Reaction of Glutamate Dehydrogenase with 4-Iodoacetamido Salicylic Acid

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2882%2987026-2

7 January 2021

Benzofuroxan as a thiol-specific reactivity probe. Kinetics of its reactions with papain, ficin, bromelain and low-molecular-weight thiols

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2882%2987026-2

7 January 2021

APPENDIX. Validity of a 'Steady-State' Treatment of Inactivation Kinetics

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2882%2987026-2

7 January 2021

The equilibrium assumption is valid for the kinetic treatment of most time-dependent protein-modification reactions

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2882%2987026-2

7 January 2021

Evolution of enzyme catalytic power. Characteristics of optimal catalysis evaluated for the simplest plausible kinetic model

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2882%2987026-2

7 January 2021

PK of the lysine amino group at the active site of acetoacetate decarboxylase

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2882%2987026-2

7 January 2021

The pH-dependence of second-order rate constants of enzyme modification may provide free-reactant pKa values

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2882%2987026-2

7 January 2021

Estimation of the dissociation constants of enzyme-substrate complexes from steady-state measurements. Interpretation of pH-independence of Km

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2882%2987026-2

7 January 2021

A Note on the Kinetics of Enzyme Action

1 reference

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7 January 2021

The Synthesis of Some New Cysteine-Containing Unsymmetrical Disulfides

1 reference

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7 January 2021

The highly electrophilic character of 4-chloro-7-nitrobenzofurazan and possible consequences for its applications as a protein-labelling reagent

1 reference

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7 January 2021

Determination of sulfhydryl groups with 2,2′- or 4,4′-dithiodipyridine

1 reference

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7 January 2021

The Enzymatic Synthesis of L-Cysteine in Higher Plant Tissues

1 reference

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7 January 2021

Simple alkanethiol groups for temporary blocking of sulfhydryl groups of enzymes

1 reference

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7 January 2021

Protein thiolation and reversible protein-protein conjugation. N-Succinimidyl 3-(2-pyridyldithio)propionate, a new heterobifunctional reagent

1 reference

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7 January 2021

Reversible, Covalent Immobilization of Enzymes by Thiol‐Disulphide Interchange

1 reference

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7 January 2021

A thiol-labelling reagent and reactivity probe containing electrophilic mercury and a chromophoric leaving group

1 reference

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7 January 2021

Interactions between cysteine residues as probes of protein conformation: The bisulphide bond between Cys-14 and Cys-38 of the pancreatic trypsin inhibitor

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2882%2987026-2

7 January 2021

Rates of thiol-disulfide interchange reactions involving proteins and kinetic measurements of thiol pKa values.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2882%2987026-2

7 January 2021

Evidence that the active centre of chymopapain A is different from the active centres of some other cysteine proteinases and that the Brønsted coefficient (beta nuc.) for the reactions of thiolate anions with 2,2'-dipyridyl disulphide may be decreas

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2882%2987026-2

7 January 2021

Determination of the reactive sulfhydryl groups in heme proteins with 4,4′-dipyridinedisulfide

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2882%2987026-2

7 January 2021

A spectrophotometric method for the detection of contaminant chymopapains in preparations of papain. Selective modification of one type of thiol group in the chymopapains by a two-protonic-state reagent

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2882%2987026-2

7 January 2021

A convenient spectrophotometric assay for the determination of L-ergothioneine in blood

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2882%2987026-2

7 January 2021

Evidence that the lack of high catalytic activity of thiolsubtilisin towards specific substrates may be due to an inappropriately located proton-distribution system. Demonstration of highly nucleophilic character of the thiol group of thiolsubtilisi

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2882%2987026-2

7 January 2021

Mechanism of action of papain with a specific anilide substrate

1 reference

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7 January 2021

4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole as a reactivity probe for the investigation of the thiol proteinases. evidence that ficin and bromelain may lack carboxyl groups conformationally equivalent to that of aspartic acid-158 of papain

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2882%2987026-2

7 January 2021

Purification of procollagen type II by covalent chromatography with activated thiol-sepharose 4B.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2882%2987026-2

7 January 2021

The separation of two soft‐tissue collagens by covalent chromatography

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2882%2987026-2

7 January 2021

Thiol-disulfide interchange chromatography using sepharose-linked thiol compounds to separate plasma proteins

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2882%2987026-2

7 January 2021

Use of thiol-disulphide interchange reactions in preparative work on plasma proteins

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2882%2987026-2

7 January 2021

Synthesis of metallothionein in a polysomal cell-free system

1 reference

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7 January 2021

Cell-free synthesis of metallothionein directed by rat liver polyadenylated messenger ribonucleic acid

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2882%2987026-2

7 January 2021

Specific isolation of cysteine peptides by covalent chromatography on thiol agarose derivatives

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2882%2987026-2

7 January 2021

Resolution of Ox Liver Thiol-Disulphide Oxidoreductases by a New Application of Covalent Chromatography

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2882%2987026-2

7 January 2021

Resolution of thiol-containing proteins by sequential-elution covalent chromatography

1 reference

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7 January 2021

Biochimica et Biophysica Acta

39

92

111

Hollaway

1964

1 reference

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Journal of Biological Chemistry

19 September 2023

102

253

1799

Taniguchi

1978

1 reference

https://api.crossref.org/works/10.1016/S0076-6879(82)87026-2

19 September 2023

Identifiers

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:6294455%20AND%20SRC:MED&resulttype=core&format=json

11 September 2019

1 reference